FastQCFastQC Report
Thu 2 Feb 2017
SRR4062023_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062023_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181889
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA123221.0425682953306106No Hit
GTCCTACAGTGGACATTTCTAAATT115140.9742031612105705No Hit
GTCCTAAAGTGTGTATTTCTCATTT108780.9203910011853906No Hit
CTTTAGGACGTGAAATATGGCGAGG96940.8202123888114704No Hit
GTCCTACAGTGTGCATTTCTCATTT75400.6379617713677004No Hit
CTGTAGGACCTGGAATATGGCGAGA53920.45621881581096024No Hit
GATATACACTGTTCTACAAATCCCG45250.3828616731351252No Hit
CTGAAGGACCTGGAATATGGCGAGA42950.3634013007989752No Hit
TTGTAGAACAGTGTATATCAATGAG40820.3453793038094102No Hit
GTCCTTCAGTGTGCATTTCTCATTT39290.3324339256901452No Hit
ATTTAGAAATGTCCACTGTAGGACG37330.3158503040471652No Hit
GTGTATATCAATGAGTTACAATGAA34180.28919805497809015No Hit
GAATATGGCAAGAAAACTGAAAATC33570.28403682579328515No Hit
GTGTATATCAATGAGTTACAATGAG33000.2792140378665002No Hit
TTTCTAAATTTTCCACCTTTTTCAG29410.24883893495920512No Hit
ATTCCAGGTCCTTCAGTGTGCATTT26230.22193285494661513No Hit
GTTCTACAGTGTGGTTTTTATCATT25830.21854844236641513No Hit
CTGTAGGACATGGAATATGGCAAGA25480.21558708135874013No Hit
GGAATATGGCGAGAAAACTGAAAAT24930.2109335140609651No Hit
GAAATATGGCGAGGAAAACTGAAAA22890.1936730099019451No Hit
GTTCTACAAATCCCGTTTCCAACGA22510.19045781795075511No Hit
TTCCAGGTCCTTCAGTGTGCATTTC22400.1895271044912001No Hit
GATATACACTGTTCTACAATGCCGG21500.18191217618575012No Hit
ACCTGGAATATGGCGAGAAAACTGA21420.18123529366971008No Hit
GACCTGGAATATGGCGAGAAAACTG21070.1782739326620351No Hit
GCCATATTCCACGTCCTACAGTGGA20610.1743818581948051No Hit
CCATATTTCACGTCCTAAAGTGTGT19330.16355173793816508No Hit
CCATATTCCAGGTCCTTCAGTGTGC18600.1573751849793001No Hit
GTCCACTGTAGGACGTGGAATATGG17920.15162168359296008No Hit
ATACACACTTTAGGACGTGAAATAT17380.14705272660969007No Hit
ACAGTGGACATTTCTAAATTTTCCA16960.14349909340048006No Hit
GCGCAAGACGGACCAGAGCGAAAGC16490.1395224086187451No Hit
GTAGGACGTGGAATATGGCAAGAAA16270.1376609816996351No Hit
CCACTGTAGGACGTGGAATATGGCA16250.13749176107062508No Hit
CCTAAAGTGTGTATTTCTCATTTTC15970.13512267226448507No Hit
GTCCTACAGTGGACATTTCTAAATA15810.1337689072324051No Hit
CTGTAGAACATATTAGATGAGTGAG15560.13165364936978005No Hit
CACTTTAGGACGTGAAATATGGCGA14890.12598475829794506No Hit
GTGTATTTCTCATTTTCCGTGATTT14730.12463099326586508No Hit
GGGTAGGCACACGCTGAGCCAGTCA14610.12361566949180505No Hit
TCCTAAAGTGTGTATTTCTCATTTT14590.12344644886279506No Hit
GTATCAACGCAGAGTACTTTTTTTT14450.12226190445972507No Hit
ATTCCACGTCCTACAGTGGACATTT14150.11972359502457507No Hit
CTACAGTGGACATTTCTAAATTTTC14140.11963898471007008No Hit
GAAATACACACTTTAGGACGTGAAA13930.11786216810546507No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13550.11464697615427508No Hit
TCGTAGTTCCGACCATAAACGATGC13520.11439314521076006No Hit
GATTAAGAGGGACGGCCGGGGGCAT13380.11320860080769007No Hit
GAATAGGACCGCGGTTCTATTTTGT13350.11295476986417506No Hit
ATCAGATACCGTCGTAGTTCCGACC12990.10990879854199506No Hit
ATGTTCTACAGTGTGGTTTTTATCA12860.10880886445343006No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC12730.10770893036486505No Hit
GTATCTGATCGTCTTCGAACCTCCG12490.10567828281674507No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12420.10508601061521006No Hit
TTGATATACACTGTTCTACAAATCC12080.10220925992204005No Hit
GAACTACGACGGTATCTGATCGTCT12060.10204003929303006No Hit
ATATTTCACGTCCTAAAGTGTGTAT12030.10178620834951504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGACA307.711272E-419.0036459
CGCAAGG402.761725E-416.62396216
GGACTGT350.00216639316.2895286
AGCGGAC350.002167619816.2881478
TCGCGAG350.002173147916.28193918
GGTATCA6200.015.3177321
GTGTAGG1750.015.19518851
GCAAGAC3200.014.8428243
GGTTCCG1350.014.7799858
TTGTAGA6750.014.6324041
AAGACGG3200.014.5484335
CAAGACG3200.014.54724
TCTATAC400.00528029314.2491113
GCGTTAT1203.6379788E-1214.2454891
ATCATAT602.5749037E-514.2454891
GGGTTCC1500.013.9365967
CCAACGT1500.013.93010219
TAGGACC23000.013.878574
CGGAATA551.9570407E-413.82024711
GACCTAC551.9647925E-413.8138081
Produced by FastQC (version 0.11.5)