FastQCFastQC Report
Thu 2 Feb 2017
SRR4062016_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062016_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506465
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23850.15831765092451533No Hit
TATCAACGCAGAGTACTTTTTTTTT17130.11370991028666448No Hit
GCGCAAGACGGACCAGAGCGAAAGC16250.10786842044123161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT350.00216878616.2872526
TTAACCG400.00527801814.25045
TAGGACG400.00528034714.2494524
TACACGG852.6978887E-713.412145
TCGCGTA1650.013.2445569
CGCATCG2450.013.18535713
AACCGTG655.445729E-513.1555267
GGTATCA7800.013.0254861
CGATCAA751.4750527E-512.66954910
ACAATAC1457.2759576E-1212.4461413
TTATCCT1001.4430043E-712.3495254
CGCGTAA1700.012.29691410
CCGATCA951.037668E-612.0019319
CGCCAGT2850.011.9987418
GCGTAAC1750.011.94517711
CGTCGTA2150.011.93294810
ATCGCCA2800.011.87493816
GCATCGC2650.011.83091414
GTTTTCG1459.640644E-1111.79382615
GTACCGA1052.716115E-711.7630176
Produced by FastQC (version 0.11.5)