FastQCFastQC Report
Thu 2 Feb 2017
SRR4062014_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062014_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590064
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAC307.717742E-418.9989817
CTAGTAC250.006035512318.995763
GTATTAG250.00604142818.9925381
GTACATA350.002173747916.279321
GGTATCA2850.015.3273121
GCATTTA456.77367E-414.7719751
AGTACTG602.5642255E-514.2492375
ACACCTA400.00527583714.2492366
GTGTTAG655.4613927E-513.1486811
ATTAACC550.003069690712.0882113
TCTAGAA550.003069690712.0882112
GTCCTAA550.00307359312.0861611
GGCTAGG550.00307359312.0861611
TAGGCTG951.0368003E-611.9993585
TAGGGTG658.0140674E-411.6916815
TTAAGAC658.025482E-411.6896983
TCAGTAC658.025482E-411.6896983
TAGACAG752.0706862E-411.3993885
CTAGGAC752.0739519E-411.3974553
GTGGTAT855.3309363E-511.1720811
Produced by FastQC (version 0.11.5)