##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062023_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181889
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.469574553955574	32.0	32.0	32.0	32.0	32.0
2	31.194546188347637	32.0	32.0	32.0	32.0	32.0
3	31.192627226414665	32.0	32.0	32.0	32.0	32.0
4	31.197271486577844	32.0	32.0	32.0	32.0	32.0
5	31.214092017101436	32.0	32.0	32.0	32.0	32.0
6	34.902642295511676	36.0	36.0	36.0	36.0	36.0
7	34.83853306021124	36.0	36.0	36.0	36.0	36.0
8	34.83616820192082	36.0	36.0	36.0	36.0	36.0
9	34.85761014782268	36.0	36.0	36.0	36.0	36.0
10	34.66140982782647	36.0	36.0	36.0	32.0	36.0
11	34.794605923229675	36.0	36.0	36.0	36.0	36.0
12	34.68706452128753	36.0	36.0	36.0	32.0	36.0
13	34.72400623070356	36.0	36.0	36.0	32.0	36.0
14	34.73028516214298	36.0	36.0	36.0	32.0	36.0
15	34.669930086497125	36.0	36.0	36.0	32.0	36.0
16	34.688517280387586	36.0	36.0	36.0	32.0	36.0
17	34.613556772251876	36.0	36.0	36.0	32.0	36.0
18	34.635328698380306	36.0	36.0	36.0	32.0	36.0
19	34.51230868550262	36.0	36.0	36.0	32.0	36.0
20	34.46454277855196	36.0	36.0	36.0	32.0	36.0
21	34.42098708085108	36.0	36.0	36.0	32.0	36.0
22	34.329002977436964	36.0	36.0	36.0	32.0	36.0
23	34.35872319650999	36.0	36.0	36.0	32.0	36.0
24	34.27133850979237	36.0	36.0	36.0	32.0	36.0
25	33.93077860949717	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	14.0
5	103.0
6	270.0
7	44.0
8	222.0
9	155.0
10	89.0
11	20.0
12	54.0
13	50.0
14	128.0
15	176.0
16	333.0
17	463.0
18	635.0
19	843.0
20	1141.0
21	1609.0
22	2290.0
23	3181.0
24	4597.0
25	6352.0
26	8863.0
27	11617.0
28	15833.0
29	21755.0
30	29613.0
31	41869.0
32	62076.0
33	94735.0
34	238132.0
35	634627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.1901903663573	19.431515408937802	12.617366075545805	25.760928149159096
2	11.809619218129699	19.571463866166223	48.53488159582497	20.08403531987911
3	20.705941281835848	24.1216770187912	28.958196369691414	26.214185329681534
4	10.88400029447778	16.73158434969067	38.97168020728528	33.41273514854627
5	11.37511881616357	40.20984436881214	35.82581359976368	12.589223215260608
6	31.679349887561298	38.51398033000081	17.802653146928936	12.004016635508954
7	28.256199845162687	30.188447510321076	21.02001196008111	20.535340684435123
8	22.491961469263842	44.63730894724895	18.83752172690341	14.033207856583802
9	26.392073973983727	14.15697021810126	20.36410923276983	39.08684657514519
10	17.36591532421713	27.966952568242302	32.80942992248207	21.8577021850585
11	35.91325288137431	18.887681902376446	28.391344946388898	16.80772026986034
12	27.62290880283331	22.050167549957848	33.10905786510428	17.217865782104564
13	32.140487920597465	22.09816870920051	25.265967135891692	20.495376234310335
14	18.928598959908445	26.781891665459522	24.95660637727419	29.33290299735784
15	20.477828134041992	40.6369121301547	20.479946316776772	18.405313419026534
16	18.867278768296547	26.38608149344213	35.70603195150176	19.040607786759562
17	18.579265064068622	31.763888594726254	30.51640368526951	19.140442655935612
18	19.259300045319588	25.762533830860523	38.39636426783453	16.58180185598536
19	26.125106717439323	23.67628027048636	25.392906508747444	24.805706503326874
20	25.44524779652203	28.315380009434705	28.01794118469071	18.221431009352553
21	22.416050543289547	26.09238039584339	24.611905790289388	26.879663270577677
22	23.905805184916023	32.94235132985549	25.649554427896597	17.502289057331886
23	19.317092502955393	31.926204936607245	29.239507084475125	19.517195475962236
24	25.33868017209255	26.44711880483756	30.501287306480574	17.712913716589316
25	21.462580137026908	29.667680112467075	30.38702902294227	18.48271072756375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	311.0
2	350.0
3	389.0
4	389.0
5	389.0
6	678.5
7	968.0
8	968.0
9	968.0
10	1210.0
11	1452.0
12	1452.0
13	1452.0
14	1960.0
15	2468.0
16	2468.0
17	2468.0
18	5067.0
19	7666.0
20	7666.0
21	7666.0
22	17826.0
23	27986.0
24	27986.0
25	27986.0
26	51876.5
27	75767.0
28	75767.0
29	75767.0
30	109849.0
31	143931.0
32	143931.0
33	143931.0
34	148236.5
35	152542.0
36	152542.0
37	152542.0
38	149496.5
39	146451.0
40	146451.0
41	146451.0
42	146230.5
43	146010.0
44	146010.0
45	146010.0
46	146404.5
47	146799.0
48	146799.0
49	146799.0
50	127585.5
51	108372.0
52	108372.0
53	108372.0
54	83863.0
55	59354.0
56	59354.0
57	59354.0
58	54159.5
59	48965.0
60	48965.0
61	48965.0
62	45462.0
63	41959.0
64	41959.0
65	41959.0
66	35697.5
67	29436.0
68	29436.0
69	29436.0
70	22997.5
71	16559.0
72	16559.0
73	16559.0
74	13632.5
75	10706.0
76	10706.0
77	10706.0
78	9344.5
79	7983.0
80	7983.0
81	7983.0
82	5509.0
83	3035.0
84	3035.0
85	3035.0
86	2356.5
87	1678.0
88	1678.0
89	1678.0
90	1116.5
91	555.0
92	555.0
93	555.0
94	341.5
95	128.0
96	128.0
97	128.0
98	273.5
99	419.0
100	419.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.461031450500005E-4
2	4.2305157252500025E-4
3	5.076618870300003E-4
4	0.011507002772680006
5	0.03739775901121002
6	0.06777286191850504
7	0.10957035728397506
8	0.11210866671912507
9	0.12471560358037007
10	0.13926857767523007
11	0.14578357189211508
12	0.1390993570462201
13	0.13317663503087007
14	0.11980820533908007
15	0.1380840332721601
16	0.12480021389487507
17	0.12810001616057007
18	0.11710067527492007
19	0.10161698772050505
20	0.09662497916471005
21	0.09467894193109505
22	0.10762432005036006
23	0.08435648356148504
24	0.09552504507614505
25	0.09298673564099505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1181889.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.63407079868213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.57551704413233	24.663367738652223
2	13.5062148169842	8.81525541119344
3	4.222892176459749	4.134304867853649
4	1.8328280250440379	2.392505581243835
5	0.9588355746041558	1.564535401296354
6	0.6481009355078973	1.2690103088434104
7	0.42852795623782053	0.9789228164155714
8	0.3211605387935552	0.8384620608585424
9	0.2618266929784971	0.7690023752080576
>10	1.7213008117007238	11.172776797470094
>50	0.22932412486727083	5.213892772282818
>100	0.2438668612631907	16.225918173202682
>500	0.030905384972610285	6.87521667599519
>1k	0.017140801749514947	10.22705431285734
>5k	7.791273522506794E-4	1.917636248903688
>10k+	7.791273522506794E-4	2.942138457723089
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGA	12322	1.0425682953306106	No Hit
GTCCTACAGTGGACATTTCTAAATT	11514	0.9742031612105705	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	10878	0.9203910011853906	No Hit
CTTTAGGACGTGAAATATGGCGAGG	9694	0.8202123888114704	No Hit
GTCCTACAGTGTGCATTTCTCATTT	7540	0.6379617713677004	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5392	0.45621881581096024	No Hit
GATATACACTGTTCTACAAATCCCG	4525	0.3828616731351252	No Hit
CTGAAGGACCTGGAATATGGCGAGA	4295	0.3634013007989752	No Hit
TTGTAGAACAGTGTATATCAATGAG	4082	0.3453793038094102	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3929	0.3324339256901452	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3733	0.3158503040471652	No Hit
GTGTATATCAATGAGTTACAATGAA	3418	0.28919805497809015	No Hit
GAATATGGCAAGAAAACTGAAAATC	3357	0.28403682579328515	No Hit
GTGTATATCAATGAGTTACAATGAG	3300	0.2792140378665002	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2941	0.24883893495920512	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2623	0.22193285494661513	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2583	0.21854844236641513	No Hit
CTGTAGGACATGGAATATGGCAAGA	2548	0.21558708135874013	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2493	0.2109335140609651	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2289	0.1936730099019451	No Hit
GTTCTACAAATCCCGTTTCCAACGA	2251	0.19045781795075511	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	2240	0.1895271044912001	No Hit
GATATACACTGTTCTACAATGCCGG	2150	0.18191217618575012	No Hit
ACCTGGAATATGGCGAGAAAACTGA	2142	0.18123529366971008	No Hit
GACCTGGAATATGGCGAGAAAACTG	2107	0.1782739326620351	No Hit
GCCATATTCCACGTCCTACAGTGGA	2061	0.1743818581948051	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1933	0.16355173793816508	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1860	0.1573751849793001	No Hit
GTCCACTGTAGGACGTGGAATATGG	1792	0.15162168359296008	No Hit
ATACACACTTTAGGACGTGAAATAT	1738	0.14705272660969007	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1696	0.14349909340048006	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1649	0.1395224086187451	No Hit
GTAGGACGTGGAATATGGCAAGAAA	1627	0.1376609816996351	No Hit
CCACTGTAGGACGTGGAATATGGCA	1625	0.13749176107062508	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1597	0.13512267226448507	No Hit
GTCCTACAGTGGACATTTCTAAATA	1581	0.1337689072324051	No Hit
CTGTAGAACATATTAGATGAGTGAG	1556	0.13165364936978005	No Hit
CACTTTAGGACGTGAAATATGGCGA	1489	0.12598475829794506	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1473	0.12463099326586508	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1461	0.12361566949180505	No Hit
TCCTAAAGTGTGTATTTCTCATTTT	1459	0.12344644886279506	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1445	0.12226190445972507	No Hit
ATTCCACGTCCTACAGTGGACATTT	1415	0.11972359502457507	No Hit
CTACAGTGGACATTTCTAAATTTTC	1414	0.11963898471007008	No Hit
GAAATACACACTTTAGGACGTGAAA	1393	0.11786216810546507	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1355	0.11464697615427508	No Hit
TCGTAGTTCCGACCATAAACGATGC	1352	0.11439314521076006	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1338	0.11320860080769007	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1335	0.11295476986417506	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1299	0.10990879854199506	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1286	0.10880886445343006	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1273	0.10770893036486505	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1249	0.10567828281674507	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1242	0.10508601061521006	No Hit
TTGATATACACTGTTCTACAAATCC	1208	0.10220925992204005	No Hit
GAACTACGACGGTATCTGATCGTCT	1206	0.10204003929303006	No Hit
ATATTTCACGTCCTAAAGTGTGTAT	1203	0.10178620834951504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGACA	30	7.711272E-4	19.003645	9
CGCAAGG	40	2.761725E-4	16.623962	16
GGACTGT	35	0.002166393	16.289528	6
AGCGGAC	35	0.0021676198	16.288147	8
TCGCGAG	35	0.0021731479	16.281939	18
GGTATCA	620	0.0	15.317732	1
GTGTAGG	175	0.0	15.1951885	1
GCAAGAC	320	0.0	14.842824	3
GGTTCCG	135	0.0	14.779985	8
TTGTAGA	675	0.0	14.632404	1
AAGACGG	320	0.0	14.548433	5
CAAGACG	320	0.0	14.5472	4
TCTATAC	40	0.005280293	14.249111	3
GCGTTAT	120	3.6379788E-12	14.245489	1
ATCATAT	60	2.5749037E-5	14.245489	1
GGGTTCC	150	0.0	13.936596	7
CCAACGT	150	0.0	13.930102	19
TAGGACC	2300	0.0	13.87857	4
CGGAATA	55	1.9570407E-4	13.820247	11
GACCTAC	55	1.9647925E-4	13.813808	1
>>END_MODULE