FastQCFastQC Report
Thu 2 Feb 2017
SRR4062019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151782
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23000.028.0343761
GGTATCA10350.021.9001961
TCAACGC33250.018.9889134
ATCAACG33400.018.9036333
CGGATCG2100.018.85658526
CAACGCA34400.018.6099155
TATCAAC35200.018.2511562
AACGCAG35550.017.884146
ATACCGT5550.017.044546
CAATACG3500.016.34427338
GTCCTAT3750.015.8446861
ACCGTCG4450.015.81975658
CGCAATA3400.015.52967536
AACGCCG3400.015.5289535
ATACGAA3550.015.494340
GGCGTTA4850.015.42401642
GATATAC3150.015.3696241
CGTCGTA4600.015.30389510
GTATAGC3500.015.0901771
GATCTAA1756.39302E-815.0901771