FastQCFastQC Report
Thu 2 Feb 2017
SRR4062018_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062018_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151040
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.3125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.19134004237288135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.13440148305084745No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1760.11652542372881357No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1730.11453919491525423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATCT254.4277782E-543.9943734
TACCCGG207.8396866E-443.994376
CGTGCAT250.002340149435.21882240
GCGCATC250.00234777235.195518
GGCGCAT250.00234777235.195517
TGCCAGG401.8111825E-532.99577726
CCATATC401.8111825E-532.9957773
ACCTATA353.194606E-431.4453751
TCGTGCA300.005714352729.34901639
GTTTAAT300.005723617529.3392962
CGTTTTT300.005732894429.3295830
CGGGAAA508.263931E-526.41411840
TCCATAT508.282592E-526.4053692
AGAGTTA450.001388227824.45751640
CCCCCGA450.001388227824.45751644
TTTCACG450.001393600124.44131925
TTCTGGT450.001393600124.44131937
GTCCATA500.002562118522.0117651
CCGTGCG500.002572000721.99718713
TTCGGTT500.002572000721.99718714