##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062016_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506465
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278551443279465	32.0	32.0	32.0	32.0	32.0
2	30.836256401575874	32.0	32.0	32.0	32.0	32.0
3	30.866844566584685	32.0	32.0	32.0	32.0	32.0
4	30.865084153963085	32.0	32.0	32.0	32.0	32.0
5	30.788955601358147	32.0	32.0	32.0	32.0	32.0
6	34.43480333097682	36.0	36.0	36.0	32.0	36.0
7	34.37693540838984	36.0	36.0	36.0	32.0	36.0
8	34.337740339138314	36.0	36.0	36.0	32.0	36.0
9	34.46280796434036	36.0	36.0	36.0	32.0	36.0
10	34.169367360011684	36.0	36.0	36.0	32.0	36.0
11	34.44114864932143	36.0	36.0	36.0	32.0	36.0
12	34.27860388392694	36.0	36.0	36.0	32.0	36.0
13	34.36555512408187	36.0	36.0	36.0	32.0	36.0
14	34.27069862227135	36.0	36.0	36.0	32.0	36.0
15	34.217948641355754	36.0	36.0	36.0	32.0	36.0
16	34.21884544280817	36.0	36.0	36.0	32.0	36.0
17	34.15542345822837	36.0	36.0	36.0	32.0	36.0
18	34.162759174624036	36.0	36.0	36.0	32.0	36.0
19	34.1640058016615	36.0	36.0	36.0	32.0	36.0
20	34.157404918136166	36.0	36.0	36.0	32.0	36.0
21	34.139800128114494	36.0	36.0	36.0	32.0	36.0
22	34.10487332928412	36.0	36.0	36.0	32.0	36.0
23	34.08303412293017	36.0	36.0	36.0	32.0	36.0
24	34.04980069234931	36.0	36.0	36.0	32.0	36.0
25	33.70268077917509	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	28.0
5	148.0
6	310.0
7	85.0
8	265.0
9	187.0
10	122.0
11	32.0
12	77.0
13	64.0
14	178.0
15	302.0
16	487.0
17	651.0
18	919.0
19	1189.0
20	1907.0
21	2629.0
22	3977.0
23	5682.0
24	8227.0
25	11614.0
26	16307.0
27	21508.0
28	28766.0
29	38702.0
30	51222.0
31	70249.0
32	100157.0
33	143558.0
34	315815.0
35	681100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.565275229479596	17.14419045545427	11.475838592932268	25.814695722133862
2	17.23406599825817	18.95624455678994	36.9210946959237	26.88859474902819
3	19.55931094042387	22.338875468897594	27.80088937037118	30.30092422030736
4	13.156151164056713	15.106248224106478	34.51633290933184	37.221267702504974
5	15.366236698374752	35.9452163952388	32.85130401580424	15.837242890582202
6	35.54902236900489	34.1270136488393	16.196538503436784	14.127425478719022
7	31.47603361692994	29.67278183617229	19.498390855620908	19.35279369127686
8	28.80224833046577	32.50429802473616	18.548194696699344	20.145258948098736
9	26.989860616710576	14.433002708975243	18.351645722397826	40.225490951916356
10	17.055225082042295	25.986717690093176	30.10727815012826	26.850779077736277
11	37.55734314684167	21.0889830029628	21.407532036048156	19.94614181414738
12	24.637528948494943	23.275520541134117	27.969696808553067	24.117253701817873
13	29.624144809300702	18.97847043082402	25.038335275299456	26.359049484575824
14	23.746618026954767	19.179301228376474	24.35811464182488	32.71596610284388
15	25.594731636239874	26.765250176817496	21.584231601674052	26.055786585268574
16	26.37645577881799	25.479344440109607	22.796916404084257	25.347283376988145
17	24.407902345261025	25.788034556144428	24.480747271970788	25.323315826623755
18	25.250462379304544	24.70723730774624	25.35280720560743	24.68949310734178
19	25.95077511910853	24.770853129381436	24.71104968337398	24.56732206813606
20	26.076588404764816	24.01417378500922	24.386856890750497	25.522380919475466
21	26.90706429369687	23.74327436344815	24.217208327630356	25.13245301522462
22	26.449338200066315	23.652295717155365	24.56942636474848	25.328939718029837
23	24.88508011339515	23.937684488024345	25.044994648477026	26.132240750103474
24	24.933257321761502	24.599776483454857	24.77844354346812	25.68852265131552
25	25.16655559638099	24.107257352398886	24.77407772350874	25.95210932771138
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	232.0
1	232.0
2	331.5
3	431.0
4	431.0
5	431.0
6	951.5
7	1472.0
8	1472.0
9	1472.0
10	1660.5
11	1849.0
12	1849.0
13	1849.0
14	2510.5
15	3172.0
16	3172.0
17	3172.0
18	5378.0
19	7584.0
20	7584.0
21	7584.0
22	12028.5
23	16473.0
24	16473.0
25	16473.0
26	24880.5
27	33288.0
28	33288.0
29	33288.0
30	41853.5
31	50419.0
32	50419.0
33	50419.0
34	65174.0
35	79929.0
36	79929.0
37	79929.0
38	96580.0
39	113231.0
40	113231.0
41	113231.0
42	134155.0
43	155079.0
44	155079.0
45	155079.0
46	175340.5
47	195602.0
48	195602.0
49	195602.0
50	205242.0
51	214882.0
52	214882.0
53	214882.0
54	202974.5
55	191067.0
56	191067.0
57	191067.0
58	176269.5
59	161472.0
60	161472.0
61	161472.0
62	142032.0
63	122592.0
64	122592.0
65	122592.0
66	100468.0
67	78344.0
68	78344.0
69	78344.0
70	59082.0
71	39820.0
72	39820.0
73	39820.0
74	29837.0
75	19854.0
76	19854.0
77	19854.0
78	15817.0
79	11780.0
80	11780.0
81	11780.0
82	8147.0
83	4514.0
84	4514.0
85	4514.0
86	3236.5
87	1959.0
88	1959.0
89	1959.0
90	1327.0
91	695.0
92	695.0
93	695.0
94	427.5
95	160.0
96	160.0
97	160.0
98	362.5
99	565.0
100	565.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.629473635298529E-4
2	6.63805664253733E-5
3	3.9828339855223986E-4
4	0.012014882522992569
5	0.03584550586970159
6	0.0656503801946942
7	0.10806756214050775
8	0.11311248518883611
9	0.12194110052341077
10	0.13661120570341828
11	0.14231993441600035
12	0.1373413919340974
13	0.13116799925653766
14	0.11921949729997046
15	0.13866900326260484
16	0.1233350924183436
17	0.1260566956417839
18	0.11603323011155253
19	0.10189416946294802
20	0.09512335168755995
21	0.09313193469479875
22	0.10315540022503011
23	0.08536540842303007
24	0.09499059055470921
25	0.0937293597926271
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1506465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.69785788616136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06632891749457	37.803336044636524
2	13.301847045994522	13.221466082305822
3	4.163562201906891	6.207603678316854
4	1.860861836222284	3.699233883294254
5	1.045891622950722	2.598928662086582
6	0.6676696367675717	1.9909050433787854
7	0.4742790717785759	1.6499457735342533
8	0.3548527395935778	1.4108336818269325
9	0.2748711787653781	1.2294457901355074
>10	1.5964242915000826	14.453000051501586
>50	0.10791158311085787	3.6852555660004374
>100	0.07491297649929925	7.600914164626022
>500	0.00790667022173835	2.677883964830281
>1k	0.0026802271938096097	1.771247613526197
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2385	0.15831765092451533	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1713	0.11370991028666448	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1625	0.10786842044123161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.63805664253733E-5
2	0.0	0.0	0.0	0.0	6.63805664253733E-5
3	0.0	0.0	0.0	0.0	6.63805664253733E-5
4	0.0	0.0	0.0	0.0	6.63805664253733E-5
5	0.0	0.0	0.0	0.0	6.63805664253733E-5
6	0.0	0.0	0.0	0.0	1.9914169927611993E-4
7	0.0	0.0	0.0	0.0	1.9914169927611993E-4
8	0.0	0.0	0.0	0.0	1.9914169927611993E-4
9	0.0	0.0	0.0	0.0	1.9914169927611993E-4
10	0.0	0.0	0.0	0.0	1.9914169927611993E-4
11	0.0	0.0	0.0	0.0	2.655222657014932E-4
12	0.0	6.63805664253733E-5	0.0	0.0	5.974250978283597E-4
13	0.0	6.63805664253733E-5	0.0	0.0	5.974250978283597E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACCGT	35	0.002168786	16.287252	6
TTAACCG	40	0.005278018	14.2504	5
TAGGACG	40	0.005280347	14.249452	4
TACACGG	85	2.6978887E-7	13.41214	5
TCGCGTA	165	0.0	13.244556	9
CGCATCG	245	0.0	13.185357	13
AACCGTG	65	5.445729E-5	13.155526	7
GGTATCA	780	0.0	13.025486	1
CGATCAA	75	1.4750527E-5	12.669549	10
ACAATAC	145	7.2759576E-12	12.446141	3
TTATCCT	100	1.4430043E-7	12.349525	4
CGCGTAA	170	0.0	12.296914	10
CCGATCA	95	1.037668E-6	12.001931	9
CGCCAGT	285	0.0	11.99874	18
GCGTAAC	175	0.0	11.945177	11
CGTCGTA	215	0.0	11.932948	10
ATCGCCA	280	0.0	11.874938	16
GCATCGC	265	0.0	11.830914	14
GTTTTCG	145	9.640644E-11	11.793826	15
GTACCGA	105	2.716115E-7	11.763017	6
>>END_MODULE