##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062015_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1339455
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24240232034671	32.0	32.0	32.0	32.0	32.0
2	30.79303970644777	32.0	32.0	32.0	32.0	32.0
3	30.812617818441083	32.0	32.0	32.0	32.0	32.0
4	30.81979013852649	32.0	32.0	32.0	32.0	32.0
5	30.73969487590102	32.0	32.0	32.0	32.0	32.0
6	34.37195426498091	36.0	36.0	36.0	32.0	36.0
7	34.308357503611546	36.0	36.0	36.0	32.0	36.0
8	34.274026376399355	36.0	36.0	36.0	32.0	36.0
9	34.4051379105681	36.0	36.0	36.0	32.0	36.0
10	34.10815891537976	36.0	36.0	36.0	32.0	36.0
11	34.379434172853884	36.0	36.0	36.0	32.0	36.0
12	34.214676864844286	36.0	36.0	36.0	32.0	36.0
13	34.30187725604817	36.0	36.0	36.0	32.0	36.0
14	34.20790396093934	36.0	36.0	36.0	32.0	36.0
15	34.15734757793282	36.0	36.0	36.0	32.0	36.0
16	34.154317987539706	36.0	36.0	36.0	32.0	36.0
17	34.097056638707535	36.0	36.0	36.0	32.0	36.0
18	34.089473703857166	36.0	36.0	36.0	32.0	36.0
19	34.10637535415523	36.0	36.0	36.0	32.0	36.0
20	34.081094176362775	36.0	36.0	36.0	32.0	36.0
21	34.07755094422732	36.0	36.0	36.0	32.0	36.0
22	34.04681530921158	36.0	36.0	36.0	32.0	36.0
23	34.00991970614914	36.0	36.0	36.0	32.0	36.0
24	33.98166194459687	36.0	36.0	36.0	32.0	36.0
25	33.62998458328201	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	99.0
6	332.0
7	69.0
8	214.0
9	173.0
10	98.0
11	26.0
12	52.0
13	68.0
14	241.0
15	324.0
16	541.0
17	737.0
18	993.0
19	1321.0
20	1872.0
21	2655.0
22	3981.0
23	5713.0
24	8250.0
25	11398.0
26	15712.0
27	20074.0
28	26747.0
29	35028.0
30	46049.0
31	62341.0
32	89362.0
33	125748.0
34	277030.0
35	602185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.639527504737046	16.933319994445448	11.742277754467906	25.6848747463496
2	16.912364160865785	19.02427261297904	37.08785383873405	26.975509387421127
3	19.483235270622473	22.25101515623215	27.772166017519144	30.49358355562623
4	13.188633037660901	14.992608189230355	34.35220858346027	37.46655018964848
5	15.606340671035678	35.7764708078941	32.83870124535559	15.778487275714633
6	35.93262301670902	33.924342464054604	16.006777442591204	14.136257076645167
7	31.62047832585949	29.657249626307923	19.505680119581463	19.21659192825112
8	28.613389856857562	32.878439544144555	18.498050775504346	20.010119823493536
9	26.906612270159684	14.616420160692941	18.161113025486777	40.31585454366059
10	16.631591538608184	26.395610064256637	30.426620713887882	26.546177683247297
11	37.73903291527262	21.20408964318424	21.356012037158184	19.700865404384967
12	24.856257499243068	23.17911836936085	27.983194219648862	23.981429911747227
13	29.418620210705416	19.31670624962155	24.75964287876851	26.505030660904527
14	23.70141591690019	19.214851169142385	24.38526872373139	32.69846419022604
15	25.352918177108368	26.895792792193372	21.621999457250233	26.129289573448023
16	26.324834113090883	25.760254266490957	22.819251477602556	25.0956601428156
17	24.456084194914908	25.834739297912247	24.59317975739916	25.11599674977369
18	25.208925203992173	25.062653269742043	25.26692572569789	24.4614958005679
19	25.765546145010415	24.95857923603446	24.66741947706332	24.608455141891806
20	25.91060351186077	24.420711862861516	24.365336946278855	25.303347678998854
21	26.646151040938836	24.186517826250387	24.27903243765539	24.888298695155388
22	26.21431710357569	23.987022770687787	24.659874622207475	25.138785503529053
23	24.67160222249454	24.27969179177414	25.186577758914936	25.862128226816388
24	24.990939003238044	24.777659953682427	24.876153922608253	25.355247120471276
25	25.010424341697217	24.38765399256023	24.901697336898284	25.700224328844268
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	327.0
1	327.0
2	371.0
3	415.0
4	415.0
5	415.0
6	862.0
7	1309.0
8	1309.0
9	1309.0
10	1619.5
11	1930.0
12	1930.0
13	1930.0
14	2555.0
15	3180.0
16	3180.0
17	3180.0
18	5155.5
19	7131.0
20	7131.0
21	7131.0
22	11358.0
23	15585.0
24	15585.0
25	15585.0
26	23020.0
27	30455.0
28	30455.0
29	30455.0
30	38848.5
31	47242.0
32	47242.0
33	47242.0
34	60601.5
35	73961.0
36	73961.0
37	73961.0
38	88410.0
39	102859.0
40	102859.0
41	102859.0
42	120905.5
43	138952.0
44	138952.0
45	138952.0
46	154895.5
47	170839.0
48	170839.0
49	170839.0
50	177819.5
51	184800.0
52	184800.0
53	184800.0
54	176472.5
55	168145.0
56	168145.0
57	168145.0
58	155771.0
59	143397.0
60	143397.0
61	143397.0
62	126092.0
63	108787.0
64	108787.0
65	108787.0
66	88625.5
67	68464.0
68	68464.0
69	68464.0
70	52341.0
71	36218.0
72	36218.0
73	36218.0
74	27031.5
75	17845.0
76	17845.0
77	17845.0
78	14244.0
79	10643.0
80	10643.0
81	10643.0
82	7259.5
83	3876.0
84	3876.0
85	3876.0
86	2841.5
87	1807.0
88	1807.0
89	1807.0
90	1204.0
91	601.0
92	601.0
93	601.0
94	377.5
95	154.0
96	154.0
97	154.0
98	343.5
99	533.0
100	533.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.705439899063425E-4
2	2.2397168997838676E-4
3	4.479433799567735E-4
4	0.010078726049027403
5	0.03583547039654188
6	0.06696753530353763
7	0.10862626963951756
8	0.11101530099928703
9	0.12124334150830003
10	0.1381158754866718
11	0.14408845388609548
12	0.13587615858688795
13	0.1312474103273346
14	0.11900362460851616
15	0.13602547304687354
16	0.12288580056814152
17	0.12706660544773807
18	0.11467350526893402
19	0.1013845183302164
20	0.09735302791060543
21	0.09615851223072071
22	0.10593860935977693
23	0.08503458496179415
24	0.09720371345061984
25	0.09264962242105931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1339455.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.16908419625947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.546489258141	44.118843684611114
2	12.560944199441426	14.110734646458848
3	3.8011934269366545	6.405286609316191
4	1.6687330873196595	3.7492483713096783
5	0.9046634748431327	2.540705944387229
6	0.5252850274581874	1.7702867360600036
7	0.3613489468411471	1.4207647593545054
8	0.25896687223208364	1.16367456403567
9	0.1930892527253097	0.9761081844348669
>10	1.0308302332922454	10.429072122745872
>50	0.07897186446484021	3.074936722811197
>100	0.06201581464653016	6.968078900070836
>500	0.005868139873993804	2.1320396595643705
>1k	0.001600401783816492	1.1402190948395798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2476	0.18485130146216186	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1497	0.11176187329921497	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1475	0.11011941423937348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4931445998559115E-4
2	0.0	0.0	0.0	0.0	1.4931445998559115E-4
3	0.0	0.0	0.0	0.0	1.4931445998559115E-4
4	0.0	0.0	0.0	0.0	1.4931445998559115E-4
5	0.0	0.0	0.0	0.0	1.4931445998559115E-4
6	0.0	0.0	0.0	0.0	1.4931445998559115E-4
7	0.0	0.0	0.0	0.0	1.4931445998559115E-4
8	0.0	0.0	0.0	0.0	1.4931445998559115E-4
9	0.0	0.0	0.0	0.0	1.4931445998559115E-4
10	0.0	0.0	0.0	0.0	1.4931445998559115E-4
11	0.0	0.0	0.0	0.0	1.4931445998559115E-4
12	0.0	0.0	0.0	0.0	2.986289199711823E-4
13	0.0	0.0	0.0	0.0	4.4794337995677347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	485	0.0	14.686908	1
GTATCAA	1660	0.0	13.10202	1
CGTCGTA	240	0.0	12.667165	10
CGGTCCA	155	1.8189894E-12	12.258547	10
TACCGTC	275	0.0	11.745917	7
CCATGCG	65	8.019493E-4	11.692768	9
ACCGTCG	260	0.0	11.692768	8
CGCGTAA	140	6.7848305E-10	11.536168	10
CCGTCGT	265	0.0	11.47215	9
CGCAAGA	265	0.0	11.470006	2
TACGCTA	100	1.927816E-6	11.400448	9
ATACCGT	300	0.0	11.400448	6
CAAGACG	250	0.0	11.399596	4
GCGTAAC	135	4.7202775E-9	11.259702	11
AACGAAC	85	5.3171905E-5	11.177327	14
AACTGAT	60	0.0058732154	11.083769	7
GACCGCG	215	0.0	11.0469475	7
AAGACGG	260	0.0	10.96115	5
CGGTTCT	235	0.0	10.91573	12
TTAGGGG	70	0.0014916079	10.856758	4
>>END_MODULE