FastQCFastQC Report
Thu 2 Feb 2017
SRR4062023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181889
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA60390.5109616892956953No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC43520.3682240887257602No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA39900.3375951548749502No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA36560.3093353098302802No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG27210.2302246657681051No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA23460.19849579782873014No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC22170.1875810672575851No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA18150.15356772082657508No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA18090.15306005893954508No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC17890.15136785264944508No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA17550.14849110195627507No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT16760.1418068871103801No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA15200.1286076780476001No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC14720.12454638295136006No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA14690.12429255200784507No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT14590.12344644886279506No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA14560.12319261791928007No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14270.12073891879863506No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14080.11913132282304005No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13530.11447775552526505No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT13300.11253171829165005No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG13100.11083951200155007No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA12170.10297075275258506No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11960.10119393614798006No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11960.10119393614798006No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA207.8579487E-443.9984327
CGGATCC300.005745960429.3322930
GGTATCA8100.028.8000661
GTCGAGA804.318281E-927.49902219
TTGCGCT407.033502E-427.49902212
TCATCGC757.000199E-826.40464644
TAGTCCT508.343295E-526.399064
TAGGACC24550.026.2556864
GTATCAA19400.025.2973921
ATTGGCG450.001398485324.44357316
GATTGCG450.001398485324.44357310
TTACGCT450.001398485324.44357316
TGTAGGA65600.024.2135772
GACGTGT551.5939737E-423.9991467
CTGTAGG63900.023.8673951
CTAGTAG651.8138458E-523.6914653
GTGTAGG2350.023.4123381
AGGACCT37050.023.2758245
GTAGGAC67850.023.0205523
TAGGACA13700.022.8021094
Produced by FastQC (version 0.11.5)