##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679859
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27394945128328	32.0	32.0	32.0	32.0	32.0
2	31.459237871382154	32.0	32.0	32.0	32.0	32.0
3	31.580364457924365	32.0	32.0	32.0	32.0	32.0
4	31.654282726271184	32.0	32.0	32.0	32.0	32.0
5	31.62263351665566	32.0	32.0	32.0	32.0	32.0
6	35.274073006314545	36.0	36.0	36.0	36.0	36.0
7	35.285978416112755	36.0	36.0	36.0	36.0	36.0
8	35.22962702560384	36.0	36.0	36.0	36.0	36.0
9	35.32782679938046	36.0	36.0	36.0	36.0	36.0
10	35.206704625517936	36.0	36.0	36.0	36.0	36.0
11	35.31459170210294	36.0	36.0	36.0	36.0	36.0
12	35.255941305476576	36.0	36.0	36.0	36.0	36.0
13	35.286719746300335	36.0	36.0	36.0	36.0	36.0
14	35.25273034555695	36.0	36.0	36.0	36.0	36.0
15	35.22061633368095	36.0	36.0	36.0	36.0	36.0
16	35.2411691247744	36.0	36.0	36.0	36.0	36.0
17	35.2228153190588	36.0	36.0	36.0	36.0	36.0
18	35.215418197008496	36.0	36.0	36.0	36.0	36.0
19	35.2088991982161	36.0	36.0	36.0	36.0	36.0
20	35.2072444433331	36.0	36.0	36.0	36.0	36.0
21	35.201337336124105	36.0	36.0	36.0	36.0	36.0
22	35.18188330227297	36.0	36.0	36.0	36.0	36.0
23	35.15881087107768	36.0	36.0	36.0	36.0	36.0
24	35.14483738539903	36.0	36.0	36.0	36.0	36.0
25	35.133871876374364	36.0	36.0	36.0	36.0	36.0
26	35.09148514618472	36.0	36.0	36.0	36.0	36.0
27	35.06984242320834	36.0	36.0	36.0	36.0	36.0
28	35.06277331034818	36.0	36.0	36.0	36.0	36.0
29	35.03210812830307	36.0	36.0	36.0	36.0	36.0
30	35.01186716657425	36.0	36.0	36.0	36.0	36.0
31	35.00943430917293	36.0	36.0	36.0	36.0	36.0
32	34.98665458573028	36.0	36.0	36.0	36.0	36.0
33	34.96356744560269	36.0	36.0	36.0	36.0	36.0
34	34.954783271237126	36.0	36.0	36.0	36.0	36.0
35	34.94095099130849	36.0	36.0	36.0	36.0	36.0
36	34.91635471472761	36.0	36.0	36.0	36.0	36.0
37	34.896602089550925	36.0	36.0	36.0	36.0	36.0
38	34.89531505797525	36.0	36.0	36.0	36.0	36.0
39	34.86655321176891	36.0	36.0	36.0	32.0	36.0
40	34.85702623632253	36.0	36.0	36.0	32.0	36.0
41	34.830406010658095	36.0	36.0	36.0	32.0	36.0
42	34.81151827070025	36.0	36.0	36.0	32.0	36.0
43	34.79746241500076	36.0	36.0	36.0	32.0	36.0
44	34.76732822541145	36.0	36.0	36.0	32.0	36.0
45	34.75034823397205	36.0	36.0	36.0	32.0	36.0
46	34.722489516208505	36.0	36.0	36.0	32.0	36.0
47	34.69909937207568	36.0	36.0	36.0	32.0	36.0
48	34.67080968259595	36.0	36.0	36.0	32.0	36.0
49	34.65423271590139	36.0	36.0	36.0	32.0	36.0
50	34.23969676065184	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	4.0
21	14.0
22	30.0
23	121.0
24	297.0
25	728.0
26	1566.0
27	2924.0
28	5025.0
29	8124.0
30	12290.0
31	18144.0
32	27866.0
33	46975.0
34	107416.0
35	448334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69431606331213	17.55915464151132	12.360196993306523	26.386332301870024
2	16.13513907091889	18.744750549031895	38.07714541659496	27.042964963454256
3	20.011296509849146	21.23361417711986	27.718958132310373	31.036131180720623
4	13.323056692637739	15.033999696995995	34.1170742756954	37.525869334670865
5	14.986342756365659	36.0217339183566	33.287490494352504	15.704432830925235
6	36.63818527076938	33.615793863139274	15.526307660853206	14.219713205238143
7	31.771882110849454	29.464050634028528	18.577822754424076	20.18624450069794
8	27.906963061458335	34.51215619709381	17.858408875958105	19.722471865489755
9	26.25059019590827	15.722083549677212	18.37954634711021	39.64777990730431
10	16.78862823026539	27.13753881319509	30.806976152408073	25.266856804131443
11	37.096809779674906	21.54873289902759	21.217929011750964	20.136528309546538
12	24.603925225671794	23.304096878911658	28.391475291200084	23.70050260421646
13	30.126099676550577	19.592444904016862	24.20707823239819	26.07437718703437
14	22.764279063747043	19.28752873757647	25.32863431976336	32.61955787891313
15	25.04622281973174	27.187696272315286	21.86041517432291	25.905665733630062
16	25.33080729335208	26.189376511358354	23.696775180480458	24.783041014809108
17	24.258118227455988	26.292510652944213	25.013863168686452	24.43550795091335
18	24.362698736061446	25.70768350496206	25.66032074297759	24.269297015998905
19	25.467377011081727	25.160254053052256	25.358677841549266	24.013691094316755
20	25.33006989715469	25.045009531430185	25.48657362735638	24.138346944058743
21	25.363641578621447	24.468603048573307	25.483666466134892	24.684088906670354
22	25.02283561738537	24.935317470240154	25.289949827831947	24.751897084542527
23	24.648199111874668	24.911930267893784	25.423506933055233	25.016363687176312
24	24.574799038030168	24.93840598362886	25.461605783586204	25.025189194754766
25	24.984224623707632	24.748292655661412	25.453735123709837	24.813747596921118
26	24.50298298463204	25.205190510931725	25.53526040808642	24.756566096349815
27	24.739173090811743	25.27090255744958	25.250015812148728	24.739908539589944
28	24.88159187587887	25.24078770085254	25.08840159328795	24.78921882998064
29	24.567003258047674	25.367762243419552	25.300983297909113	24.76425120062366
30	24.960469279297964	25.177281492562344	25.160513112431985	24.701736115707707
31	24.93550085753706	24.91902673221015	25.283958026282114	24.861514383970675
32	24.663087445760095	25.343531661395897	25.19570493491211	24.797675957931897
33	24.69169941870043	25.127821185662846	25.378609587912738	24.80186980772399
34	24.90777350231228	25.026329414810373	25.328014497358232	24.737882585519117
35	24.652648052975767	25.213206287195266	25.48929335243536	24.644852307393602
36	24.6800793109129	25.190481513732323	25.446123885058014	24.68331529029677
37	24.96197668295467	24.921085069265686	25.25557258555614	24.861365662223502
38	24.574654450408097	25.35628711247479	25.151097506983067	24.917960930134043
39	24.86618470114951	25.060490839958522	25.256562061027722	24.81676239786425
40	25.11050085385352	24.95179147440866	25.141242522346545	24.79646514939127
41	24.80264057859737	24.85868227008565	25.48381924862948	24.8548579026875
42	24.733132990013722	25.27782558363598	25.25017173335216	24.738869692998133
43	24.821427520755005	24.877029165857646	25.56145894009732	24.740084373290028
44	24.572729719830726	25.222883653335572	25.510010458358522	24.69437616847518
45	24.781898173548893	25.093293598669103	25.333790301584337	24.791017926197668
46	24.902293330352244	25.114401749820914	25.12337443608138	24.85993048374546
47	24.785356748336763	25.201478563622214	25.42579308052229	24.58737160751873
48	24.7126867921975	25.304155579384936	25.329455910495668	24.6537017179219
49	24.863313627745313	25.210159434338326	25.123080249646605	24.803446688269755
50	24.826946102499683	25.707733810304102	24.964035972852674	24.50128411434354
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	160.0
1	95.5
2	31.0
3	53.5
4	76.0
5	93.0
6	110.0
7	91.5
8	73.0
9	102.5
10	132.0
11	249.5
12	367.0
13	674.5
14	982.0
15	1153.5
16	1325.0
17	1336.0
18	1347.0
19	1283.0
20	1219.0
21	1419.5
22	1620.0
23	2054.5
24	2489.0
25	3198.0
26	3907.0
27	4811.0
28	5715.0
29	6967.5
30	8220.0
31	9734.0
32	11248.0
33	13191.0
34	15134.0
35	17478.5
36	19823.0
37	22231.0
38	24639.0
39	26829.0
40	29019.0
41	31638.5
42	34258.0
43	36206.0
44	38154.0
45	41341.5
46	44529.0
47	46787.5
48	49046.0
49	50544.0
50	52042.0
51	52967.0
52	53892.0
53	53503.5
54	53115.0
55	51667.0
56	50219.0
57	47191.5
58	44164.0
59	40803.0
60	37442.0
61	33636.0
62	29830.0
63	25757.5
64	21685.0
65	18785.0
66	15885.0
67	12990.0
68	10095.0
69	8331.5
70	6568.0
71	5304.0
72	4040.0
73	3392.0
74	2744.0
75	2236.0
76	1728.0
77	1435.5
78	1143.0
79	991.5
80	840.0
81	651.5
82	463.0
83	318.0
84	173.0
85	127.0
86	81.0
87	60.5
88	40.0
89	25.5
90	11.0
91	12.0
92	13.0
93	10.0
94	7.0
95	6.5
96	6.0
97	7.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0355956161498193
2	0.003824322396261578
3	4.4126796879941283E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010296252605319633
17	0.0
18	0.0
19	1.470893229331376E-4
20	4.4126796879941283E-4
21	0.0
22	0.0
23	0.0
24	5.883572917325504E-4
25	2.941786458662752E-4
26	4.4126796879941283E-4
27	2.941786458662752E-4
28	0.0010296252605319633
29	5.883572917325504E-4
30	0.0011767145834651008
31	7.35446614665688E-4
32	0.0013238039063982385
33	4.4126796879941283E-4
34	0.0016179825522645139
35	1.470893229331376E-4
36	4.4126796879941283E-4
37	0.001912161198130789
38	0.0
39	5.883572917325504E-4
40	0.0
41	0.0011767145834651008
42	0.003824322396261578
43	0.003383054427462165
44	0.002941786458662752
45	0.0023534291669302016
46	0.002647607812796477
47	0.001470893229331376
48	0.003824322396261578
49	0.002647607812796477
50	0.001912161198130789
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	679859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.29475969836763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8462472437208	38.8723443226502
2	17.16129656826952	19.665047259828462
3	6.761728445828485	11.622348193480796
4	3.2816175755453285	7.5207796165123755
5	1.7730302804911044	5.07926719293336
6	1.0207776117426188	3.509112478216017
7	0.6078039971236232	2.4376788771233726
8	0.4076562631064643	1.868525410737552
9	0.2790734267211487	1.4390500429969404
>10	0.8444999996840884	7.126538253459263
>50	0.013169839816856396	0.5116218979085178
>100	0.0030987479499634944	0.34768645415310795
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.941786458662752E-4
2	0.0	0.0	0.0	0.0	2.941786458662752E-4
3	0.0	0.0	0.0	0.0	2.941786458662752E-4
4	0.0	0.0	0.0	0.0	2.941786458662752E-4
5	0.0	0.0	0.0	0.0	2.941786458662752E-4
6	0.0	0.0	0.0	0.0	5.883572917325504E-4
7	0.0	0.0	0.0	0.0	5.883572917325504E-4
8	0.0	0.0	0.0	0.0	5.883572917325504E-4
9	0.0	0.0	0.0	0.0	5.883572917325504E-4
10	0.0	0.0	0.0	0.0	7.35446614665688E-4
11	0.0	0.0	0.0	0.0	7.35446614665688E-4
12	0.0	0.0	0.0	0.0	0.0016179825522645136
13	0.0	0.0	0.0	0.0	0.0019121611981307888
14	0.0	0.0	0.0	0.0	0.0019121611981307888
15	0.0	0.0	0.0	0.0	0.002941786458662752
16	0.0	0.0	0.0	0.0	0.002941786458662752
17	0.0	0.0	0.0	1.470893229331376E-4	0.002941786458662752
18	0.0	0.0	0.0	4.4126796879941283E-4	0.0030888757815958896
19	0.0	0.0	0.0	7.35446614665688E-4	0.0030888757815958896
20	0.0	0.0	0.0	8.825359375988257E-4	0.003383054427462165
21	0.0	0.0	0.0	0.0013238039063982385	0.003383054427462165
22	0.0	0.0	0.0	0.0019121611981307888	0.003383054427462165
23	0.0	0.0	0.0	0.0032359651045290273	0.003383054427462165
24	0.0	0.0	0.0	0.004118501042127853	0.003383054427462165
25	0.0	0.0	0.0	0.004706858333860403	0.003383054427462165
26	0.0	0.0	0.0	0.005883572917325504	0.0035301437503953027
27	0.0	0.0	0.0	0.00676610885492433	0.0038243223962615776
28	0.0	0.0	0.0	0.008384091407188843	0.0038243223962615776
29	0.0	0.0	0.0	0.010737520574119046	0.0038243223962615776
30	0.0	0.0	0.0	0.013238039063982385	0.0038243223962615776
31	0.0	0.0	0.0	0.018827433335441613	0.003971411719194715
32	0.0	0.0	0.0	0.026034810159165356	0.004118501042127853
33	0.0	0.0	0.0	0.032359651045290275	0.004118501042127853
34	0.0	0.0	0.0	0.0391257599002146	0.004118501042127853
35	0.0	0.0	0.0	0.04765694063033658	0.004118501042127853
36	0.0	0.0	0.0	0.064130944798848	0.004118501042127853
37	0.0	0.0	0.0	0.07913405573802804	0.004118501042127853
38	0.0	0.0	0.0	0.10046200756333298	0.004265590365060991
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	30	0.005741531	29.33515	2
TAGGACG	80	1.3082172E-7	24.74971	4
GTATCAA	765	0.0	24.165464	1
GTCCTAA	150	0.0	23.475023	1
GGTATCA	340	0.0	23.302414	1
GGTACGA	60	2.871214E-4	21.999744	42
GTATACA	70	8.0989115E-4	18.86386	1
TATCAAC	1000	0.0	18.481142	2
GTATTAG	60	0.007393645	18.339865	1
GTCTAGG	120	3.1451236E-7	18.339865	1
GTGTAAG	180	1.4551915E-11	18.339863	1
TACGACC	60	0.0074093486	18.33312	44
GTACGAC	60	0.0074093486	18.33312	43
TTACACC	145	6.2937033E-9	18.206684	4
TAGGACA	185	2.1827873E-11	17.83763	4
ATCAACG	1020	0.0	17.687368	3
GTAGGAC	225	0.0	17.60109	3
CTATGAT	75	0.001290184	17.599794	32
GGACCGA	75	0.001290184	17.599794	6
TCAACGC	1020	0.0	17.470385	4
>>END_MODULE