FastQCFastQC Report
Thu 2 Feb 2017
SRR4062015_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062015_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339455
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24760.18485130146216186No Hit
TATCAACGCAGAGTACTTTTTTTTT14970.11176187329921497No Hit
GCGCAAGACGGACCAGAGCGAAAGC14750.11011941423937348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4850.014.6869081
GTATCAA16600.013.102021
CGTCGTA2400.012.66716510
CGGTCCA1551.8189894E-1212.25854710
TACCGTC2750.011.7459177
CCATGCG658.019493E-411.6927689
ACCGTCG2600.011.6927688
CGCGTAA1406.7848305E-1011.53616810
CCGTCGT2650.011.472159
CGCAAGA2650.011.4700062
TACGCTA1001.927816E-611.4004489
ATACCGT3000.011.4004486
CAAGACG2500.011.3995964
GCGTAAC1354.7202775E-911.25970211
AACGAAC855.3171905E-511.17732714
AACTGAT600.005873215411.0837697
GACCGCG2150.011.04694757
AAGACGG2600.010.961155
CGGTTCT2350.010.9157312
TTAGGGG700.001491607910.8567584
Produced by FastQC (version 0.11.5)