FastQCFastQC Report
Thu 2 Feb 2017
SRR4062022_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062022_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679859
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11570.1701823466336402No Hit
TATCAACGCAGAGTACTTTTTTTTT8290.12193704871157109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGT250.006024877519.0020646
GTCTAGC402.7652667E-416.6194611
GCACCGT500.001496805913.3014446
AACAGTT751.4740352E-512.6680417
TATACTG701.0880535E-412.21561055
GTATCAA4850.012.1402831
TGCACCG550.003062896412.0922215
CTTACAC550.003067969812.089553
TAAGACT1052.7123315E-711.7614494
GTCCTAG1052.722736E-711.7579851
TTAGGGT658.01582E-411.6918554
GCATAAG658.0356543E-411.6884121
GTCTTAT658.0356543E-411.6884121
GTTATAG658.0356543E-411.6884121
GGTATCA1950.011.6884111
TTAGACT752.071283E-411.3995584
GTATTAG752.076958E-411.3962011
GCCCTAG752.076958E-411.3962011
CTAGTAC700.001490927110.8559233
GTCACAC803.7686952E-410.6862983
Produced by FastQC (version 0.11.5)