FastQCFastQC Report
Thu 2 Feb 2017
SRR4062014_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062014_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590064
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT250.002352553735.197766
TGTACCG451.0365347E-634.2200475
GTGTCGC554.957852E-627.9982211
GGTATCA2500.026.4095121
GTATAGG508.313925E-526.409511
GTATCAA5750.025.6440181
GCATTAT450.00139443724.453251
TCGTTTA450.001397884524.4428930
GTTATGA551.5883839E-424.0086481
TATACTC500.002579839221.9986023
GTATTAG755.451408E-520.5407311
ACGTGAA1504.638423E-1019.06707234
TATAAAC708.120063E-418.8559442
GAGCGAT600.00741043418.33216911
GTCCTAG1355.4886186E-817.9323841
TACGCTC1002.4790714E-517.60037234
ACACCGT750.00129035817.598886
CTTAGTT750.00129035817.5988814
CCCTAGA1651.6461854E-917.3395771
CTACGCT1154.2645424E-617.21775433
Produced by FastQC (version 0.11.5)