FastQCFastQC Report
Thu 2 Feb 2017
SRR4062015_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062015_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339455
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13750.10265369124009394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15450.026.9201351
GGTATCA6100.026.6959761
TTAGTAC856.1172505E-620.7047063
AACGAAT654.933194E-420.30729731
CGCAATA3500.019.48606136
CTAGCGG3500.019.48533429
CTATAGG3100.019.166641
GCGCAAT3700.019.02665735
CGCCGGT3700.019.0259467
TATAGGG2900.018.9658552
TCTAGCG3600.018.94407528
TACGAAT3500.018.85888941
GTCCTAA1801.4551915E-1118.3384531
CCGTCGT3250.018.2758859
GTATAGG1701.2551027E-1018.1227071
TAGCGGC3800.017.94701830
TCAACGC23350.017.9004824
ACCGTCG3200.017.8739838
ATACGAA3700.017.83948940
CAATACG3700.017.83948938
Produced by FastQC (version 0.11.5)