FastQCFastQC Report
Thu 2 Feb 2017
SRR4062019_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062019_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151782
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34260.15921687234115722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG957.3785486E-813.00187
GTACTAA905.432739E-712.6619371
GTATCAA22000.012.4748861
GGTATCA9100.012.4184371
GTCCTAG1650.012.0863931
GTTTTCG1459.822543E-1111.7930915
GTATAAG1950.011.6879421
TAATTCA2150.011.490239
CGGACAT2200.011.2277815
CCGACCA2650.011.1150049
AGAACCG1803.6379788E-1211.0838365
CGTCGTA2200.010.79745110
TTTTCGG1605.456968E-1010.68674116
GATAATT2250.010.5570167
GTCCTAA1355.7212674E-810.5516141
CCGTCAA2800.010.516376518
CGACCAT3000.010.45193310
TTCGGAA1752.3646862E-1010.31283418
TTTAGAC1201.5265468E-610.2904573
CAAGACG3050.010.2779584
Produced by FastQC (version 0.11.5)