##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062022_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 679859 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27443631694219 32.0 32.0 32.0 32.0 32.0 2 30.84473103981855 32.0 32.0 32.0 32.0 32.0 3 30.88012808538241 32.0 32.0 32.0 32.0 32.0 4 30.88791793592495 32.0 32.0 32.0 32.0 32.0 5 30.812843545499877 32.0 32.0 32.0 32.0 32.0 6 34.47025927434954 36.0 36.0 36.0 32.0 36.0 7 34.413547514999436 36.0 36.0 36.0 32.0 36.0 8 34.350733019640835 36.0 36.0 36.0 32.0 36.0 9 34.489301458096456 36.0 36.0 36.0 32.0 36.0 10 34.19451974600616 36.0 36.0 36.0 32.0 36.0 11 34.46482138208069 36.0 36.0 36.0 32.0 36.0 12 34.31716135257458 36.0 36.0 36.0 32.0 36.0 13 34.38174386159483 36.0 36.0 36.0 32.0 36.0 14 34.29908848746578 36.0 36.0 36.0 32.0 36.0 15 34.25005920345248 36.0 36.0 36.0 32.0 36.0 16 34.2543174393514 36.0 36.0 36.0 32.0 36.0 17 34.213591347617665 36.0 36.0 36.0 32.0 36.0 18 34.196789334259016 36.0 36.0 36.0 32.0 36.0 19 34.20290677919981 36.0 36.0 36.0 32.0 36.0 20 34.175476091366 36.0 36.0 36.0 32.0 36.0 21 34.16656100750303 36.0 36.0 36.0 32.0 36.0 22 34.14932360974849 36.0 36.0 36.0 32.0 36.0 23 34.11487823210401 36.0 36.0 36.0 32.0 36.0 24 34.07794998668842 36.0 36.0 36.0 32.0 36.0 25 33.732390098535134 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 52.0 6 142.0 7 25.0 8 132.0 9 90.0 10 52.0 11 13.0 12 28.0 13 21.0 14 103.0 15 158.0 16 260.0 17 320.0 18 477.0 19 663.0 20 939.0 21 1280.0 22 1824.0 23 2662.0 24 3850.0 25 5284.0 26 7119.0 27 9556.0 28 12698.0 29 16865.0 30 22310.0 31 30645.0 32 43133.0 33 62479.0 34 138557.0 35 318114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16885367191833 17.115859551165038 11.856015085702856 25.859271691213788 2 16.772644835605135 18.300024858168705 38.38719024736084 26.54014005886532 3 20.80634841252914 20.836060630575638 27.284641577983543 31.07294937891168 4 13.625237205607615 14.672766589682107 34.01344532870445 37.68855087600583 5 15.412503328953584 35.71477967109203 33.33617796401935 15.536539035935043 6 36.53702654372319 33.28893435083046 15.724598753394314 14.449440352052042 7 31.820370373097077 29.456249180965116 18.633209306297697 20.090171139640113 8 27.827317409319406 34.403997985693714 17.970615865312407 19.79806873967447 9 26.18299735369092 16.022171858409948 18.458631724194806 39.33619906370433 10 16.885479138928492 27.02424856546995 30.808373885072637 25.281898410528918 11 36.82567831698145 21.687290016688195 21.219637573038476 20.267394093291877 12 24.537544330941525 23.414690184157504 28.337044760995838 23.71072072390513 13 30.063960860607146 19.626985798420936 24.300708827128947 26.008344513842967 14 22.675993078820454 19.456024739535398 25.341530758752718 32.526451422891434 15 24.983320298864896 27.21685378861185 21.903503694572212 25.896322217951038 16 25.3004897961588 26.307620262186738 23.696650359763083 24.695239581891386 17 24.080938390510063 26.35180808058495 25.045248035815533 24.52200549308946 18 24.281744810494896 25.83053433091203 25.651329458659927 24.23639139993315 19 25.232553688320635 25.29247730411456 25.435734498629266 24.03923450893554 20 25.14862330213152 25.191318455789908 25.38167995123919 24.278378290839385 21 25.18023847276143 24.691161726579306 25.320836995519986 24.80776280513928 22 24.949424454711696 25.106525202375803 25.226669729174212 24.71738061373829 23 24.589900881365313 25.04640895527222 25.380877302230132 24.982812861132334 24 24.36612045993257 25.153188168956024 25.362395653902215 25.118295717209193 25 24.700632741812488 25.160540936834757 25.308789193040727 24.83003712831203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 268.0 1 268.0 2 234.0 3 200.0 4 200.0 5 200.0 6 382.0 7 564.0 8 564.0 9 564.0 10 790.0 11 1016.0 12 1016.0 13 1016.0 14 1385.5 15 1755.0 16 1755.0 17 1755.0 18 2801.5 19 3848.0 20 3848.0 21 3848.0 22 6074.5 23 8301.0 24 8301.0 25 8301.0 26 12295.0 27 16289.0 28 16289.0 29 16289.0 30 21497.0 31 26705.0 32 26705.0 33 26705.0 34 33253.5 35 39802.0 36 39802.0 37 39802.0 38 47978.0 39 56154.0 40 56154.0 41 56154.0 42 63838.5 43 71523.0 44 71523.0 45 71523.0 46 78477.5 47 85432.0 48 85432.0 49 85432.0 50 89396.0 51 93360.0 52 93360.0 53 93360.0 54 90672.5 55 87985.0 56 87985.0 57 87985.0 58 80313.0 59 72641.0 60 72641.0 61 72641.0 62 62232.5 63 51824.0 64 51824.0 65 51824.0 66 42034.5 67 32245.0 68 32245.0 69 32245.0 70 24484.5 71 16724.0 72 16724.0 73 16724.0 74 12078.5 75 7433.0 76 7433.0 77 7433.0 78 5395.5 79 3358.0 80 3358.0 81 3358.0 82 2393.5 83 1429.0 84 1429.0 85 1429.0 86 989.0 87 549.0 88 549.0 89 549.0 90 360.0 91 171.0 92 171.0 93 171.0 94 109.0 95 47.0 96 47.0 97 47.0 98 141.5 99 236.0 100 236.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001470893229331376 2 2.941786458662752E-4 3 5.883572917325504E-4 4 0.010149163282386495 5 0.03177129375355772 6 0.06089497969431897 7 0.10384506199079516 8 0.10546304454305967 9 0.11767145834651008 10 0.13105658673342563 11 0.13767560626541678 12 0.1287031575664954 13 0.12502592449316696 14 0.11531802917957988 15 0.1309094974104925 16 0.11781854766944322 17 0.12120160209690539 18 0.11002281355398692 19 0.09722604245880397 20 0.09193082683321101 21 0.09119538021854531 22 0.09957947162573416 23 0.08413509271775471 24 0.09251918412494356 25 0.0872239684993506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 679859.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.60700513698201 #Duplication Level Percentage of deduplicated Percentage of total 1 68.1639960512433 38.585596746299515 2 16.90831669586106 19.14258340120651 3 6.702012544969251 11.38142575683577 4 3.26368649774141 7.389900733725874 5 1.7013562816693502 4.815434188814676 6 1.0335684350711931 3.5104328228098503 7 0.6054087907219421 2.3989264968459727 8 0.41176164383217534 1.8646874790096086 9 0.27428323721537223 1.3973717356234767 >10 0.9085153513246201 7.775511338103505 >50 0.018497257118336406 0.6863484208628877 >100 0.007815648392969013 0.6748386577037493 >500 5.210432259781079E-4 0.20631540250387936 >1k 2.6052161298905393E-4 0.17062681965474513 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1157 0.1701823466336402 No Hit TATCAACGCAGAGTACTTTTTTTTT 829 0.12193704871157109 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.470893229331376E-4 2 0.0 0.0 0.0 0.0 1.470893229331376E-4 3 0.0 0.0 0.0 0.0 1.470893229331376E-4 4 0.0 0.0 0.0 0.0 1.470893229331376E-4 5 0.0 0.0 0.0 0.0 1.470893229331376E-4 6 0.0 0.0 0.0 0.0 1.470893229331376E-4 7 0.0 0.0 0.0 0.0 1.470893229331376E-4 8 0.0 0.0 0.0 0.0 1.470893229331376E-4 9 0.0 0.0 0.0 0.0 1.470893229331376E-4 10 0.0 0.0 0.0 1.470893229331376E-4 1.470893229331376E-4 11 0.0 0.0 0.0 1.470893229331376E-4 4.4126796879941283E-4 12 0.0 0.0 0.0 1.470893229331376E-4 0.0011767145834651008 13 0.0 0.0 0.0 1.470893229331376E-4 0.001470893229331376 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTCCGT 25 0.0060248775 19.002064 6 GTCTAGC 40 2.7652667E-4 16.619461 1 GCACCGT 50 0.0014968059 13.301444 6 AACAGTT 75 1.4740352E-5 12.668041 7 TATACTG 70 1.0880535E-4 12.2156105 5 GTATCAA 485 0.0 12.140283 1 TGCACCG 55 0.0030628964 12.092221 5 CTTACAC 55 0.0030679698 12.08955 3 TAAGACT 105 2.7123315E-7 11.761449 4 GTCCTAG 105 2.722736E-7 11.757985 1 TTAGGGT 65 8.01582E-4 11.691855 4 GCATAAG 65 8.0356543E-4 11.688412 1 GTCTTAT 65 8.0356543E-4 11.688412 1 GTTATAG 65 8.0356543E-4 11.688412 1 GGTATCA 195 0.0 11.688411 1 TTAGACT 75 2.071283E-4 11.399558 4 GTATTAG 75 2.076958E-4 11.396201 1 GCCCTAG 75 2.076958E-4 11.396201 1 CTAGTAC 70 0.0014909271 10.855923 3 GTCACAC 80 3.7686952E-4 10.686298 3 >>END_MODULE