FastQCFastQC Report
Thu 2 Feb 2017
SRR4062020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243266
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG5240.21540207016188043No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.21416885220293833No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC4270.1755280228227537No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC3890.15990726200948757No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA3880.15949618935650686No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3770.1549743901737193No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA3310.13606504813660766No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG3290.1352429028306463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3280.1348318301776656No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA3250.13359861221872354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.1278435950769939No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC3070.12619930446507116No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3070.12619930446507116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.12578823181209048No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA2600.10687888977497884No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2500.10276816324517196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGAGC301.2981165E-436.66649628
CTTCCGC250.002346797435.2070731
GTCTTAC250.002346797435.2070731
GATCGAT250.002349166235.19983719
AGCCTAT250.002349166235.1998376
GGACCGT250.002349166235.1998376
GAGCATA353.2104563E-431.42842531
GACCTAG353.2104563E-431.4284257
TCTGCAC300.00573624329.33319915
GCGCGAT300.00573624329.33319928
TAGCGGA300.00573624329.3331996
AGAACCG300.00573624329.3331995
TAGGACC2950.029.084614
CGGATGG407.0160767E-427.49987214
AGCATAG407.0160767E-427.49987232
TGCTACA407.0160767E-427.4998722
AGGACCT4400.025.999885
GGACCTG3950.025.0631756
CTAGAAC450.001395047624.44432810
TCGGATG450.001395047624.44432813
Produced by FastQC (version 0.11.5)