##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	590064
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.302716315518317	32.0	32.0	32.0	32.0	32.0
2	31.45747410450392	32.0	32.0	32.0	32.0	32.0
3	31.56927892567586	32.0	32.0	32.0	32.0	32.0
4	31.645452018764065	32.0	32.0	32.0	32.0	32.0
5	31.618768472572466	32.0	32.0	32.0	32.0	32.0
6	35.265184793513924	36.0	36.0	36.0	36.0	36.0
7	35.271799330242146	36.0	36.0	36.0	36.0	36.0
8	35.226887252908156	36.0	36.0	36.0	36.0	36.0
9	35.30240787440007	36.0	36.0	36.0	36.0	36.0
10	35.192911277420755	36.0	36.0	36.0	36.0	36.0
11	35.30465847772445	36.0	36.0	36.0	36.0	36.0
12	35.240639998373055	36.0	36.0	36.0	36.0	36.0
13	35.2584448466607	36.0	36.0	36.0	36.0	36.0
14	35.23042754684238	36.0	36.0	36.0	36.0	36.0
15	35.20064772634833	36.0	36.0	36.0	36.0	36.0
16	35.226990631524714	36.0	36.0	36.0	36.0	36.0
17	35.204906586404185	36.0	36.0	36.0	36.0	36.0
18	35.20783677702757	36.0	36.0	36.0	36.0	36.0
19	35.200573158165895	36.0	36.0	36.0	36.0	36.0
20	35.20255429919467	36.0	36.0	36.0	36.0	36.0
21	35.19285196182109	36.0	36.0	36.0	36.0	36.0
22	35.17115939965834	36.0	36.0	36.0	36.0	36.0
23	35.14369288755118	36.0	36.0	36.0	36.0	36.0
24	35.12377809864692	36.0	36.0	36.0	36.0	36.0
25	35.125413514466224	36.0	36.0	36.0	36.0	36.0
26	35.084029867946526	36.0	36.0	36.0	36.0	36.0
27	35.06644194528051	36.0	36.0	36.0	36.0	36.0
28	35.05502115024811	36.0	36.0	36.0	36.0	36.0
29	35.02378047127091	36.0	36.0	36.0	36.0	36.0
30	35.00863126711679	36.0	36.0	36.0	36.0	36.0
31	35.02169256216275	36.0	36.0	36.0	36.0	36.0
32	34.98328316928333	36.0	36.0	36.0	36.0	36.0
33	34.98305946473603	36.0	36.0	36.0	36.0	36.0
34	34.97042185254481	36.0	36.0	36.0	36.0	36.0
35	34.964668239377424	36.0	36.0	36.0	36.0	36.0
36	34.9340596952195	36.0	36.0	36.0	36.0	36.0
37	34.938681227799016	36.0	36.0	36.0	36.0	36.0
38	34.94144364001193	36.0	36.0	36.0	36.0	36.0
39	34.91751233764473	36.0	36.0	36.0	36.0	36.0
40	34.89401997071504	36.0	36.0	36.0	36.0	36.0
41	34.88450574852897	36.0	36.0	36.0	36.0	36.0
42	34.87393570866889	36.0	36.0	36.0	32.0	36.0
43	34.86339617397435	36.0	36.0	36.0	32.0	36.0
44	34.83652620732666	36.0	36.0	36.0	32.0	36.0
45	34.82309037663711	36.0	36.0	36.0	32.0	36.0
46	34.811215393584426	36.0	36.0	36.0	32.0	36.0
47	34.80200113885952	36.0	36.0	36.0	32.0	36.0
48	34.78526905556007	36.0	36.0	36.0	32.0	36.0
49	34.76686766181295	36.0	36.0	36.0	32.0	36.0
50	34.373588288728	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	5.0
22	27.0
23	91.0
24	221.0
25	615.0
26	1265.0
27	2465.0
28	4417.0
29	6837.0
30	10366.0
31	15570.0
32	23601.0
33	40000.0
34	92383.0
35	392199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75029666045092	17.459738938803188	12.400067808103069	26.38989659264282
2	16.464815944681714	18.91261609382415	37.656769032607954	26.965798928886176
3	19.665966172931565	21.460698911975054	27.79903738602854	31.074297529064843
4	13.664280484828764	15.309017326934027	33.40315626779468	37.62354592044253
5	15.377484476260204	35.76035819843271	32.75407413433119	16.108083190975893
6	36.54813130123394	32.95021040126224	15.896777123798644	14.60488117370518
7	32.12092247620597	29.34173242224572	18.677296022126413	19.86004907942189
8	28.375057620868244	33.705157406654195	18.013639198459828	19.906145774017734
9	26.781332194473823	15.337488814772634	18.21870170015456	39.662477290598986
10	17.22751168521274	27.024448278316516	30.44696997942589	25.301070057044857
11	37.49661053716207	21.319382304292418	20.826384934515577	20.357622224029935
12	24.760195504216494	23.375430461780418	27.90188860869329	23.962485425309797
13	30.153000352504133	19.416537867078826	24.252453971094663	26.17800780932238
14	23.534396268879306	19.01268336993953	24.51666259931126	32.936257761869896
15	25.534687762683372	26.55881395916375	21.645448629301228	26.261049648851646
16	25.89553996776615	25.921130455561524	22.41184415901353	25.77148541765879
17	24.504460533094715	26.019042002223486	24.431417608937338	25.04507985574446
18	25.189979392065947	25.501301553729768	24.685796794923938	24.62292225928035
19	26.19410775780254	24.998813688006724	24.26838444643293	24.538694107757802
20	26.280672676214834	24.715580253567005	24.542547295957537	24.461199774260628
21	26.374641351855647	24.48924945302451	24.22012564760034	24.915983547519502
22	26.288334824696978	24.37837251552374	24.10823232734076	25.225060332438513
23	25.581123403563005	24.41345345589631	24.541066731744355	25.464356408796334
24	25.33462585287648	24.6374109839305	24.322698292721782	25.70526487047124
25	25.770468577084145	24.25469822713846	24.512806259670576	25.462026936106824
26	25.428853239151138	24.64469835373232	24.53928570218045	25.387162704936088
27	25.293782687243038	24.8582352362972	24.63842782622843	25.20955425023133
28	25.8600878560679	24.497674796968425	24.289389481676317	25.352847865287366
29	25.349751974795826	24.877597400946684	24.508821969254026	25.26382865500347
30	25.74407939558484	24.703334248497605	24.246938436560477	25.30564791935708
31	25.360471816425452	24.519879334304985	24.560892112666508	25.558756736603055
32	25.591634749008573	24.692404162288582	24.56140053553876	25.154560553164085
33	25.43033540486355	24.564326182119537	24.42790007168726	25.577438341329657
34	25.673325921607443	24.53428149192619	24.43717206502434	25.355220521442035
35	25.515580539705084	24.55991987296272	24.62787871803519	25.29662086929701
36	25.73475329711115	24.58606044788514	24.52030464595246	25.158881609051253
37	25.901571553741782	24.304935319369616	24.449159651760098	25.344333475128504
38	25.742461834648445	24.699693592559452	24.21364462160037	25.344199951191737
39	25.656160973187518	24.715919201574074	24.314096339192048	25.31382348604636
40	25.82245315762358	24.813918490197672	24.11213020960438	25.251498142574363
41	25.44601925589735	24.75608123338073	24.445600649433185	25.35229886128873
42	25.655044776928865	24.54545146397847	24.397324908988605	25.40217885010406
43	25.538099111924616	24.32191037895736	24.310724696630736	25.82926581248729
44	25.46933338304756	24.490584520684227	24.583798698052163	25.45628339821605
45	25.31027084191313	24.4850851369971	24.609481585427652	25.59516243566212
46	25.717186199790188	24.42441220983342	24.342893556297867	25.515508034078525
47	25.618285629064214	24.644654178649933	24.491279685590765	25.24578050669509
48	25.495862095848874	24.814205307160787	24.292031123682914	25.397901473307428
49	25.59453596366348	24.397837434750187	24.33140126093146	25.676225340654874
50	25.28721996159667	24.823702277592012	24.48593600913816	25.403141751673154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	2.5
4	4.0
5	8.5
6	13.0
7	10.5
8	8.0
9	11.0
10	14.0
11	22.0
12	30.0
13	71.5
14	113.0
15	146.5
16	180.0
17	179.0
18	178.0
19	188.5
20	199.0
21	268.5
22	338.0
23	559.5
24	781.0
25	1058.5
26	1336.0
27	1952.5
28	2569.0
29	3428.0
30	4287.0
31	5598.5
32	6910.0
33	8144.5
34	9379.0
35	11219.0
36	13059.0
37	15522.5
38	17986.0
39	20374.5
40	22763.0
41	25892.5
42	29022.0
43	31305.0
44	33588.0
45	37150.5
46	40713.0
47	43071.0
48	45429.0
49	46877.0
50	48325.0
51	49839.5
52	51354.0
53	52071.0
54	52788.0
55	51203.0
56	49618.0
57	46294.5
58	42971.0
59	39867.0
60	36763.0
61	31721.5
62	26680.0
63	23030.5
64	19381.0
65	16498.5
66	13616.0
67	10902.5
68	8189.0
69	6480.5
70	4772.0
71	3779.5
72	2787.0
73	2263.0
74	1739.0
75	1361.5
76	984.0
77	806.0
78	628.0
79	469.5
80	311.0
81	229.5
82	148.0
83	101.5
84	55.0
85	43.5
86	32.0
87	21.5
88	11.0
89	6.0
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	2.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027793595271021446
2	0.004067355405515334
3	6.77892567585889E-4
4	0.0
5	0.0
6	1.6947314189647226E-4
7	0.0
8	0.0
9	0.0
10	3.389462837929445E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.6947314189647226E-4
17	0.0
18	0.0
19	0.0
20	5.084194256894167E-4
21	1.6947314189647226E-4
22	0.0
23	0.0
24	3.389462837929445E-4
25	1.6947314189647226E-4
26	3.389462837929445E-4
27	3.389462837929445E-4
28	0.001355785135171778
29	5.084194256894167E-4
30	0.0010168388513788335
31	6.77892567585889E-4
32	6.77892567585889E-4
33	1.6947314189647226E-4
34	0.001355785135171778
35	1.6947314189647226E-4
36	3.389462837929445E-4
37	0.0018642045608611949
38	0.0
39	5.084194256894167E-4
40	0.0
41	0.0018642045608611949
42	0.004745247973101223
43	0.004067355405515334
44	0.004236828547411807
45	0.002542097128447084
46	0.002542097128447084
47	8.473657094823613E-4
48	0.00525366739879064
49	0.004067355405515334
50	0.0018642045608611949
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	590064.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2053629675558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.99115612891939	35.110834144878005
2	17.20904348408033	18.312268097898897
3	7.138923418533059	11.39487035041907
4	3.5506124878201	7.556465046864187
5	1.94399212208955	5.171540323092177
6	1.2435800224675344	3.969907588475182
7	0.7771553045388541	2.8944181042105765
8	0.5084934872066427	2.164366444277409
9	0.3561075309026098	1.7052147393438102
>10	1.248325845505023	10.335829014504824
>50	0.02685543599212733	0.9540995903637335
>100	0.005754731944761828	0.430186555672187
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6947314189647226E-4
2	0.0	0.0	0.0	0.0	1.6947314189647226E-4
3	0.0	0.0	0.0	0.0	1.6947314189647226E-4
4	0.0	0.0	0.0	0.0	1.6947314189647226E-4
5	0.0	0.0	0.0	0.0	1.6947314189647226E-4
6	0.0	0.0	0.0	0.0	1.6947314189647226E-4
7	0.0	0.0	0.0	0.0	1.6947314189647226E-4
8	0.0	0.0	0.0	0.0	1.6947314189647226E-4
9	0.0	0.0	0.0	0.0	1.6947314189647226E-4
10	0.0	0.0	0.0	5.084194256894167E-4	1.6947314189647226E-4
11	0.0	0.0	0.0	5.084194256894167E-4	1.6947314189647226E-4
12	0.0	0.0	0.0	5.084194256894167E-4	5.084194256894167E-4
13	0.0	0.0	0.0	5.084194256894167E-4	8.473657094823612E-4
14	0.0	0.0	0.0	5.084194256894167E-4	8.473657094823612E-4
15	0.0	0.0	0.0	5.084194256894167E-4	0.0010168388513788335
16	0.0	0.0	0.0	6.77892567585889E-4	0.0010168388513788335
17	0.0	0.0	0.0	6.77892567585889E-4	0.0010168388513788335
18	0.0	0.0	0.0	6.77892567585889E-4	0.0010168388513788335
19	0.0	0.0	0.0	6.77892567585889E-4	0.0010168388513788335
20	0.0	0.0	0.0	8.473657094823612E-4	0.001355785135171778
21	0.0	0.0	0.0	0.0010168388513788335	0.0015252582770682503
22	0.0	0.0	0.0	0.0015252582770682503	0.0015252582770682503
23	0.0	0.0	0.0	0.002033677702757667	0.0015252582770682503
24	0.0	0.0	0.0	0.003389462837929445	0.0015252582770682503
25	0.0	0.0	0.0	0.00457577483120475	0.0015252582770682503
26	0.0	0.0	0.0	0.005423140540687112	0.0015252582770682503
27	0.0	0.0	0.0	0.006439979392065945	0.0015252582770682503
28	0.0	0.0	0.0	0.008643130236720085	0.0016947314189647224
29	0.0	0.0	0.0	0.011015754223270696	0.0016947314189647224
30	0.0	0.0	0.0	0.01287995878413189	0.0016947314189647224
31	0.0	0.0	0.0	0.019150465034301362	0.0016947314189647224
32	0.0	0.0	0.0	0.028132541554814393	0.0016947314189647224
33	0.0	0.0	0.0	0.03677567179153448	0.0016947314189647224
34	0.0	0.0	0.0	0.04474090946066867	0.002542097128447084
35	0.0	0.0	0.0	0.05338403969738876	0.002542097128447084
36	0.0	0.0	0.0	0.06304400878548767	0.002542097128447084
37	0.0	0.0	0.0	0.08151658125220315	0.002542097128447084
38	0.0	0.0	0.0	0.1062596599690881	0.002711570270343556
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	25	0.0023525537	35.19776	6
TGTACCG	45	1.0365347E-6	34.220047	5
GTGTCGC	55	4.957852E-6	27.99822	11
GGTATCA	250	0.0	26.409512	1
GTATAGG	50	8.313925E-5	26.40951	1
GTATCAA	575	0.0	25.644018	1
GCATTAT	45	0.001394437	24.45325	1
TCGTTTA	45	0.0013978845	24.44289	30
GTTATGA	55	1.5883839E-4	24.008648	1
TATACTC	50	0.0025798392	21.998602	3
GTATTAG	75	5.451408E-5	20.540731	1
ACGTGAA	150	4.638423E-10	19.067072	34
TATAAAC	70	8.120063E-4	18.855944	2
GAGCGAT	60	0.007410434	18.332169	11
GTCCTAG	135	5.4886186E-8	17.932384	1
TACGCTC	100	2.4790714E-5	17.600372	34
ACACCGT	75	0.001290358	17.59888	6
CTTAGTT	75	0.001290358	17.59888	14
CCCTAGA	165	1.6461854E-9	17.339577	1
CTACGCT	115	4.2645424E-6	17.217754	33
>>END_MODULE