##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062020_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243266
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.10934121496633	32.0	32.0	32.0	32.0	32.0
2	31.45363511547031	32.0	32.0	32.0	32.0	32.0
3	31.605033173563097	32.0	32.0	32.0	32.0	32.0
4	31.717695855565513	32.0	32.0	32.0	32.0	32.0
5	31.705486997771985	32.0	32.0	32.0	32.0	32.0
6	35.36698922167504	36.0	36.0	36.0	36.0	36.0
7	35.37038468178866	36.0	36.0	36.0	36.0	36.0
8	35.33066684205767	36.0	36.0	36.0	36.0	36.0
9	35.373858245706344	36.0	36.0	36.0	36.0	36.0
10	35.287438441870215	36.0	36.0	36.0	36.0	36.0
11	35.39539845272253	36.0	36.0	36.0	36.0	36.0
12	35.32947473136402	36.0	36.0	36.0	36.0	36.0
13	35.34958029482131	36.0	36.0	36.0	36.0	36.0
14	35.31586822655036	36.0	36.0	36.0	36.0	36.0
15	35.30981312637196	36.0	36.0	36.0	36.0	36.0
16	35.30654509878076	36.0	36.0	36.0	36.0	36.0
17	35.30975146547401	36.0	36.0	36.0	36.0	36.0
18	35.316188863219686	36.0	36.0	36.0	36.0	36.0
19	35.242080685340326	36.0	36.0	36.0	36.0	36.0
20	35.19250943411738	36.0	36.0	36.0	36.0	36.0
21	35.15240107536606	36.0	36.0	36.0	36.0	36.0
22	35.140340203727604	36.0	36.0	36.0	36.0	36.0
23	35.14154875732737	36.0	36.0	36.0	36.0	36.0
24	35.121422640237434	36.0	36.0	36.0	36.0	36.0
25	35.07024820566787	36.0	36.0	36.0	36.0	36.0
26	35.04963291212089	36.0	36.0	36.0	36.0	36.0
27	35.03653202667039	36.0	36.0	36.0	36.0	36.0
28	34.967171737932965	36.0	36.0	36.0	36.0	36.0
29	34.927544334185626	36.0	36.0	36.0	36.0	36.0
30	34.856609637187276	36.0	36.0	36.0	36.0	36.0
31	34.88030797563162	36.0	36.0	36.0	36.0	36.0
32	34.81982274547204	36.0	36.0	36.0	36.0	36.0
33	34.790722912367535	36.0	36.0	36.0	32.0	36.0
34	34.75310565389327	36.0	36.0	36.0	32.0	36.0
35	34.71706691440645	36.0	36.0	36.0	32.0	36.0
36	34.646678944036566	36.0	36.0	36.0	32.0	36.0
37	34.60655825310565	36.0	36.0	36.0	32.0	36.0
38	34.55399439296902	36.0	36.0	36.0	32.0	36.0
39	34.33927881413761	36.0	36.0	36.0	32.0	36.0
40	34.42586304703493	36.0	36.0	36.0	32.0	36.0
41	34.39495449425731	36.0	36.0	36.0	32.0	36.0
42	34.37625068854669	36.0	36.0	36.0	32.0	36.0
43	34.38153708286403	36.0	36.0	36.0	32.0	36.0
44	34.21824669292051	36.0	36.0	36.0	32.0	36.0
45	34.16986755239121	36.0	36.0	36.0	32.0	36.0
46	33.969227101197866	36.0	36.0	36.0	32.0	36.0
47	33.95206481793592	36.0	36.0	36.0	32.0	36.0
48	33.890975310976465	36.0	36.0	36.0	32.0	36.0
49	33.75296588919126	36.0	36.0	36.0	32.0	36.0
50	33.43467644471484	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	2.0
21	3.0
22	11.0
23	43.0
24	96.0
25	273.0
26	694.0
27	1465.0
28	2632.0
29	3909.0
30	5770.0
31	8313.0
32	11877.0
33	18743.0
34	40200.0
35	149231.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.161763617373	21.537778289511557	13.282644274658487	24.01781381845696
2	11.961325171936085	22.3354531589787	45.5045856473964	20.198636021688817
3	18.839614575111813	27.75009865824783	31.00129900026309	22.40898776637727
4	10.133351968626936	18.259847245402153	40.828146966694895	30.77865381927602
5	10.489751958761191	40.46886946798977	36.448989994491626	12.59238857875741
6	28.06146358307367	41.559445216347534	18.4440077939375	11.93508340664129
7	24.286994483404996	33.16246413391103	23.086662336701387	19.463879045982587
8	22.20820007728166	41.520804386967356	20.502248567411804	15.768746968339183
9	25.39236884727007	14.715989904055643	21.2984962962354	38.5931449524389
10	14.831090246890236	29.394983269343022	34.248107010433024	21.52581947333372
11	33.61258868892488	23.03404503711986	26.480478159709946	16.872888114245313
12	22.751638124522128	25.76356745291163	34.550656483026806	16.934137939539433
13	29.13682964327115	23.204640188106847	27.620382626425393	20.038147542196608
14	19.304382856626077	24.145585490779638	28.638198515205577	27.911833137388705
15	21.96361184875815	34.43884472141606	23.957725288367467	19.63981814145832
16	19.7679867796861	28.683065994688857	31.50553723968396	20.043409985941086
17	17.59555383818536	29.94088775250138	30.912252431494743	21.55130597781852
18	17.603364218591995	29.314824102011787	35.164387953926976	17.917423725469238
19	22.99211562651583	25.299466427696434	28.661629656425475	23.04678828936226
20	22.63704190902925	29.099541652107785	29.844408361252135	18.419008077610837
21	22.532947473136403	26.80358126495277	27.83044075209853	22.833030509812303
22	21.37125615581298	30.344150025075432	29.441434479129843	18.84315933998175
23	20.84590530530366	30.02433550105646	29.65149260480297	19.478266588836913
24	22.75707561712536	27.80753499270343	30.44909871950342	18.986290670667792
25	21.977588318959494	28.619289173168465	30.60600330502413	18.797119202847913
26	17.89331754801368	30.715190081557488	32.71384175217048	18.677650618258355
27	18.26485519906275	29.72067498407087	32.43006597743202	19.584403839434362
28	18.29385600710345	30.955924065410954	29.69719890488445	21.053021022601147
29	19.25693590887229	26.993418645663336	29.369858959233426	24.37978648623095
30	19.2791341058854	29.04634079165348	29.749283697068606	21.92524140539252
31	22.290278422941427	27.652787312497175	29.77353728269404	20.28339698186736
32	18.80089614206729	30.359484512774138	30.8375639734446	20.002055371713976
33	19.35782230151357	30.43869673526099	31.554347915450577	18.649133047774864
34	19.71101473701519	29.335087250529256	32.17766633095595	18.7762316814996
35	19.16428586109798	30.86305058269788	30.61023986187902	19.362423694325116
36	18.483470768623647	30.5854496723751	30.873200529461574	20.05787902953968
37	19.09199659622049	29.62315885537637	29.186587135522224	22.09825741288092
38	20.35097383111491	31.87827316599936	28.85524487597938	18.91550812690635
39	21.04445339669333	27.466230381557637	30.01446975738492	21.474846464364113
40	23.319740530941438	28.541596441755118	30.058043458600874	18.080619568702573
41	22.070442568095306	27.101643495490457	32.288643520155226	18.53927041625901
42	19.96957924811412	32.024418819757045	29.95128568785842	18.054716244270416
43	18.045409511907355	31.775558780364477	31.36158649636387	18.8174452113643
44	17.89985159850201	31.012213319959386	30.072885278653615	21.01504980288499
45	18.789670141743677	31.008895977899826	30.01981451639425	20.181619363962245
46	20.168053408754563	28.761469398493766	28.266106488637483	22.804370704114184
47	21.71115916172686	30.122666096636546	28.546176550386004	19.619998191250584
48	18.97959351465123	30.059690202913803	28.26651757818923	22.694198704245732
49	21.093827079290953	29.02210017430197	29.37070411418423	20.513368632222846
50	19.75376646866586	31.438965736953527	27.071300844757772	21.73596694962284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	595.0
1	317.0
2	39.0
3	119.5
4	200.0
5	186.0
6	172.0
7	116.0
8	60.0
9	103.0
10	146.0
11	252.0
12	358.0
13	659.5
14	961.0
15	1230.0
16	1499.0
17	1586.0
18	1673.0
19	1708.5
20	1744.0
21	1909.5
22	2075.0
23	2149.0
24	2223.0
25	2996.0
26	3769.0
27	5830.5
28	7892.0
29	9277.5
30	10663.0
31	12072.5
32	13482.0
33	15923.0
34	18364.0
35	21113.5
36	23863.0
37	23902.0
38	23941.0
39	22280.5
40	20620.0
41	19003.5
42	17387.0
43	15020.0
44	12653.0
45	12151.5
46	11650.0
47	10984.5
48	10319.0
49	9738.0
50	9157.0
51	9143.0
52	9129.0
53	8639.0
54	8149.0
55	7875.5
56	7602.0
57	6985.5
58	6369.0
59	5749.5
60	5130.0
61	4437.0
62	3744.0
63	3170.0
64	2596.0
65	2213.5
66	1831.0
67	1511.5
68	1192.0
69	999.0
70	806.0
71	687.5
72	569.0
73	450.0
74	331.0
75	249.5
76	168.0
77	128.5
78	89.0
79	65.5
80	42.0
81	25.5
82	9.0
83	6.0
84	3.0
85	1.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034530102850377775
2	0.0028775085708648146
3	8.221453059613756E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0016442906119227513
17	0.0
18	0.0
19	0.0
20	4.110726529806878E-4
21	0.0
22	0.0
23	0.0
24	4.110726529806878E-4
25	0.0
26	8.221453059613756E-4
27	4.110726529806878E-4
28	0.0016442906119227513
29	0.0012332179589420633
30	0.0012332179589420633
31	0.0012332179589420633
32	4.110726529806878E-4
33	0.0
34	4.110726529806878E-4
35	4.110726529806878E-4
36	0.0
37	0.0028775085708648146
38	0.0
39	0.0
40	0.0
41	0.0016442906119227513
42	0.004521799182787566
43	0.005343944488748941
44	0.0028775085708648146
45	0.004110726529806878
46	0.004110726529806878
47	0.0016442906119227513
48	0.004110726529806878
49	0.004110726529806878
50	4.110726529806878E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	243266.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.269225372060774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.85925506997533	28.064097250867377
2	17.06859178917693	14.770894898192052
3	7.08348894651458	9.194912389417219
4	3.4034975505663754	5.890668102748532
5	1.9935122767611775	4.312886599257469
6	1.2771334618350334	3.315634535420414
7	0.8168808238391128	2.474206032816647
8	0.5867588520066929	2.03108808052234
9	0.43832572571699785	1.7069413151178787
>10	2.251400257235917	17.98107449602726
>50	0.1484330257180691	4.356579644500846
>100	0.07072983104686514	5.450573820590996
>500	0.001992389606953948	0.45044283452096406
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	524	0.21540207016188043	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	521	0.21416885220293833	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	427	0.1755280228227537	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	389	0.15990726200948757	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	388	0.15949618935650686	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	377	0.1549743901737193	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	331	0.13606504813660766	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	329	0.1352429028306463	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	328	0.1348318301776656	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	325	0.13359861221872354	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	311	0.1278435950769939	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	307	0.12619930446507116	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	307	0.12619930446507116	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	306	0.12578823181209048	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	260	0.10687888977497884	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	250	0.10276816324517196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.002055363264903439	0.0
25	0.0	0.0	0.0	0.0024664359178841267	0.0
26	0.0	0.0	0.0	0.004521799182787566	0.0
27	0.0	0.0	0.0	0.006166089794710317	0.0
28	0.0	0.0	0.0	0.009043598365575133	0.0
29	0.0	0.0	0.0	0.012332179589420634	0.0
30	0.0	0.0	0.0	0.022197923260957143	0.0
31	0.0	0.0	0.0	0.038640829380184656	0.0
32	0.0	0.0	0.0	0.050150863663643915	0.0
33	0.0	0.0	0.0	0.062483043253064544	0.0
34	0.0	0.0	0.0	0.07522629549546588	0.0
35	0.0	0.0	0.0	0.08961383834978995	0.0
36	0.0	0.0	0.0	0.10852318038690158	0.0
37	0.0	0.0	0.0	0.13359861221872354	0.0
38	0.0	0.0	0.0	0.17018407833400476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGAGC	30	1.2981165E-4	36.666496	28
CTTCCGC	25	0.0023467974	35.207073	1
GTCTTAC	25	0.0023467974	35.207073	1
GATCGAT	25	0.0023491662	35.199837	19
AGCCTAT	25	0.0023491662	35.199837	6
GGACCGT	25	0.0023491662	35.199837	6
GAGCATA	35	3.2104563E-4	31.428425	31
GACCTAG	35	3.2104563E-4	31.428425	7
TCTGCAC	30	0.005736243	29.333199	15
GCGCGAT	30	0.005736243	29.333199	28
TAGCGGA	30	0.005736243	29.333199	6
AGAACCG	30	0.005736243	29.333199	5
TAGGACC	295	0.0	29.08461	4
CGGATGG	40	7.0160767E-4	27.499872	14
AGCATAG	40	7.0160767E-4	27.499872	32
TGCTACA	40	7.0160767E-4	27.499872	2
AGGACCT	440	0.0	25.99988	5
GGACCTG	395	0.0	25.063175	6
CTAGAAC	45	0.0013950476	24.444328	10
TCGGATG	45	0.0013950476	24.444328	13
>>END_MODULE