##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151782
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28892285556808	32.0	32.0	32.0	32.0	32.0
2	31.441763617318113	32.0	32.0	32.0	32.0	32.0
3	31.565564727281853	32.0	32.0	32.0	32.0	32.0
4	31.64302657053549	32.0	32.0	32.0	32.0	32.0
5	31.61734599508686	32.0	32.0	32.0	32.0	32.0
6	35.25781793880607	36.0	36.0	36.0	36.0	36.0
7	35.26764653668448	36.0	36.0	36.0	36.0	36.0
8	35.21892459366237	36.0	36.0	36.0	36.0	36.0
9	35.29985472506044	36.0	36.0	36.0	36.0	36.0
10	35.18091609651907	36.0	36.0	36.0	36.0	36.0
11	35.30259013227176	36.0	36.0	36.0	36.0	36.0
12	35.2282271159439	36.0	36.0	36.0	36.0	36.0
13	35.2672468679448	36.0	36.0	36.0	36.0	36.0
14	35.22828985464141	36.0	36.0	36.0	36.0	36.0
15	35.19879569584651	36.0	36.0	36.0	36.0	36.0
16	35.21584760909795	36.0	36.0	36.0	36.0	36.0
17	35.19547240380299	36.0	36.0	36.0	36.0	36.0
18	35.190075016892976	36.0	36.0	36.0	36.0	36.0
19	35.180588925829845	36.0	36.0	36.0	36.0	36.0
20	35.18335639948656	36.0	36.0	36.0	36.0	36.0
21	35.17486715661717	36.0	36.0	36.0	36.0	36.0
22	35.164996732940416	36.0	36.0	36.0	36.0	36.0
23	35.12910043861321	36.0	36.0	36.0	36.0	36.0
24	35.11418396473249	36.0	36.0	36.0	36.0	36.0
25	35.103549987870515	36.0	36.0	36.0	36.0	36.0
26	35.05564132426054	36.0	36.0	36.0	36.0	36.0
27	35.04304571745651	36.0	36.0	36.0	36.0	36.0
28	35.02167552289219	36.0	36.0	36.0	36.0	36.0
29	35.0069658543477	36.0	36.0	36.0	36.0	36.0
30	34.98532565101855	36.0	36.0	36.0	36.0	36.0
31	34.99336410472808	36.0	36.0	36.0	36.0	36.0
32	34.962592400159494	36.0	36.0	36.0	36.0	36.0
33	34.94501534077337	36.0	36.0	36.0	36.0	36.0
34	34.93575882687001	36.0	36.0	36.0	36.0	36.0
35	34.924088499671434	36.0	36.0	36.0	36.0	36.0
36	34.8955953716501	36.0	36.0	36.0	32.0	36.0
37	34.89148575459782	36.0	36.0	36.0	32.0	36.0
38	34.87814797223882	36.0	36.0	36.0	32.0	36.0
39	34.856822391859396	36.0	36.0	36.0	32.0	36.0
40	34.84807104065375	36.0	36.0	36.0	32.0	36.0
41	34.81976055195182	36.0	36.0	36.0	32.0	36.0
42	34.79693296068096	36.0	36.0	36.0	32.0	36.0
43	34.78940524644225	36.0	36.0	36.0	32.0	36.0
44	34.7505026066767	36.0	36.0	36.0	32.0	36.0
45	34.734838845199	36.0	36.0	36.0	32.0	36.0
46	34.72296961309278	36.0	36.0	36.0	32.0	36.0
47	34.697205850778566	36.0	36.0	36.0	32.0	36.0
48	34.67561119109649	36.0	36.0	36.0	32.0	36.0
49	34.658549518492116	36.0	36.0	36.0	32.0	36.0
50	34.24597752002759	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	4.0
20	26.0
21	56.0
22	151.0
23	431.0
24	996.0
25	2361.0
26	4976.0
27	9476.0
28	16458.0
29	26183.0
30	38834.0
31	58120.0
32	88967.0
33	152423.0
34	350069.0
35	1402250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.451159277122436	17.237631471904237	12.18339241094776	26.12781684002557
2	16.252664199754797	19.139109104615095	37.04536645353069	27.562860242099426
3	19.197110109458368	22.379688973346216	27.84428869720236	30.57891221999306
4	13.026796419708333	15.481368913178855	34.32479156791122	37.16704309920159
5	15.236355372596003	35.92275422080593	32.76388256983401	16.077007836764057
6	36.30902615928495	33.98978240795174	15.793025213091122	13.908166219672188
7	31.589910130301302	29.647241216814713	19.25371622218236	19.509132430701623
8	28.618094212146026	33.3396226941205	18.15183880151428	19.890444292219193
9	27.033268239998286	14.539298125925395	17.956372903946587	40.47106073012973
10	16.372391056524805	26.896975110385302	30.69977846258518	26.030855370504714
11	37.85188276507564	21.123143515467646	21.305968727315314	19.719004992141397
12	24.947136838211307	23.024451361708575	28.1845930489241	23.843818751156018
13	29.854650703463452	19.311528770107753	24.669413537244942	26.164406989183846
14	23.70709486369902	19.038359833849338	24.596311336371436	32.65823396608021
15	25.482925314925026	26.85109365168033	21.640993372005155	26.024987661389492
16	26.156478692647774	25.752857300202763	23.021244380786225	25.06941962636324
17	24.675873299432748	25.77775072010083	24.441555882519697	25.10482009794672
18	25.23091336897203	25.20563198578294	25.120446736912356	24.443007908332678
19	25.925070476470204	24.847173180182754	24.746419479296694	24.481336864050355
20	26.158171741933984	24.343809180792604	24.446515086382174	25.051503990891238
21	26.60701688182167	24.232148052172573	24.33652665558128	24.824308410424475
22	26.282773998481257	24.02394852266633	24.5757702220764	25.117507256776012
23	24.959835596652262	24.246891296093796	25.104924711204347	25.688348396049598
24	25.109875223015216	24.69319287150652	24.79576011997626	25.401171785502
25	25.42082620938303	24.226186797064198	24.86956141871447	25.483425574838307
26	25.091761665977312	24.95870841214442	25.036225990696032	24.913303931182238
27	25.574177933349816	24.659582892611297	24.567751601935523	25.19848757210336
28	25.393167255023343	24.61315316420635	24.81484889550674	25.17883068526357
29	25.195501570100564	24.710134386852147	24.95430512419293	25.140058918854358
30	25.214893454858434	24.778274940932185	24.930661540949657	25.076170063259724
31	25.488875690476785	24.61666513304836	24.479847790706643	25.41461138576821
32	25.47965386474328	24.642943450942482	24.288071160352455	25.58933152396178
33	25.130241130822977	24.506570845133652	24.777602522193273	25.5855855018501
34	25.604410516756932	24.654074838992717	24.927942908290213	24.81357173596014
35	25.67648707255116	24.486726790588992	25.000255602576104	24.83653053428374
36	25.113022916883544	24.787384932260608	24.778973276028733	25.320618874827122
37	25.668863486274173	24.641140431472657	24.59466656628312	25.095329515970043
38	25.258274304739047	24.609184387637782	24.76491577678408	25.36762553083909
39	25.75885377817745	24.460152479476154	24.537159029912655	25.243834712433745
40	25.983917508333793	24.505979000651088	24.495197234291037	25.01490625672408
41	25.033844403578655	24.930068841352586	25.07079092035865	24.965295834710112
42	25.894228159472938	24.754332412505903	24.57972531333539	24.77171411468577
43	25.489287540084582	24.1952409722545	24.866105869777385	25.449365617883537
44	25.089208781546073	24.671351967333646	24.730375229935113	25.509064021185168
45	25.283329104193914	24.851910409166916	24.575017962224024	25.28974252441515
46	25.262497472969486	24.636491017713613	24.665304974822643	25.43570653449426
47	25.434249172770194	24.599676543852475	24.91434918392386	25.05172509945347
48	25.692419875883783	25.005054159677986	24.201640289762526	25.10088567467571
49	25.259664964923328	24.98267660912342	24.449659922433966	25.307998503519286
50	25.480400121203783	24.969234315542526	24.403229374443473	25.147136188810215
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	31.0
2	46.0
3	129.0
4	212.0
5	228.0
6	244.0
7	203.5
8	163.0
9	214.5
10	266.0
11	502.0
12	738.0
13	1349.5
14	1961.0
15	2580.0
16	3199.0
17	3108.5
18	3018.0
19	3024.5
20	3031.0
21	3779.0
22	4527.0
23	5710.5
24	6894.0
25	8686.5
26	10479.0
27	13646.5
28	16814.0
29	20170.0
30	23526.0
31	27047.5
32	30569.0
33	35316.5
34	40064.0
35	46668.0
36	53272.0
37	61252.5
38	69233.0
39	76750.0
40	84267.0
41	92387.0
42	100507.0
43	107481.5
44	114456.0
45	124360.5
46	134265.0
47	144259.5
48	154254.0
49	163168.0
50	172082.0
51	170531.0
52	168980.0
53	168434.0
54	167888.0
55	168155.5
56	168423.0
57	163234.5
58	158046.0
59	145042.5
60	132039.0
61	116566.5
62	101094.0
63	87582.5
64	74071.0
65	62440.0
66	50809.0
67	43336.5
68	35864.0
69	30820.5
70	25777.0
71	20118.0
72	14459.0
73	12500.0
74	10541.0
75	8058.5
76	5576.0
77	4793.0
78	4010.0
79	3387.5
80	2765.0
81	2136.0
82	1507.0
83	1234.0
84	961.0
85	726.0
86	491.0
87	342.5
88	194.0
89	125.5
90	57.0
91	48.5
92	40.0
93	30.5
94	21.0
95	22.0
96	23.0
97	28.0
98	33.0
99	21.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03053283278696448
2	0.004833203363537756
3	2.3236554632393057E-4
4	9.294621852957223E-5
5	4.6473109264786116E-5
6	1.3941932779435834E-4
7	0.0
8	0.0
9	0.0
10	4.6473109264786116E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.041504204422195E-4
17	0.0
18	4.6473109264786116E-5
19	0.0
20	3.253117648535028E-4
21	0.0
22	0.0
23	4.6473109264786116E-5
24	0.0010688815130900807
25	1.3941932779435834E-4
26	5.576773111774333E-4
27	4.6473109264786114E-4
28	0.001208300840884439
29	4.1825798338307506E-4
30	8.365159667661501E-4
31	3.7178487411828893E-4
32	0.0010224084038252944
33	1.8589243705914447E-4
34	7.435697482365779E-4
35	1.8589243705914447E-4
36	2.7883865558871667E-4
37	0.0016265588242675142
38	0.0
39	7.90042857501364E-4
40	4.6473109264786116E-5
41	0.0010688815130900807
42	0.004368472270889895
43	0.003810794959712461
44	0.004089633615301178
45	0.002044816807650589
46	0.0021842361354449473
47	0.0010224084038252944
48	0.004136106724565964
49	0.00311369832074067
50	0.001580085715002728
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2151782.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.91456039505208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43417522379417	39.591712751640884
2	15.045705793922126	16.22365227425199
3	5.113163925936283	8.2702195588408
4	2.303639773006867	4.967989026808913
5	1.1616160433989273	3.1314009163846452
6	0.7042133842270308	2.2780413020947363
7	0.4483299701699795	1.6920059277548885
8	0.311829574627731	1.344972354738416
9	0.22768559497499474	1.104801188522645
>10	1.119138931833328	10.803346551161393
>50	0.07438486453454381	2.789968047967099
>100	0.05038635620463423	5.281518927883199
>500	0.0043413358859835235	1.5654014114045858
>1k	0.001389227483514678	0.9549697605458337
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3941932779435836E-4
2	0.0	0.0	0.0	0.0	1.3941932779435836E-4
3	0.0	0.0	0.0	0.0	1.3941932779435836E-4
4	0.0	0.0	0.0	0.0	1.3941932779435836E-4
5	0.0	4.6473109264786116E-5	0.0	0.0	1.3941932779435836E-4
6	0.0	4.6473109264786116E-5	0.0	0.0	2.323655463239306E-4
7	0.0	4.6473109264786116E-5	0.0	4.6473109264786116E-5	2.323655463239306E-4
8	0.0	4.6473109264786116E-5	0.0	4.6473109264786116E-5	2.323655463239306E-4
9	0.0	4.6473109264786116E-5	0.0	4.6473109264786116E-5	2.323655463239306E-4
10	0.0	4.6473109264786116E-5	0.0	4.6473109264786116E-5	2.323655463239306E-4
11	0.0	4.6473109264786116E-5	0.0	4.6473109264786116E-5	2.788386555887167E-4
12	0.0	4.6473109264786116E-5	0.0	9.294621852957223E-5	6.506235297070056E-4
13	0.0	4.6473109264786116E-5	0.0	9.294621852957223E-5	6.970966389717917E-4
14	0.0	4.6473109264786116E-5	0.0	9.294621852957223E-5	7.435697482365779E-4
15	0.0	4.6473109264786116E-5	0.0	1.3941932779435836E-4	0.0010688815130900807
16	0.0	4.6473109264786116E-5	0.0	1.8589243705914447E-4	0.0010688815130900807
17	0.0	4.6473109264786116E-5	0.0	2.788386555887167E-4	0.0010688815130900807
18	0.0	4.6473109264786116E-5	0.0	3.253117648535028E-4	0.001161827731619653
19	0.0	4.6473109264786116E-5	0.0	3.7178487411828893E-4	0.001161827731619653
20	0.0	9.294621852957223E-5	0.0	6.506235297070056E-4	0.0013941932779435835
21	0.0	9.294621852957223E-5	0.0	7.90042857501364E-4	0.001533612605737942
22	0.0	9.294621852957223E-5	0.0	0.0010688815130900807	0.001533612605737942
23	0.0	9.294621852957223E-5	0.0	0.001626558824267514	0.001580085715002728
24	0.0	9.294621852957223E-5	0.0	0.0019053974798562308	0.001626558824267514
25	0.0	9.294621852957223E-5	0.0	0.0026024941188280224	0.001626558824267514
26	0.0	9.294621852957223E-5	0.0	0.003206644539270242	0.001626558824267514
27	0.0	9.294621852957223E-5	0.0	0.0038572680689772476	0.001626558824267514
28	0.0	9.294621852957223E-5	0.0	0.004740257145008184	0.001626558824267514
29	0.0	9.294621852957223E-5	0.0	0.005762665548833479	0.001626558824267514
30	0.0	9.294621852957223E-5	0.0	0.007528643700895351	0.0016730319335323002
31	0.0	9.294621852957223E-5	0.0	0.012036535299579603	0.0017195050427970863
32	0.0	9.294621852957223E-5	0.0	0.015754384040762494	0.0018124512613266585
33	0.0	9.294621852957223E-5	0.0	0.020680533622829823	0.0019053974798562308
34	0.0	9.294621852957223E-5	0.0	0.025792575641956295	0.0021842361354449473
35	0.0	1.3941932779435836E-4	0.0	0.030811671442553194	0.0021842361354449473
36	0.0	1.3941932779435836E-4	0.0	0.03806147648785983	0.0021842361354449473
37	0.0	1.3941932779435836E-4	0.0	0.0508880546449408	0.0021842361354449473
38	0.0	1.3941932779435836E-4	0.0	0.06692127734129201	0.00227718235397452
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2300	0.0	28.034376	1
GGTATCA	1035	0.0	21.900196	1
TCAACGC	3325	0.0	18.988913	4
ATCAACG	3340	0.0	18.903633	3
CGGATCG	210	0.0	18.856585	26
CAACGCA	3440	0.0	18.609915	5
TATCAAC	3520	0.0	18.251156	2
AACGCAG	3555	0.0	17.88414	6
ATACCGT	555	0.0	17.04454	6
CAATACG	350	0.0	16.344273	38
GTCCTAT	375	0.0	15.844686	1
ACCGTCG	445	0.0	15.8197565	8
CGCAATA	340	0.0	15.529675	36
AACGCCG	340	0.0	15.528953	5
ATACGAA	355	0.0	15.4943	40
GGCGTTA	485	0.0	15.424016	42
GATATAC	315	0.0	15.369624	1
CGTCGTA	460	0.0	15.303895	10
GTATAGC	350	0.0	15.090177	1
GATCTAA	175	6.39302E-8	15.090177	1
>>END_MODULE