FastQCFastQC Report
Thu 2 Feb 2017
SRR4062018_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062018_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151040
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16491.0917637711864407No Hit
TATCAACGCAGAGTACTTTTTTTTT11140.737552966101695No Hit
GGTATCAACGCAGAGTACTTTTTTT7190.4760328389830508No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6010.3979078389830509No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3610.2390095338983051No Hit
GTACTTTTTTTTTTTTTTTTTTTTT3550.23503707627118642No Hit
GTATCAACGCAGAGTACATGGGGTG2460.1628707627118644No Hit
GTACATGGGGTGGTATCAACGCAAA2310.1529396186440678No Hit
TATCAACGCAGAGTACATGGGGTGG2130.14102224576271188No Hit
GGTATCAACGCAGAGTACATGGGGG1760.11652542372881357No Hit
ATCAACGCAGAGTACTTTTTTTTTT1730.11453919491525423No Hit
ACGCAGAGTACATGGGGTGGTATCA1630.10791843220338981No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1580.10460805084745763No Hit
GGTATCAACGCAGAGTACATGGGGT1550.10262182203389832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCAA250.00599160319.0086389
TTTAGGG250.006003118619.0023253
CAGCCGA250.006003118619.00232510
ACCGACA250.006003118619.0023255
CCCCCGG250.006003118619.00232513
GTCCACC307.723836E-418.9834121
ATTTTTG456.70606E-414.77958715
CGGCCGT400.00524559814.25174410
GCCCTCC400.00524559814.25174413
TTATGAG400.005257177614.2470122
GGGCAAG400.00526877914.24228319
ATACCCA500.001487234513.3016286
ACGCAAA604.0726128E-412.65980719
GTTATGA550.003066393812.0803531
CTGTGGT600.005814275211.0883729
TAACGGC600.005828685611.0846898
GTATCAA7050.010.6360951
GGGTGGT1105.9190697E-610.3649058
GGGGTGG1151.005099E-59.9142577
GGTATCA3150.09.6423671
Produced by FastQC (version 0.11.5)