##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062014_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	590064
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.343383090647794	32.0	32.0	32.0	32.0	32.0
2	30.959885707313106	32.0	32.0	32.0	32.0	32.0
3	30.988697836166924	32.0	32.0	32.0	32.0	32.0
4	30.991626332058896	32.0	32.0	32.0	32.0	32.0
5	30.936391645651998	32.0	32.0	32.0	32.0	32.0
6	34.575322337915885	36.0	36.0	36.0	32.0	36.0
7	34.53698242902465	36.0	36.0	36.0	32.0	36.0
8	34.497388418883375	36.0	36.0	36.0	32.0	36.0
9	34.613048754033464	36.0	36.0	36.0	32.0	36.0
10	34.375716871390225	36.0	36.0	36.0	32.0	36.0
11	34.578244054882184	36.0	36.0	36.0	32.0	36.0
12	34.4558285202961	36.0	36.0	36.0	32.0	36.0
13	34.50875667724179	36.0	36.0	36.0	32.0	36.0
14	34.44358239106266	36.0	36.0	36.0	32.0	36.0
15	34.40197673472708	36.0	36.0	36.0	32.0	36.0
16	34.38890188182977	36.0	36.0	36.0	32.0	36.0
17	34.34833679058543	36.0	36.0	36.0	32.0	36.0
18	34.33296727134684	36.0	36.0	36.0	32.0	36.0
19	34.353036280810215	36.0	36.0	36.0	32.0	36.0
20	34.32276837766751	36.0	36.0	36.0	32.0	36.0
21	34.317497762954524	36.0	36.0	36.0	32.0	36.0
22	34.29969799886114	36.0	36.0	36.0	32.0	36.0
23	34.26168686786518	36.0	36.0	36.0	32.0	36.0
24	34.23587610835435	36.0	36.0	36.0	32.0	36.0
25	33.881324737655575	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	16.0
5	36.0
6	116.0
7	19.0
8	104.0
9	92.0
10	44.0
11	14.0
12	34.0
13	31.0
14	53.0
15	81.0
16	152.0
17	202.0
18	268.0
19	371.0
20	570.0
21	859.0
22	1263.0
23	1900.0
24	2742.0
25	3975.0
26	5762.0
27	7459.0
28	10091.0
29	13691.0
30	18377.0
31	25019.0
32	36107.0
33	51943.0
34	119144.0
35	289529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18905672464372	16.86605283182586	11.977575199852218	25.967315243678197
2	16.988157216844275	18.470708262154613	38.081462349846795	26.459672171154313
3	20.292038592750895	21.017050837289155	27.619780394841868	31.071130175118082
4	13.843465468030725	14.881763513467705	33.40316336838394	37.871607650117625
5	15.706322538644892	35.33748800558779	33.180189130265255	15.776000325502071
6	36.44242982317798	32.81882483817741	15.921104175251703	14.817641163392908
7	32.08725066754429	29.347180706867476	18.709900691124627	19.855667934463604
8	28.413430625232216	33.59217140656466	18.013874444589597	19.98052352361353
9	26.71288543209625	15.4342510914178	18.308332103208336	39.544531373277614
10	17.35370580289568	26.959300312917872	30.340476368252073	25.346517515934373
11	37.10141090780734	21.657451812933378	20.827436166349322	20.41370111290996
12	24.704991973609637	23.416018163738364	27.792691742490316	24.086298120161683
13	30.128808819611656	19.60792298959993	24.227970060561017	26.035298130227396
14	23.5027334086675	19.260944663320746	24.546043768472988	32.690278159538764
15	25.33671590629481	26.64217412331685	21.676579641775344	26.344530328613004
16	25.772149836857206	25.919427990654288	22.458052025838153	25.850370146650352
17	24.36198950676359	26.161823895529174	24.440892400209048	25.035294197498185
18	24.941592848283925	25.49690616002457	24.874744866363418	24.686756125328092
19	26.003810144212093	25.16052310599699	24.272620398691046	24.56304635109987
20	26.16091587462872	24.759250432136355	24.56994162978594	24.509892063448984
21	26.23503647901587	24.574354938025536	24.236790457272647	24.95381812568594
22	26.151708647738992	24.32702486292818	24.263578253080723	25.2576882362521
23	25.318027314730006	24.420087792500322	24.64211326182383	25.619771630945838
24	25.084856164348707	24.755269902820395	24.45689510404104	25.702978828789863
25	25.420753199963357	24.54243831610515	24.476115195288514	25.560693288642973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	21.0
2	47.5
3	74.0
4	74.0
5	74.0
6	142.0
7	210.0
8	210.0
9	210.0
10	262.5
11	315.0
12	315.0
13	315.0
14	476.0
15	637.0
16	637.0
17	637.0
18	1279.5
19	1922.0
20	1922.0
21	1922.0
22	3338.5
23	4755.0
24	4755.0
25	4755.0
26	7544.5
27	10334.0
28	10334.0
29	10334.0
30	14731.0
31	19128.0
32	19128.0
33	19128.0
34	25013.5
35	30899.0
36	30899.0
37	30899.0
38	38854.5
39	46810.0
40	46810.0
41	46810.0
42	54625.5
43	62441.0
44	62441.0
45	62441.0
46	70174.0
47	77907.0
48	77907.0
49	77907.0
50	82287.5
51	86668.0
52	86668.0
53	86668.0
54	84059.5
55	81451.0
56	81451.0
57	81451.0
58	74243.0
59	67035.0
60	67035.0
61	67035.0
62	57499.5
63	47964.0
64	47964.0
65	47964.0
66	38443.0
67	28922.0
68	28922.0
69	28922.0
70	21043.0
71	13164.0
72	13164.0
73	13164.0
74	9493.0
75	5822.0
76	5822.0
77	5822.0
78	4054.5
79	2287.0
80	2287.0
81	2287.0
82	1514.0
83	741.0
84	741.0
85	741.0
86	498.0
87	255.0
88	255.0
89	255.0
90	159.0
91	63.0
92	63.0
93	63.0
94	53.0
95	43.0
96	43.0
97	43.0
98	119.5
99	196.0
100	196.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.084194256894167E-4
2	0.0
3	8.473657094823613E-4
4	0.010168388513788335
5	0.03491146723067328
6	0.0635524282111771
7	0.09998915371891863
8	0.10761544510425988
9	0.11744488733425526
10	0.1308332655440766
11	0.13523956723338484
12	0.12846064155752598
13	0.12557959814528594
14	0.11693646790856586
15	0.13032484611838716
16	0.11913961875322
17	0.12405433986821769
18	0.113038585644947
19	0.09914178800943627
20	0.09337970118495621
21	0.09270180861737032
22	0.10032810000271157
23	0.08338078581306435
24	0.0910070771984056
25	0.0891428726375444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	590064.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.24551855943541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.35341695062138	34.66668676775645
2	16.763721742341335	17.516586708294103
3	7.096498952064731	11.122808031213355
4	3.595859089971963	7.514700912889789
5	1.9249254640107731	5.0284364527752325
6	1.218928697676509	3.821013713825189
7	0.7514901520534716	2.7483394880439813
8	0.5137286114373167	2.147201416268905
9	0.3591089936233861	1.688565203208971
>10	1.3705656050265798	11.300662419107187
>50	0.037758910220952305	1.3501321079399509
>100	0.01399683095163208	1.0948667786768322
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.389462837929445E-4
2	0.0	0.0	0.0	0.0	3.389462837929445E-4
3	0.0	0.0	0.0	0.0	3.389462837929445E-4
4	0.0	0.0	0.0	0.0	3.389462837929445E-4
5	0.0	0.0	0.0	0.0	3.389462837929445E-4
6	0.0	0.0	0.0	0.0	5.084194256894167E-4
7	0.0	0.0	0.0	0.0	5.084194256894167E-4
8	0.0	0.0	0.0	0.0	5.084194256894167E-4
9	0.0	0.0	0.0	0.0	5.084194256894167E-4
10	0.0	0.0	0.0	0.0	5.084194256894167E-4
11	0.0	0.0	0.0	0.0	5.084194256894167E-4
12	0.0	0.0	0.0	0.0	0.0010168388513788335
13	0.0	0.0	0.0	0.0	0.0010168388513788335
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTGAC	30	7.717742E-4	18.998981	7
CTAGTAC	25	0.0060355123	18.99576	3
GTATTAG	25	0.006041428	18.992538	1
GTACATA	35	0.0021737479	16.27932	1
GGTATCA	285	0.0	15.327312	1
GCATTTA	45	6.77367E-4	14.771975	1
AGTACTG	60	2.5642255E-5	14.249237	5
ACACCTA	40	0.005275837	14.249236	6
GTGTTAG	65	5.4613927E-5	13.148681	1
ATTAACC	55	0.0030696907	12.088211	3
TCTAGAA	55	0.0030696907	12.088211	2
GTCCTAA	55	0.003073593	12.086161	1
GGCTAGG	55	0.003073593	12.086161	1
TAGGCTG	95	1.0368003E-6	11.999358	5
TAGGGTG	65	8.0140674E-4	11.691681	5
TTAAGAC	65	8.025482E-4	11.689698	3
TCAGTAC	65	8.025482E-4	11.689698	3
TAGACAG	75	2.0706862E-4	11.399388	5
CTAGGAC	75	2.0739519E-4	11.397455	3
GTGGTAT	85	5.3309363E-5	11.172081	1
>>END_MODULE