FastQCFastQC Report
Thu 2 Feb 2017
SRR4062013_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062013_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372925
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7410.19869947040289604No Hit
TATCAACGCAGAGTACTTTTTTTTT4770.12790775625125694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAT250.00602783618.99771717
TACACCG555.8707883E-717.2752915
CTAACAC402.7537486E-416.6252353
TATAAGC456.7445275E-414.7779872
CTTACAC456.7445275E-414.7779873
TAGACTG653.3503347E-614.6175565
GACTGCC400.00526393914.2521167
TAAGCCT602.5575839E-514.2502014
TACACAA551.9513963E-413.8202335
TTACACC551.9536224E-413.8183774
GGTATCA1650.013.8146661
AGACTGC707.2160346E-613.5734436
GAATATG500.001498686613.2966161
ACTGTCC701.0851024E-412.2160998
ACACAAT550.003057511512.0927056
AGACAGT550.003057511512.0927056
TAACACT550.003060590712.091084
CGTCCCC550.003060590712.0910814
GTCTACA550.003066757212.08783151
CCTACAC802.8522514E-511.8751683
Produced by FastQC (version 0.11.5)