FastQCFastQC Report
Thu 2 Feb 2017
SRR4062016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506465
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT17000.11284696292313463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17150.025.1554971
GGTATCA9000.023.723031
CTATACA1800.020.7764914
ACTATAC1101.2669807E-719.9987623
GTATTAT1553.274181E-1119.8809551
GTGTACG1102.7839615E-618.0090481
TATACAG2400.017.4155885
TATACTG1651.6607373E-917.3322625
GTATAGA2301.8189894E-1216.2690431
GTATAAC1105.598602E-516.0080431
CGTCGTA3300.015.99901110
ATACCGT3450.015.9410436
CAACGCA27400.015.8968285
TCAACGC27300.015.87447554
TATCAAC27350.015.8465072
ATAATAC1259.448815E-615.839023
TCTACAC4750.015.7463943
ATCAACG27550.015.7304253
AACGCAG27750.015.6963276
ATACGAA4250.015.53102840
Produced by FastQC (version 0.11.5)