##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062015_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1339455
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.271739625444678	32.0	32.0	32.0	32.0	32.0
2	31.427517161830746	32.0	32.0	32.0	32.0	32.0
3	31.557650686286586	32.0	32.0	32.0	32.0	32.0
4	31.63532182865419	32.0	32.0	32.0	32.0	32.0
5	31.60330059613798	32.0	32.0	32.0	32.0	32.0
6	35.234446845918676	36.0	36.0	36.0	36.0	36.0
7	35.241918541496354	36.0	36.0	36.0	36.0	36.0
8	35.18715447700744	36.0	36.0	36.0	36.0	36.0
9	35.273538118115205	36.0	36.0	36.0	36.0	36.0
10	35.15975975303388	36.0	36.0	36.0	36.0	36.0
11	35.2818370158012	36.0	36.0	36.0	36.0	36.0
12	35.20075403802293	36.0	36.0	36.0	36.0	36.0
13	35.234136271841905	36.0	36.0	36.0	36.0	36.0
14	35.19543993639204	36.0	36.0	36.0	36.0	36.0
15	35.17347876561736	36.0	36.0	36.0	36.0	36.0
16	35.18631607631462	36.0	36.0	36.0	36.0	36.0
17	35.16561735929912	36.0	36.0	36.0	36.0	36.0
18	35.16203157254257	36.0	36.0	36.0	36.0	36.0
19	35.15830543019362	36.0	36.0	36.0	36.0	36.0
20	35.154062659813135	36.0	36.0	36.0	36.0	36.0
21	35.151162226427914	36.0	36.0	36.0	36.0	36.0
22	35.13553870790732	36.0	36.0	36.0	36.0	36.0
23	35.10095822554696	36.0	36.0	36.0	36.0	36.0
24	35.08660835937004	36.0	36.0	36.0	36.0	36.0
25	35.06714596608322	36.0	36.0	36.0	36.0	36.0
26	35.02564550507483	36.0	36.0	36.0	36.0	36.0
27	35.015409252270516	36.0	36.0	36.0	36.0	36.0
28	34.997936474163	36.0	36.0	36.0	36.0	36.0
29	34.97595215964702	36.0	36.0	36.0	36.0	36.0
30	34.960993090473366	36.0	36.0	36.0	36.0	36.0
31	34.96411674897626	36.0	36.0	36.0	36.0	36.0
32	34.93449723954892	36.0	36.0	36.0	32.0	36.0
33	34.91859972899425	36.0	36.0	36.0	32.0	36.0
34	34.904401416994226	36.0	36.0	36.0	32.0	36.0
35	34.89258989663706	36.0	36.0	36.0	32.0	36.0
36	34.868864575517655	36.0	36.0	36.0	32.0	36.0
37	34.85372856870892	36.0	36.0	36.0	32.0	36.0
38	34.84062323855598	36.0	36.0	36.0	32.0	36.0
39	34.83200107506411	36.0	36.0	36.0	32.0	36.0
40	34.81570489490129	36.0	36.0	36.0	32.0	36.0
41	34.79291652201828	36.0	36.0	36.0	32.0	36.0
42	34.7658390912722	36.0	36.0	36.0	32.0	36.0
43	34.76101399449776	36.0	36.0	36.0	32.0	36.0
44	34.71439503380106	36.0	36.0	36.0	32.0	36.0
45	34.70032289251972	36.0	36.0	36.0	32.0	36.0
46	34.681288285160754	36.0	36.0	36.0	32.0	36.0
47	34.66382446592084	36.0	36.0	36.0	32.0	36.0
48	34.6393503327846	36.0	36.0	36.0	32.0	36.0
49	34.61881212881359	36.0	36.0	36.0	32.0	36.0
50	34.19885550466421	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	11.0
21	34.0
22	96.0
23	243.0
24	622.0
25	1517.0
26	3280.0
27	6130.0
28	10478.0
29	16706.0
30	25083.0
31	37337.0
32	57294.0
33	97875.0
34	224312.0
35	858432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.1244715418618	17.409899801125732	12.231252758512275	26.23437589850019
2	16.407144766333232	19.43509936642611	36.83329525618455	27.32446061105611
3	18.786325462707538	22.620726520452752	28.104009621838166	30.48893839500154
4	12.949455524415171	15.400304900354922	34.36758559831095	37.28265397691896
5	15.239631043969377	36.10013027686634	32.64775599030949	16.012482688854796
6	36.163595261345684	34.08699975811003	15.82341136524489	13.925993615299392
7	31.701550257380802	29.66557293824727	19.449402928803135	19.183473875568797
8	28.70577958945989	32.91226655617396	18.432272827381286	19.949681026984855
9	26.981496205546286	14.37875852492245	18.019940946131076	40.61980432340019
10	16.559284604025223	26.509980932529224	30.46756364906895	26.463170814376603
11	38.02180737688089	21.108659865393015	21.288359817985672	19.581172939740416
12	24.920434057135175	23.046089640936053	28.00146328170786	24.03201302022091
13	29.60599646871302	19.108592673886022	24.753127204721324	26.532283652679634
14	23.765859995296594	19.071413373349607	24.4621879794394	32.70053865191439
15	25.41197725940774	26.791418897984627	21.656121332930184	26.140482509677444
16	26.40117839939557	25.72731670752939	22.73137212156686	25.140132771508178
17	24.689817873687435	25.716728072238336	24.545878734261322	25.04757531981291
18	25.300775764435183	24.965489643906878	25.356470141169567	24.37726445048837
19	25.82709073995822	24.747695702875948	24.775318898595994	24.649894658569835
20	25.965209600955617	24.20403897121953	24.446899846952107	25.383851580872747
21	26.848606336159108	23.996700150434318	24.355726769469673	24.7989667439369
22	26.32943995878921	23.81647759723171	24.698328797906612	25.15575364607247
23	24.844544750730336	24.082815895742517	25.185728801234536	25.886910552292612
24	25.218953933630324	24.53889208674988	24.987289435785335	25.25486454383446
25	25.286759919011416	24.046024929672523	24.98350066594597	25.68371448537009
26	25.154541273718728	24.81231074293291	25.163425530517046	24.869722452831315
27	25.46364283170592	24.437622391927416	24.65188992173636	25.446844854630307
28	25.167720597567623	24.33811397385455	25.138454417177456	25.355711011400373
29	25.10244930168734	24.620009959363706	25.08945882728866	25.188081911660294
30	25.07855947786784	24.72228879277843	25.06019347021235	25.13895825914138
31	25.53322378563691	24.415971665918846	24.399696292574475	25.651108255869776
32	25.50375605291174	24.225234762639257	24.527301748942467	25.743707435506536
33	25.015192803016163	24.176266377990967	24.928814065474636	25.879726753518234
34	25.716920067639954	24.25288085737003	24.946078413074073	25.084120661915943
35	26.068123381800916	24.245512343854053	24.865616685032386	24.820747589312646
36	24.92985927059614	24.870207921161672	24.780320280712232	25.419612527529956
37	25.96442215302192	24.37755192697784	24.482820664626495	25.175205255373744
38	25.316042718867003	24.148926242389628	24.9440257418129	25.59100529693047
39	25.718786512324133	24.166875086323067	24.58473472221704	25.52960367913576
40	25.791274497873385	24.333291276364307	24.8819480788053	24.993486146957004
41	24.962054952939184	24.793327345743023	25.14093607986042	25.103681621457376
42	26.171044684074214	24.60390284765873	24.61219025803711	24.61286221022995
43	25.658187975633812	23.973325309822254	24.782271425126382	25.586215289417552
44	25.181276280581038	24.598175888761467	24.646033424120795	25.574514406536696
45	25.261959422886687	24.803777740448325	24.567706291878977	25.366556544786008
46	25.24803590799919	24.485618060911303	24.746775290554215	25.51957074053529
47	25.37198436956395	24.46712725784993	25.04050206131228	25.120386311273833
48	26.07568607160962	24.874402811405993	24.079943198489474	24.96996791849491
49	25.364210771515367	25.069900545242916	24.24699660968357	25.318892073558146
50	25.533659043534872	24.858372802799995	24.32889463593523	25.279073517729906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	152.0
1	104.5
2	57.0
3	123.5
4	190.0
5	206.0
6	222.0
7	172.5
8	123.0
9	176.0
10	229.0
11	429.0
12	629.0
13	1109.0
14	1589.0
15	2056.0
16	2523.0
17	2411.0
18	2299.0
19	2168.5
20	2038.0
21	2430.5
22	2823.0
23	3528.5
24	4234.0
25	5447.5
26	6661.0
27	8215.5
28	9770.0
29	11373.5
30	12977.0
31	15049.5
32	17122.0
33	19866.5
34	22611.0
35	26616.5
36	30622.0
37	36186.5
38	41751.0
39	47319.0
40	52887.0
41	57994.5
42	63102.0
43	67563.5
44	72025.0
45	79944.5
46	87864.0
47	93745.0
48	99626.0
49	103612.5
50	107599.0
51	105183.0
52	102767.0
53	102786.5
54	102806.0
55	103328.5
56	103851.0
57	100555.0
58	97259.0
59	89750.0
60	82241.0
61	72676.5
62	63112.0
63	55240.5
64	47369.0
65	39614.5
66	31860.0
67	27189.5
68	22519.0
69	19868.0
70	17217.0
71	13309.0
72	9401.0
73	8358.0
74	7315.0
75	5477.0
76	3639.0
77	3094.0
78	2549.0
79	2092.5
80	1636.0
81	1293.5
82	951.0
83	826.5
84	702.0
85	505.5
86	309.0
87	220.0
88	131.0
89	85.5
90	40.0
91	29.0
92	18.0
93	14.5
94	11.0
95	10.5
96	10.0
97	10.5
98	11.0
99	8.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031206722136988548
2	0.005151348869502895
3	1.4931445998559115E-4
4	7.465722999279557E-5
5	0.0
6	2.2397168997838676E-4
7	0.0
8	0.0
9	0.0
10	7.465722999279557E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.212295299207513E-4
17	0.0
18	1.4931445998559115E-4
19	7.465722999279557E-5
20	3.732861499639779E-4
21	0.0
22	0.0
23	1.4931445998559115E-4
24	0.0013438301398703203
25	5.226006099495691E-4
26	5.226006099495691E-4
27	5.972578399423646E-4
28	0.0018664307498198894
29	8.95886759913547E-4
30	0.001791773519827094
31	5.972578399423646E-4
32	0.0012691729098775247
33	3.732861499639779E-4
34	7.465722999279558E-4
35	2.2397168997838676E-4
36	3.732861499639779E-4
37	0.001941087979812685
38	0.0
39	7.465722999279558E-4
40	1.4931445998559115E-4
41	0.0013438301398703203
42	0.0055246350194668725
43	0.004330119339582144
44	0.0047034054895461214
45	0.0022397168997838673
46	0.0023890313597694584
47	0.0012691729098775247
48	0.004031490419610962
49	0.00388217595962537
50	0.0017171162898342984
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1339455.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.34929384783377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21307401565636	44.85464564466248
2	13.056497405938087	14.975618127132453
3	3.809618319525631	6.554367613636983
4	1.5855744224133068	3.6372629387436017
5	0.8610999346110457	2.4691736591179656
6	0.5465723522543132	1.8807323063120593
7	0.36570650520779724	1.468110688045842
8	0.24948135912146868	1.1446063819051242
9	0.18667553785642646	0.9635139247257556
>10	0.9928604877729702	10.194180137097444
>50	0.07153706279021084	2.854866220038975
>100	0.05514616745857481	6.334262602455578
>500	0.004846550799415217	1.8178436998869425
>1k	0.0013098785944365453	0.8508160562386673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1375	0.10265369124009394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	7.465722999279557E-5	0.0	0.0	0.0	0.0
3	7.465722999279557E-5	0.0	0.0	0.0	0.0
4	7.465722999279557E-5	0.0	0.0	0.0	0.0
5	7.465722999279557E-5	0.0	0.0	0.0	0.0
6	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
7	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
8	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
9	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
10	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
11	7.465722999279557E-5	0.0	0.0	0.0	7.465722999279557E-5
12	7.465722999279557E-5	0.0	0.0	0.0	2.2397168997838674E-4
13	7.465722999279557E-5	0.0	0.0	0.0	2.2397168997838674E-4
14	7.465722999279557E-5	0.0	0.0	0.0	2.2397168997838674E-4
15	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
16	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
17	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
18	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
19	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
20	7.465722999279557E-5	0.0	0.0	1.4931445998559115E-4	3.732861499639779E-4
21	7.465722999279557E-5	0.0	0.0	2.986289199711823E-4	3.732861499639779E-4
22	7.465722999279557E-5	0.0	0.0	3.732861499639779E-4	3.732861499639779E-4
23	7.465722999279557E-5	0.0	0.0	3.732861499639779E-4	3.732861499639779E-4
24	1.4931445998559115E-4	0.0	0.0	8.212295299207513E-4	3.732861499639779E-4
25	1.4931445998559115E-4	0.0	0.0	9.705439899063425E-4	3.732861499639779E-4
26	1.4931445998559115E-4	0.0	0.0	0.001567801829848707	4.4794337995677347E-4
27	1.4931445998559115E-4	0.0	0.0	0.0017171162898342984	5.226006099495691E-4
28	2.2397168997838674E-4	0.0	0.0	0.002314374129776663	5.972578399423646E-4
29	2.2397168997838674E-4	0.0	0.0	0.0035835470396541878	5.972578399423646E-4
30	2.2397168997838674E-4	0.0	0.0	0.005449977789474077	5.972578399423646E-4
31	2.2397168997838674E-4	0.0	0.0	0.00910818205912106	5.972578399423646E-4
32	2.2397168997838674E-4	0.0	0.0	0.013587615858688794	6.719150699351602E-4
33	2.2397168997838674E-4	0.0	0.0	0.018664307498198893	6.719150699351602E-4
34	2.2397168997838674E-4	0.0	0.0	0.02396497082768738	6.719150699351602E-4
35	2.986289199711823E-4	0.0	0.0	0.029414948617161457	6.719150699351602E-4
36	2.986289199711823E-4	0.0	0.0	0.036208756546505856	6.719150699351602E-4
37	2.986289199711823E-4	0.0	0.0	0.04778062719538917	6.719150699351602E-4
38	2.986289199711823E-4	0.0	0.0	0.06457850394376817	7.465722999279558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1545	0.0	26.920135	1
GGTATCA	610	0.0	26.695976	1
TTAGTAC	85	6.1172505E-6	20.704706	3
AACGAAT	65	4.933194E-4	20.307297	31
CGCAATA	350	0.0	19.486061	36
CTAGCGG	350	0.0	19.485334	29
CTATAGG	310	0.0	19.16664	1
GCGCAAT	370	0.0	19.026657	35
CGCCGGT	370	0.0	19.025946	7
TATAGGG	290	0.0	18.965855	2
TCTAGCG	360	0.0	18.944075	28
TACGAAT	350	0.0	18.858889	41
GTCCTAA	180	1.4551915E-11	18.338453	1
CCGTCGT	325	0.0	18.275885	9
GTATAGG	170	1.2551027E-10	18.122707	1
TAGCGGC	380	0.0	17.947018	30
TCAACGC	2335	0.0	17.900482	4
ACCGTCG	320	0.0	17.873983	8
ATACGAA	370	0.0	17.839489	40
CAATACG	370	0.0	17.839489	38
>>END_MODULE