##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151040
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07318591101695	32.0	32.0	32.0	32.0	32.0
2	31.40153601694915	32.0	32.0	32.0	32.0	32.0
3	31.539261122881356	32.0	32.0	32.0	32.0	32.0
4	31.674947033898306	32.0	32.0	32.0	32.0	32.0
5	31.62564221398305	32.0	32.0	32.0	32.0	32.0
6	35.26571769067797	36.0	36.0	36.0	36.0	36.0
7	35.27071636652543	36.0	36.0	36.0	36.0	36.0
8	35.205442266949156	36.0	36.0	36.0	36.0	36.0
9	35.26165916313559	36.0	36.0	36.0	36.0	36.0
10	35.18848649364407	36.0	36.0	36.0	36.0	36.0
11	35.28009798728814	36.0	36.0	36.0	36.0	36.0
12	35.20837526483051	36.0	36.0	36.0	36.0	36.0
13	35.2298000529661	36.0	36.0	36.0	36.0	36.0
14	35.18332891949152	36.0	36.0	36.0	36.0	36.0
15	35.1931342690678	36.0	36.0	36.0	36.0	36.0
16	35.18306408898305	36.0	36.0	36.0	36.0	36.0
17	35.17046477754237	36.0	36.0	36.0	36.0	36.0
18	35.15858712923729	36.0	36.0	36.0	36.0	36.0
19	35.11090439618644	36.0	36.0	36.0	36.0	36.0
20	35.07368246822034	36.0	36.0	36.0	36.0	36.0
21	35.05581302966102	36.0	36.0	36.0	36.0	36.0
22	35.071696239406776	36.0	36.0	36.0	36.0	36.0
23	35.05333686440678	36.0	36.0	36.0	36.0	36.0
24	35.014678230932205	36.0	36.0	36.0	36.0	36.0
25	35.00319120762712	36.0	36.0	36.0	36.0	36.0
26	34.96904131355932	36.0	36.0	36.0	36.0	36.0
27	34.98818193855932	36.0	36.0	36.0	36.0	36.0
28	34.96475105932203	36.0	36.0	36.0	36.0	36.0
29	34.94183659957627	36.0	36.0	36.0	36.0	36.0
30	34.897000794491525	36.0	36.0	36.0	32.0	36.0
31	34.87119968220339	36.0	36.0	36.0	32.0	36.0
32	34.86972987288136	36.0	36.0	36.0	32.0	36.0
33	34.77674788135593	36.0	36.0	36.0	32.0	36.0
34	34.74892743644068	36.0	36.0	36.0	32.0	36.0
35	34.73640095338983	36.0	36.0	36.0	32.0	36.0
36	34.72446371822034	36.0	36.0	36.0	32.0	36.0
37	34.71581038135593	36.0	36.0	36.0	32.0	36.0
38	34.678952595338984	36.0	36.0	36.0	32.0	36.0
39	34.608050847457626	36.0	36.0	36.0	32.0	36.0
40	34.589757680084745	36.0	36.0	36.0	32.0	36.0
41	34.50093352754237	36.0	36.0	36.0	32.0	36.0
42	34.52045153601695	36.0	36.0	36.0	32.0	36.0
43	34.41904131355932	36.0	36.0	36.0	32.0	36.0
44	34.28075344279661	36.0	36.0	36.0	32.0	36.0
45	34.21366525423729	36.0	36.0	36.0	32.0	36.0
46	34.12380826271186	36.0	36.0	36.0	32.0	36.0
47	34.06442002118644	36.0	36.0	36.0	32.0	36.0
48	34.01584348516949	36.0	36.0	36.0	32.0	36.0
49	33.87538400423729	36.0	36.0	36.0	32.0	36.0
50	33.50493908898305	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	19.0
23	38.0
24	105.0
25	210.0
26	488.0
27	946.0
28	1657.0
29	2429.0
30	3633.0
31	5160.0
32	7687.0
33	11919.0
34	23856.0
35	92889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15648655271297	20.10702199381445	13.247283058603813	23.489208394868772
2	15.792331274126163	20.179568162405896	39.353369220480836	24.67473134298711
3	18.979740466101696	25.17876059322034	28.769200211864405	27.07229872881356
4	12.854872881355933	15.681938559322035	35.32309322033898	36.14009533898305
5	14.227356991525426	37.253707627118644	31.404263771186443	17.11467161016949
6	32.50662076271186	36.037473516949156	15.989804025423728	15.466101694915254
7	28.497748940677965	32.10672669491525	19.40280720338983	19.992717161016948
8	27.676774364406782	33.373940677966104	18.307733050847457	20.64155190677966
9	26.86242055084746	14.05918961864407	20.0880561440678	38.99033368644068
10	18.626191737288135	26.192399364406782	28.013771186440678	27.167637711864405
11	36.73795021186441	23.12831038135593	19.671610169491522	20.462129237288135
12	23.96451271186441	25.432997881355934	27.766816737288135	22.835672669491526
13	29.354475635593218	21.70219809322034	25.364804025423727	23.578522245762713
14	23.146186440677965	18.856594279661017	26.293697033898304	31.70352224576271
15	26.857786016949152	25.825609110169488	22.976694915254235	24.339909957627118
16	24.636520127118644	27.650291313559322	23.207759533898304	24.505429025423727
17	22.151085805084747	26.675052966101692	25.744173728813557	25.429687499999996
18	22.289754896118858	26.464863147022605	27.424886452415947	23.82049550444259
19	24.986096398305087	23.838056144067796	28.392478813559325	22.783368644067796
20	26.54479968749793	22.892100715709187	26.826183965730703	23.736915631062175
21	27.047139830508478	22.944915254237287	26.72801906779661	23.279925847457626
22	24.900688559322035	24.43458686440678	27.317929025423727	23.346795550847457
23	24.061837923728813	25.160222457627118	27.569518008474574	23.20842161016949
24	23.91551906779661	23.957229872881356	27.44438559322034	24.682865466101696
25	24.529925847457626	24.034030720338983	27.143802966101692	24.292240466101696
26	23.826958600096663	24.547302352372565	27.38166963499494	24.244069412535836
27	23.74668961864407	25.088718220338986	27.929025423728817	23.235566737288135
28	22.74328314728744	25.445914273229253	27.589745626928323	24.22105695255499
29	23.09683655768747	24.682530224181995	27.538765078986742	24.68186813914379
30	23.482014579016017	24.950509471063764	27.499519991525368	24.06795595839485
31	24.165441809345992	25.280393013678676	26.55887922244733	23.995285954528
32	23.047689669555545	25.291481008216422	27.7358827852409	23.924946536987136
33	22.91048728813559	25.642213983050848	28.00979872881356	23.4375
34	23.540783898305083	24.306806144067796	28.008474576271187	24.143935381355934
35	24.28032309322034	24.61268538135593	26.46649894067797	24.64049258474576
36	24.220074152542374	25.836202330508474	26.414856991525426	23.528866525423727
37	23.734267308443517	25.91847138818451	26.671919173193682	23.675342130178297
38	22.897907838983052	25.740201271186443	27.468882415254235	23.89300847457627
39	23.88704978813559	24.360434322033896	27.824417372881356	23.928098516949152
40	24.493011738690008	25.014731294566307	26.91821317672919	23.5740437900145
41	24.749735169491522	26.009004237288135	26.096398305084744	23.14486228813559
42	22.73910020856093	26.058860529016453	27.58896944416857	23.613069818254047
43	23.45849030370042	26.40346405185484	26.4875494084231	23.650496236021638
44	23.62198989624784	25.081273628942018	27.306615110604966	23.990121364205173
45	24.631549676240418	25.73723169003827	26.15831777433494	23.47290085938638
46	25.130928183160417	26.279653329978746	24.85748525195813	23.731933234902705
47	23.469435046577374	26.561351703864567	25.983355292341713	23.985857957216346
48	23.262665854498266	27.112079239386638	25.946132047988556	23.67912285812654
49	23.813023471380806	25.594729698414277	25.545072334227164	25.047174495977753
50	24.675582627118644	26.424126059322035	25.141022245762713	23.75926906779661
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	81.5
2	15.0
3	34.0
4	53.0
5	58.0
6	63.0
7	56.5
8	50.0
9	74.5
10	99.0
11	174.5
12	250.0
13	481.5
14	713.0
15	1037.5
16	1362.0
17	1417.5
18	1473.0
19	1423.0
20	1373.0
21	1306.5
22	1240.0
23	1138.5
24	1037.0
25	952.5
26	868.0
27	1030.5
28	1193.0
29	1454.5
30	1716.0
31	2051.0
32	2386.0
33	2860.5
34	3335.0
35	3743.0
36	4151.0
37	4916.0
38	5681.0
39	6159.5
40	6638.0
41	7452.0
42	8266.0
43	8331.5
44	8397.0
45	8690.0
46	8983.0
47	9554.5
48	10126.0
49	11012.5
50	11899.0
51	12547.5
52	13196.0
53	12499.0
54	11802.0
55	11088.5
56	10375.0
57	9629.0
58	8883.0
59	7853.0
60	6823.0
61	6132.0
62	5441.0
63	4544.5
64	3648.0
65	3212.5
66	2777.0
67	2392.0
68	2007.0
69	1839.5
70	1672.0
71	1458.0
72	1244.0
73	1011.0
74	778.0
75	591.5
76	405.0
77	318.5
78	232.0
79	190.0
80	148.0
81	103.0
82	58.0
83	40.0
84	22.0
85	13.5
86	5.0
87	2.5
88	0.0
89	1.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02846927966101695
2	0.007282838983050848
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0013241525423728813
19	0.0
20	6.620762711864406E-4
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	6.620762711864406E-4
27	0.0
28	0.0013241525423728813
29	0.0013241525423728813
30	6.620762711864406E-4
31	0.0013241525423728813
32	6.620762711864406E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	6.620762711864406E-4
38	0.0
39	0.0
40	6.620762711864406E-4
41	0.0
42	0.0033103813559322032
43	0.001986228813559322
44	0.0046345338983050845
45	0.0013241525423728813
46	0.001986228813559322
47	6.620762711864406E-4
48	0.0026483050847457626
49	0.0033103813559322032
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	151040.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.13572563559322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.38472709137048	29.060513771186443
2	15.267041203995715	13.78177966101695
3	7.197864257110586	9.746424788135593
4	3.9047716838044386	7.04978813559322
5	2.4789872823551846	5.594544491525424
6	1.6780836988250483	4.544491525423728
7	1.1514822583720827	3.6381091101694913
8	0.8331744238921567	3.0084745762711864
9	0.5896762647969137	2.3953919491525424
>10	2.432047878192246	17.78667902542373
>50	0.06600853710413213	2.086864406779661
>100	0.016135420181010076	1.306938559322034
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.3125	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	289	0.19134004237288135	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	203	0.13440148305084745	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	176	0.11652542372881357	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	173	0.11453919491525423	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	6.620762711864406E-4	0.0
23	0.0	0.0	0.0	0.0013241525423728813	0.0
24	0.0	0.0	0.0	0.0013241525423728813	0.0
25	0.0	0.0	0.0	0.001986228813559322	0.0
26	0.0	0.0	0.0	0.001986228813559322	0.0
27	0.0	0.0	0.0	0.0026483050847457626	0.0
28	0.0	0.0	0.0	0.0026483050847457626	0.0
29	0.0	0.0	0.0	0.007282838983050847	0.0
30	0.0	0.0	0.0	0.015889830508474576	0.0
31	0.0	0.0	0.0	0.033103813559322036	0.0
32	0.0	0.0	0.0	0.050979872881355935	0.0
33	0.0	0.0	0.0	0.06885593220338983	0.0
34	0.0	0.0	0.0	0.08209745762711865	0.0
35	0.0	0.0	0.0	0.1026218220338983	0.0
36	0.0	0.0	0.0	0.1330773305084746	0.0
37	0.0	0.0	0.0	0.1840572033898305	0.0
38	0.0	0.0	0.0	0.23834745762711865	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATATCT	25	4.4277782E-5	43.994373	4
TACCCGG	20	7.8396866E-4	43.99437	6
CGTGCAT	25	0.0023401494	35.218822	40
GCGCATC	25	0.002347772	35.1955	18
GGCGCAT	25	0.002347772	35.1955	17
TGCCAGG	40	1.8111825E-5	32.995777	26
CCATATC	40	1.8111825E-5	32.995777	3
ACCTATA	35	3.194606E-4	31.445375	1
TCGTGCA	30	0.0057143527	29.349016	39
GTTTAAT	30	0.0057236175	29.339296	2
CGTTTTT	30	0.0057328944	29.32958	30
CGGGAAA	50	8.263931E-5	26.414118	40
TCCATAT	50	8.282592E-5	26.405369	2
AGAGTTA	45	0.0013882278	24.457516	40
CCCCCGA	45	0.0013882278	24.457516	44
TTTCACG	45	0.0013936001	24.441319	25
TTCTGGT	45	0.0013936001	24.441319	37
GTCCATA	50	0.0025621185	22.011765	1
CCGTGCG	50	0.0025720007	21.997187	13
TTCGGTT	50	0.0025720007	21.997187	14
>>END_MODULE