##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062018_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151040
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.025754766949152	32.0	32.0	32.0	32.0	32.0
2	30.77723781779661	32.0	32.0	32.0	32.0	32.0
3	30.833222987288135	32.0	32.0	32.0	32.0	32.0
4	30.79077065677966	32.0	32.0	32.0	32.0	32.0
5	30.847927701271185	32.0	32.0	32.0	32.0	32.0
6	34.42517876059322	36.0	36.0	36.0	32.0	36.0
7	34.34339247881356	36.0	36.0	36.0	32.0	36.0
8	34.2446438029661	36.0	36.0	36.0	32.0	36.0
9	34.228330243644066	36.0	36.0	36.0	32.0	36.0
10	34.1249470338983	36.0	36.0	36.0	32.0	36.0
11	34.33646054025424	36.0	36.0	36.0	32.0	36.0
12	34.26300979872882	36.0	36.0	36.0	32.0	36.0
13	34.28270656779661	36.0	36.0	36.0	32.0	36.0
14	34.228979078389834	36.0	36.0	36.0	32.0	36.0
15	34.222782044491524	36.0	36.0	36.0	32.0	36.0
16	34.203701006355935	36.0	36.0	36.0	32.0	36.0
17	34.1373874470339	36.0	36.0	36.0	32.0	36.0
18	34.1790188029661	36.0	36.0	36.0	32.0	36.0
19	34.10922934322034	36.0	36.0	36.0	32.0	36.0
20	34.02670815677966	36.0	36.0	36.0	32.0	36.0
21	34.011261917372885	36.0	36.0	36.0	32.0	36.0
22	34.02971398305085	36.0	36.0	36.0	32.0	36.0
23	34.05295286016949	36.0	36.0	36.0	32.0	36.0
24	33.9921875	36.0	36.0	36.0	32.0	36.0
25	33.69900026483051	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	12.0
6	30.0
7	5.0
8	17.0
9	28.0
10	12.0
11	4.0
12	8.0
13	5.0
14	51.0
15	89.0
16	124.0
17	185.0
18	234.0
19	284.0
20	409.0
21	531.0
22	677.0
23	844.0
24	1060.0
25	1413.0
26	1747.0
27	1992.0
28	2663.0
29	3424.0
30	4512.0
31	6112.0
32	8716.0
33	12308.0
34	29605.0
35	73934.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.527572348863536	19.259264163560406	12.97611875078622	23.237044736789837
2	16.164592161016948	20.021848516949152	39.43061440677966	24.38294491525424
3	19.51045108152307	24.981295973834225	28.720114938723622	26.78813800591908
4	12.848203939745076	15.172984605197815	35.36964078794902	36.609170667108096
5	14.555268560831843	37.06934234055235	31.478243592290877	16.89714550632492
6	32.17735204101912	35.9977211600885	16.368562608476754	15.456364190415622
7	28.47192306927609	32.15741372797222	19.34137014626646	20.02929305648523
8	27.795268805800983	33.107091488755295	18.36204439554321	20.73559530990051
9	26.778955462312993	14.468948237118937	20.036987690655636	38.715108609912434
10	18.286627286408507	26.535929513315697	27.97655747595086	27.200885724324937
11	36.304267112207285	23.599066486328798	19.58920094412178	20.50746545734214
12	23.99443100076242	25.48877912951238	27.882785825570988	22.634004044154207
13	29.042138481529605	21.713649261449522	25.580100241321702	23.66411201569917
14	22.98314927015525	18.975300621793256	26.373182017049597	31.6683680910019
15	26.76956533269685	26.111199512026946	22.963242899196434	24.155992256079774
16	24.3314550878356	27.997348359297312	22.886973815048062	24.784222737819025
17	21.754118465975008	27.21734230501508	25.465875567635653	25.56266366137426
18	22.265234020270046	26.786554688691066	27.383785288639363	23.56442600239953
19	24.912848110494014	24.065188287846453	28.031096324377348	22.990867277282188
20	26.154814765723373	23.240771422890845	26.862615150109352	23.741798661276427
21	27.17603790464199	22.943573771578148	26.57963619495709	23.30075212882277
22	24.988898977380426	24.50045398211907	27.34032752987335	23.17031951062716
23	23.822839497992234	25.554951826868283	27.393747432312445	23.228461242827038
24	23.86538359577965	24.318037219659615	27.01010020677589	24.806478977784845
25	24.256300488399678	24.740724050867787	26.950782300978787	24.052193159753745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	196.0
1	196.0
2	173.0
3	150.0
4	150.0
5	150.0
6	331.0
7	512.0
8	512.0
9	512.0
10	549.0
11	586.0
12	586.0
13	586.0
14	489.0
15	392.0
16	392.0
17	392.0
18	615.5
19	839.0
20	839.0
21	839.0
22	1671.5
23	2504.0
24	2504.0
25	2504.0
26	3486.0
27	4468.0
28	4468.0
29	4468.0
30	5712.0
31	6956.0
32	6956.0
33	6956.0
34	8294.5
35	9633.0
36	9633.0
37	9633.0
38	11188.0
39	12743.0
40	12743.0
41	12743.0
42	14115.5
43	15488.0
44	15488.0
45	15488.0
46	17884.0
47	20280.0
48	20280.0
49	20280.0
50	21265.0
51	22250.0
52	22250.0
53	22250.0
54	20504.0
55	18758.0
56	18758.0
57	18758.0
58	16279.5
59	13801.0
60	13801.0
61	13801.0
62	11425.5
63	9050.0
64	9050.0
65	9050.0
66	7651.0
67	6252.0
68	6252.0
69	6252.0
70	4723.0
71	3194.0
72	3194.0
73	3194.0
74	2562.0
75	1930.0
76	1930.0
77	1930.0
78	1274.5
79	619.0
80	619.0
81	619.0
82	437.0
83	255.0
84	255.0
85	255.0
86	183.5
87	112.0
88	112.0
89	112.0
90	68.0
91	24.0
92	24.0
93	24.0
94	17.5
95	11.0
96	11.0
97	11.0
98	24.0
99	37.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.620762711864406E-4
2	0.0
3	0.001986228813559322
4	0.009931144067796611
5	0.03310381355932203
6	0.0569385593220339
7	0.09997351694915255
8	0.11189088983050846
9	0.11851165254237288
10	0.13440148305084745
11	0.1403601694915254
12	0.13572563559322035
13	0.1350635593220339
14	0.12314618644067797
15	0.1403601694915254
16	0.1257944915254237
17	0.12910487288135594
18	0.1171875
19	0.10195974576271186
20	0.0993114406779661
21	0.0893802966101695
22	0.10129766949152544
23	0.08342161016949153
24	0.10063559322033898
25	0.09202860169491525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	151040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.267876059322035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.96385426192425	25.983845338983052
2	15.75941345397956	13.007150423728813
3	7.224334600760455	8.943988347457626
4	4.166466124400379	6.877648305084747
5	2.5765670372687746	5.3164724576271185
6	1.6717203317771254	4.139300847457627
7	1.2112752883797788	3.4990730932203387
8	0.832651489627954	2.7489406779661016
9	0.6529656190338675	2.425185381355932
>10	2.8108004042932087	19.5067531779661
>50	0.09465594968795624	2.7019332627118646
>100	0.02887808634547817	2.1464512711864407
>500	0.003208676260608686	0.8739406779661016
>1k	0.003208676260608686	1.8293167372881356
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1649	1.0917637711864407	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1114	0.737552966101695	No Hit
GGTATCAACGCAGAGTACTTTTTTT	719	0.4760328389830508	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	601	0.3979078389830509	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	361	0.2390095338983051	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	355	0.23503707627118642	No Hit
GTATCAACGCAGAGTACATGGGGTG	246	0.1628707627118644	No Hit
GTACATGGGGTGGTATCAACGCAAA	231	0.1529396186440678	No Hit
TATCAACGCAGAGTACATGGGGTGG	213	0.14102224576271188	No Hit
GGTATCAACGCAGAGTACATGGGGG	176	0.11652542372881357	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	173	0.11453919491525423	No Hit
ACGCAGAGTACATGGGGTGGTATCA	163	0.10791843220338981	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	158	0.10460805084745763	No Hit
GGTATCAACGCAGAGTACATGGGGT	155	0.10262182203389832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTCAA	25	0.005991603	19.008638	9
TTTAGGG	25	0.0060031186	19.002325	3
CAGCCGA	25	0.0060031186	19.002325	10
ACCGACA	25	0.0060031186	19.002325	5
CCCCCGG	25	0.0060031186	19.002325	13
GTCCACC	30	7.723836E-4	18.983412	1
ATTTTTG	45	6.70606E-4	14.779587	15
CGGCCGT	40	0.005245598	14.251744	10
GCCCTCC	40	0.005245598	14.251744	13
TTATGAG	40	0.0052571776	14.247012	2
GGGCAAG	40	0.005268779	14.242283	19
ATACCCA	50	0.0014872345	13.301628	6
ACGCAAA	60	4.0726128E-4	12.659807	19
GTTATGA	55	0.0030663938	12.080353	1
CTGTGGT	60	0.0058142752	11.088372	9
TAACGGC	60	0.0058286856	11.084689	8
GTATCAA	705	0.0	10.636095	1
GGGTGGT	110	5.9190697E-6	10.364905	8
GGGGTGG	115	1.005099E-5	9.914257	7
GGTATCA	315	0.0	9.642367	1
>>END_MODULE