##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1506465
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.306708088140116	32.0	32.0	32.0	32.0	32.0
2	31.446755815767375	32.0	32.0	32.0	32.0	32.0
3	31.575570623944134	32.0	32.0	32.0	32.0	32.0
4	31.64947011712851	32.0	32.0	32.0	32.0	32.0
5	31.620660287494232	32.0	32.0	32.0	32.0	32.0
6	35.27349457172918	36.0	36.0	36.0	36.0	36.0
7	35.27466818014358	36.0	36.0	36.0	36.0	36.0
8	35.226698927621946	36.0	36.0	36.0	36.0	36.0
9	35.318091691476404	36.0	36.0	36.0	36.0	36.0
10	35.19582067953786	36.0	36.0	36.0	36.0	36.0
11	35.31958990086063	36.0	36.0	36.0	36.0	36.0
12	35.23542531688423	36.0	36.0	36.0	36.0	36.0
13	35.27601305041936	36.0	36.0	36.0	36.0	36.0
14	35.24265349676229	36.0	36.0	36.0	36.0	36.0
15	35.21311613611999	36.0	36.0	36.0	36.0	36.0
16	35.226135356613	36.0	36.0	36.0	36.0	36.0
17	35.20380825309582	36.0	36.0	36.0	36.0	36.0
18	35.202034564360936	36.0	36.0	36.0	36.0	36.0
19	35.19368720813295	36.0	36.0	36.0	36.0	36.0
20	35.1934077459483	36.0	36.0	36.0	36.0	36.0
21	35.18735516590163	36.0	36.0	36.0	36.0	36.0
22	35.17920562376159	36.0	36.0	36.0	36.0	36.0
23	35.14490877650659	36.0	36.0	36.0	36.0	36.0
24	35.12522892997846	36.0	36.0	36.0	36.0	36.0
25	35.111976713697295	36.0	36.0	36.0	36.0	36.0
26	35.070408539196066	36.0	36.0	36.0	36.0	36.0
27	35.05768803125197	36.0	36.0	36.0	36.0	36.0
28	35.0413849641379	36.0	36.0	36.0	36.0	36.0
29	35.01938578061887	36.0	36.0	36.0	36.0	36.0
30	35.00232597504755	36.0	36.0	36.0	36.0	36.0
31	35.0037511658087	36.0	36.0	36.0	36.0	36.0
32	34.98309353353712	36.0	36.0	36.0	36.0	36.0
33	34.953924585038486	36.0	36.0	36.0	36.0	36.0
34	34.95228963168743	36.0	36.0	36.0	36.0	36.0
35	34.935145522796745	36.0	36.0	36.0	36.0	36.0
36	34.917856040465594	36.0	36.0	36.0	32.0	36.0
37	34.91018709362647	36.0	36.0	36.0	32.0	36.0
38	34.892722366599955	36.0	36.0	36.0	32.0	36.0
39	34.8819156103859	36.0	36.0	36.0	32.0	36.0
40	34.86628696982671	36.0	36.0	36.0	32.0	36.0
41	34.8386885855297	36.0	36.0	36.0	32.0	36.0
42	34.805506931790646	36.0	36.0	36.0	32.0	36.0
43	34.81392996186437	36.0	36.0	36.0	32.0	36.0
44	34.758887196184446	36.0	36.0	36.0	32.0	36.0
45	34.74954877809972	36.0	36.0	36.0	32.0	36.0
46	34.73757438772225	36.0	36.0	36.0	32.0	36.0
47	34.71029662156107	36.0	36.0	36.0	32.0	36.0
48	34.69332775736575	36.0	36.0	36.0	32.0	36.0
49	34.684224990291845	36.0	36.0	36.0	32.0	36.0
50	34.257733833842806	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	4.0
21	27.0
22	80.0
23	248.0
24	628.0
25	1533.0
26	3081.0
27	6221.0
28	10611.0
29	17628.0
30	26708.0
31	39948.0
32	61727.0
33	107280.0
34	249396.0
35	981338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33475719439389	17.604128555198244	11.826501876827274	26.234612373580596
2	16.721676695043524	19.426815862006702	36.622008082889444	27.229499360060327
3	18.91476923097348	22.745132140649986	28.139024987669792	30.201073640706745
4	12.895287975492295	15.533716349201606	34.75208517954284	36.81891049576326
5	15.027299007942435	36.26735436933483	32.71035171743121	15.994994905291527
6	35.71768535660731	34.3368995280993	15.9648341377573	13.980580977536091
7	31.58181570763343	29.554486828436104	19.5466207313147	19.31707673261576
8	28.872559269548248	32.61230762082093	18.510420089414623	20.0047130202162
9	27.117058809862826	14.19163405721341	18.336370244247295	40.35493688867648
10	17.056221020733968	25.997351415399628	30.136378873720933	26.81004869014547
11	37.82656749410043	20.94061262624754	21.430965870431773	19.80185400922026
12	24.70918654544682	23.194845678356735	27.933823841791106	24.162143934405336
13	29.669989014016256	18.916071730840077	25.020428619317407	26.393510635826257
14	23.911142973782994	19.052218272578518	24.37281981327147	32.663818940367015
15	25.645999077310126	26.698662099683695	21.56558565914243	26.089753163863747
16	26.430174050422846	25.395430346640467	22.813682407763896	25.360713195172792
17	24.589950646048862	25.730966202334603	24.531137464195982	25.147945687420552
18	25.424304862246956	24.57357095821739	25.329845253520826	24.672278926014826
19	26.13980411094848	24.60893548804652	24.66980646745859	24.581453933546417
20	26.224009484467924	23.934407947623008	24.389314573275282	25.452267994633786
21	27.088448785733487	23.676686813168576	24.177793709113722	25.05707069198421
22	26.52268721809003	23.62955661100656	24.566651067233558	25.281105103669848
23	24.9832555021192	23.73171630273521	25.048507598915343	26.236520596230246
24	25.094228152278536	24.389923329682368	24.886521291778685	25.62932722626041
25	25.360414003141134	23.937543728285213	24.8128396202493	25.88920264832435
26	25.217977763778816	24.831774602860612	25.035297959920634	24.91494967343994
27	25.662580702548627	24.236918652893078	24.677023853303577	25.423476791254718
28	25.375770775677974	23.949690561238206	25.06118742436624	25.613351238717584
29	25.097762238783478	24.465751187717437	25.037422143686427	25.39906442981265
30	25.191509586764134	24.582064347221152	25.123867205858534	25.102558860156183
31	25.6730007129287	24.308545452855764	24.39855768018045	25.619896154035082
32	25.680220968509598	24.179284003082728	24.147885870109974	25.992609158297704
33	25.107271066550545	24.178539945196167	24.684692100176306	26.02949688807698
34	25.846258830339323	24.072689906230128	24.828039887046003	25.253011376384542
35	26.209587484334172	24.014048792403933	24.904876585168978	24.871487138092913
36	25.12534327095986	24.615539843992185	24.58069000035182	25.678426884696137
37	26.201508191497837	24.25028544117257	24.513090464937203	25.035115902392395
38	25.40377639042394	24.01409923230875	24.906386806198615	25.675737571068698
39	25.926144736667418	24.151719926184565	24.229119923529332	25.69301541361868
40	25.99302206096138	24.394442076866195	24.652928517280888	24.95960734489154
41	24.93902868074926	24.75588238808427	25.013574997046028	25.29151393412044
42	26.19834902763902	24.63301214496958	24.495862611483783	24.672776215907618
43	25.749169212467855	23.785815472895017	24.750299056958237	25.714716257678894
44	25.223148198550447	24.529971494917014	24.585136199065584	25.661744107466948
45	25.572129659931775	24.638666306433805	24.378382576589868	25.410821457044552
46	25.35660516134857	24.56293961609848	24.57156930168584	25.508885920867108
47	25.601780614771457	24.269439462151315	24.93398369407201	25.194796229005224
48	26.26855585310124	24.868261906879855	23.836794791283577	25.026387448735328
49	25.45276277898963	24.906234524547635	24.074991137917895	25.566011558544837
50	25.54278738506821	24.952055744780566	24.152752488155826	25.3524043819954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	73.5
2	56.0
3	130.0
4	204.0
5	227.5
6	251.0
7	186.5
8	122.0
9	185.5
10	249.0
11	419.5
12	590.0
13	1042.0
14	1494.0
15	1870.0
16	2246.0
17	2191.5
18	2137.0
19	2059.0
20	1981.0
21	2392.0
22	2803.0
23	3479.5
24	4156.0
25	5357.5
26	6559.0
27	8253.0
28	9947.0
29	11759.0
30	13571.0
31	15709.5
32	17848.0
33	20789.5
34	23731.0
35	27980.5
36	32230.0
37	39046.0
38	45862.0
39	51692.0
40	57522.0
41	64215.5
42	70909.0
43	75316.5
44	79724.0
45	88725.0
46	97726.0
47	105129.5
48	112533.0
49	118187.5
50	123842.0
51	122873.0
52	121904.0
53	121585.0
54	121266.0
55	120883.5
56	120501.0
57	115953.0
58	111405.0
59	102679.0
60	93953.0
61	82452.5
62	70952.0
63	61308.0
64	51664.0
65	43372.0
66	35080.0
67	29551.0
68	24022.0
69	21268.5
70	18515.0
71	14376.5
72	10238.0
73	8928.0
74	7618.0
75	5629.5
76	3641.0
77	3252.5
78	2864.0
79	2390.0
80	1916.0
81	1523.5
82	1131.0
83	933.0
84	735.0
85	570.0
86	405.0
87	284.0
88	163.0
89	100.0
90	37.0
91	32.0
92	27.0
93	20.5
94	14.0
95	13.0
96	12.0
97	12.0
98	12.0
99	9.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03093334395422396
2	0.004845781349052252
3	1.327611328507466E-4
4	0.0
5	0.0
6	2.655222657014932E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	6.63805664253733E-5
13	0.0
14	0.0
15	0.0
16	3.319028321268665E-4
17	0.0
18	6.63805664253733E-5
19	0.0
20	1.327611328507466E-4
21	0.0
22	0.0
23	0.0
24	9.957084963805997E-4
25	1.9914169927611993E-4
26	2.655222657014932E-4
27	4.646639649776132E-4
28	0.0018586558599104528
29	2.655222657014932E-4
30	8.629473635298529E-4
31	1.9914169927611993E-4
32	3.9828339855223986E-4
33	6.63805664253733E-5
34	7.301862306791064E-4
35	6.63805664253733E-5
36	1.327611328507466E-4
37	0.0016595141606343326
38	0.0
39	3.319028321268665E-4
40	1.9914169927611993E-4
41	0.0012612307620820929
42	0.005310445314029864
43	0.004181975684798518
44	0.004447497950500011
45	0.0021241781256119458
46	0.0024560809577388125
47	0.0012612307620820929
48	0.004447497950500011
49	0.0031198866219925457
50	0.0018586558599104528
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1506465.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.12186766996704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69234930793827	38.69534264949341
2	13.738575175199319	14.046832441608675
3	4.252584042513541	6.522001160303723
4	1.8695163236423384	3.822926644163476
5	1.0248250484662396	2.6195485256279327
6	0.6595818118554385	2.0231432461914496
7	0.48420100970060864	1.7327281960505259
8	0.3438011080600565	1.4060603800827396
9	0.25950770342532814	1.1939866626462012
>10	1.50010381733096	13.86207702086618
>50	0.09977953252832167	3.497640108728835
>100	0.06643088127355222	6.89932418120822
>500	0.006786572827481461	2.3802909058864343
>1k	0.0019576652386965755	1.2980978771422376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1700	0.11284696292313463	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.327611328507466E-4
2	0.0	0.0	0.0	0.0	1.327611328507466E-4
3	0.0	0.0	0.0	0.0	1.327611328507466E-4
4	0.0	0.0	0.0	0.0	1.327611328507466E-4
5	0.0	1.327611328507466E-4	0.0	0.0	1.327611328507466E-4
6	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
7	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
8	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
9	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
10	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
11	0.0	1.327611328507466E-4	0.0	0.0	2.655222657014932E-4
12	0.0	1.327611328507466E-4	0.0	0.0	7.301862306791064E-4
13	0.0	1.327611328507466E-4	0.0	0.0	8.62947363529853E-4
14	0.0	1.327611328507466E-4	0.0	0.0	8.62947363529853E-4
15	0.0	1.327611328507466E-4	0.0	0.0	9.957084963805997E-4
16	0.0	1.327611328507466E-4	0.0	0.0	9.957084963805997E-4
17	0.0	1.327611328507466E-4	0.0	1.327611328507466E-4	0.0010620890628059729
18	0.0	1.327611328507466E-4	0.0	1.9914169927611993E-4	0.0012612307620820929
19	0.0	1.327611328507466E-4	0.0	1.9914169927611993E-4	0.0012612307620820929
20	0.0	1.327611328507466E-4	0.0	1.9914169927611993E-4	0.0012612307620820929
21	0.0	1.327611328507466E-4	0.0	2.655222657014932E-4	0.001327611328507466
22	0.0	1.327611328507466E-4	0.0	4.6466396497761315E-4	0.001327611328507466
23	0.0	1.327611328507466E-4	0.0	5.310445314029864E-4	0.001327611328507466
24	0.0	1.327611328507466E-4	0.0	7.301862306791064E-4	0.001327611328507466
25	0.0	1.327611328507466E-4	0.0	7.965667971044797E-4	0.001327611328507466
26	0.0	1.327611328507466E-4	0.0	0.0010620890628059729	0.0013939918949328394
27	0.0	1.327611328507466E-4	0.0	0.0011948501956567195	0.0013939918949328394
28	0.0	1.327611328507466E-4	0.0	0.0017922752934850794	0.0013939918949328394
29	0.0	1.327611328507466E-4	0.0	0.002787983789865679	0.0013939918949328394
30	0.0	1.327611328507466E-4	0.0	0.005376825880455238	0.0013939918949328394
31	0.0	1.327611328507466E-4	0.0	0.008961376467425397	0.0014603724613582128
32	0.0	1.327611328507466E-4	0.0	0.013010591019373168	0.001526753027783586
33	0.0	1.327611328507466E-4	0.0	0.016927044438470193	0.001526753027783586
34	0.0	1.327611328507466E-4	0.0	0.02183920635394782	0.001526753027783586
35	0.0	1.327611328507466E-4	0.0	0.027747076765806043	0.001526753027783586
36	0.0	1.327611328507466E-4	0.0	0.03484979737332099	0.001526753027783586
37	0.0	1.327611328507466E-4	0.0	0.04606811309920907	0.001526753027783586
38	0.0	1.327611328507466E-4	0.0	0.05841489845432851	0.001526753027783586
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1715	0.0	25.155497	1
GGTATCA	900	0.0	23.72303	1
CTATACA	180	0.0	20.776491	4
ACTATAC	110	1.2669807E-7	19.998762	3
GTATTAT	155	3.274181E-11	19.880955	1
GTGTACG	110	2.7839615E-6	18.009048	1
TATACAG	240	0.0	17.415588	5
TATACTG	165	1.6607373E-9	17.332262	5
GTATAGA	230	1.8189894E-12	16.269043	1
GTATAAC	110	5.598602E-5	16.008043	1
CGTCGTA	330	0.0	15.999011	10
ATACCGT	345	0.0	15.941043	6
CAACGCA	2740	0.0	15.896828	5
TCAACGC	2730	0.0	15.8744755	4
TATCAAC	2735	0.0	15.846507	2
ATAATAC	125	9.448815E-6	15.83902	3
TCTACAC	475	0.0	15.746394	3
ATCAACG	2755	0.0	15.730425	3
AACGCAG	2775	0.0	15.696327	6
ATACGAA	425	0.0	15.531028	40
>>END_MODULE