FastQCFastQC Report
Thu 2 Feb 2017
SRR4062020_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062020_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243266
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14240.5853674578444995No Hit
GTCCTACAGTGTGCATTTCTCATTT10080.41436123420453325No Hit
TATCAACGCAGAGTACTTTTTTTTT9530.39175223829059547No Hit
GTCCTACAGTGGACATTTCTAAATT9430.3876415117607886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA9360.38476400318992376No Hit
CTTTAGGACGTGAAATATGGCGAGG9360.38476400318992376No Hit
GTCCTAAAGTGTGTATTTCTCATTT9140.37572040482434865No Hit
TTGTAGAACAGTGTATATCAATGAG8660.35598891748127565No Hit
GATATACACTGTTCTACAAATCCCG8590.3531114089104108No Hit
GTGTATATCAATGAGTTACAATGAA8260.33954601136204815No Hit
CTGTAGGACGTGGAATATGGCAAGA7640.3140595068772455No Hit
GGTATCAACGCAGAGTACTTTTTTT6350.26103113464273675No Hit
GATATACACTGTTCTACAATGCCGG6040.24828788240033542No Hit
CTGTAGGACCTGGAATATGGCGAGA5850.24047750199370238No Hit
GTGTATATCAATGAGTTACAATGAG5240.21540207016188043No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5160.21211348893803492No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5130.21088027097909287No Hit
GTTCTACAGTGTGGTTTTTATCATT4790.19690380077774947No Hit
GTTCTACAAATCCCGTTTCCAACGA4530.18621591180025157No Hit
CTGTAGAACATATTAGATGAGTGAG4090.16812871506910132No Hit
GTACATGGGGTGGTATCAACGCAAA4000.16442906119227513No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3790.1557965354796807No Hit
ATTTAGAAATGTCCACTGTAGGACG3600.1479861550730476No Hit
CTGTAGGACATGGAATATGGCAAGA3420.14058684731939525No Hit
GAATATGGCAAGAAAACTGAAAATC3380.13894255670747246No Hit
CCATAGGGTCTTCTCGTCTTATTAT3320.13647612078958837No Hit
GTATCAACGCAGAGTACATGGGGTG3180.13072110364785872No Hit
CTGAAGGACCTGGAATATGGCGAGA3080.12661037711805184No Hit
GTAGAACAGTGTATATCAATGAGTT3000.12332179589420633No Hit
ATGTTCTACAGTGTGGTTTTTATCA2990.12291072324122565No Hit
GTCCTTCAGTGTGCATTTCTCATTT2960.12167750528228359No Hit
GTTCTACAATGCCGGTTTCCAACGT2890.11879999671141878No Hit
TTTCTAAATTTTCCACCTTTTTCAG2820.11592248814055396No Hit
GTAGAACATATTAGATGAGTGAGTT2730.11222283426372778No Hit
GAAATATGGCGAGGAAAACTGAAAA2500.10276816324517196No Hit
TATTAGATGAGTGAGTTACACTGAA2460.1011238726332492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACG250.006008336319.005156
TCTAGAC350.002162176416.2867723
ATAACCC350.002162176416.2867723
CCTACAC508.660976E-515.200993
CCAACGA704.398389E-714.92646819
AGGCATA456.719477E-414.7817835
TCCAACG1058.731149E-1114.47415318
TGGTCGG400.005259877514.25092710
TTCTATT400.00526707214.2479922
CCCGGCG400.00526707214.24799218
AATAACC400.00526707214.2479922
AAATGTC1051.3405952E-913.5751077
CTGTGCA852.665347E-713.4126379
AATACTA500.00149015413.3036055
TCTATGT500.0014924713.3008663
TAGGACC2050.012.9791274
TAGAAAT1251.0004442E-1012.9235014
ATTTAGA1251.0004442E-1012.9155211
GTTACAA2950.012.87954215
AATGTCC1207.239578E-1012.6674918
Produced by FastQC (version 0.11.5)