FastQCFastQC Report
Thu 2 Feb 2017
SRR4062022_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062022_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679859
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG300.00574153129.335152
TAGGACG801.3082172E-724.749714
GTATCAA7650.024.1654641
GTCCTAA1500.023.4750231
GGTATCA3400.023.3024141
GGTACGA602.871214E-421.99974442
GTATACA708.0989115E-418.863861
TATCAAC10000.018.4811422
GTATTAG600.00739364518.3398651
GTCTAGG1203.1451236E-718.3398651
GTGTAAG1801.4551915E-1118.3398631
TACGACC600.007409348618.3331244
GTACGAC600.007409348618.3331243
TTACACC1456.2937033E-918.2066844
TAGGACA1852.1827873E-1117.837634
ATCAACG10200.017.6873683
GTAGGAC2250.017.601093
CTATGAT750.00129018417.59979432
GGACCGA750.00129018417.5997946
TCAACGC10200.017.4703854
Produced by FastQC (version 0.11.5)