##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062013_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372925
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316125226251927	32.0	32.0	32.0	32.0	32.0
2	31.061870349265938	32.0	32.0	32.0	32.0	32.0
3	31.052278608299257	32.0	32.0	32.0	32.0	32.0
4	31.05975464235436	32.0	32.0	32.0	32.0	32.0
5	31.059178118924716	32.0	32.0	32.0	32.0	32.0
6	34.57530870818529	36.0	36.0	36.0	32.0	36.0
7	34.565979754642356	36.0	36.0	36.0	32.0	36.0
8	34.545241000201116	36.0	36.0	36.0	32.0	36.0
9	34.61954816652142	36.0	36.0	36.0	32.0	36.0
10	34.48377555808809	36.0	36.0	36.0	32.0	36.0
11	34.57924783803714	36.0	36.0	36.0	32.0	36.0
12	34.51230944559898	36.0	36.0	36.0	32.0	36.0
13	34.52932090902996	36.0	36.0	36.0	32.0	36.0
14	34.49992894013542	36.0	36.0	36.0	32.0	36.0
15	34.46320037541061	36.0	36.0	36.0	32.0	36.0
16	34.453294898438024	36.0	36.0	36.0	32.0	36.0
17	34.43124220687806	36.0	36.0	36.0	32.0	36.0
18	34.42272574914527	36.0	36.0	36.0	32.0	36.0
19	34.42551183213783	36.0	36.0	36.0	32.0	36.0
20	34.40043440370047	36.0	36.0	36.0	32.0	36.0
21	34.385141784541126	36.0	36.0	36.0	32.0	36.0
22	34.34408259033318	36.0	36.0	36.0	32.0	36.0
23	34.3278568076691	36.0	36.0	36.0	32.0	36.0
24	34.31172755916069	36.0	36.0	36.0	32.0	36.0
25	33.873711872360396	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	3.0
5	26.0
6	63.0
7	23.0
8	64.0
9	53.0
10	25.0
11	2.0
12	15.0
13	18.0
14	64.0
15	101.0
16	155.0
17	201.0
18	282.0
19	327.0
20	468.0
21	561.0
22	733.0
23	1011.0
24	1414.0
25	1934.0
26	2827.0
27	3931.0
28	5406.0
29	7654.0
30	10424.0
31	15297.0
32	23654.0
33	36348.0
34	88588.0
35	171252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.749597768958495	18.58548750402231	12.431888876970932	25.233025850048268
2	15.81501860968991	20.160944321094913	38.95083180487177	25.073205264343407
3	18.60115895238427	24.11167989102308	28.90623533544458	28.380925821148068
4	12.742865877696163	16.35357249169328	36.400449461102575	34.50311216950798
5	14.36840651161044	36.808463269592366	34.10655801465086	14.716572204146336
6	32.93452858488263	35.97158013077647	17.318626122560683	13.775265161780212
7	29.516971104161914	30.933712705847615	21.124964769356723	18.42435142063375
8	26.620748153238278	34.72287837141385	19.93590018897097	18.72047328637691
9	26.651257436161757	15.472585529282906	19.538582136030755	38.33757489852458
10	16.164984548137053	27.948137587361423	32.42072026355427	23.46615760094725
11	35.14040373121104	23.09747439759845	22.608786711991108	19.15333515919941
12	22.994847672055265	24.87669585155711	29.7973167084169	22.331139767970722
13	28.869749185160526	20.755451504298378	25.850413192076594	24.524386118464502
14	22.763012018114416	20.17658064914461	26.40817564647362	30.65223168626735
15	24.036019395058876	28.164718390403422	22.888532107628613	24.91073010690909
16	24.372703087962503	27.308992217679478	24.374045330205014	23.944259364153005
17	22.851275167785236	27.79919463087248	25.838657718120807	23.510872483221476
18	23.053528143815445	27.33717549854644	26.54422561222764	23.065070745410473
19	24.27266285910594	26.36932623352085	26.63154335036716	22.726467557006057
20	24.566771065647906	26.135171184807664	26.3386015904071	22.959456159137325
21	24.74751947783566	26.048367833860702	25.920349540932307	23.28376314737133
22	24.293152964543577	25.864401320557214	26.215207880398317	23.6272378345009
23	24.262379545472843	26.351733927991607	25.746878446589438	23.639008079946116
24	23.418553845658373	26.329971390384376	26.35761482761767	23.893859936339577
25	23.836719358041922	26.264458817530397	26.462252757574944	23.43656906685274
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	16.0
2	66.0
3	116.0
4	116.0
5	116.0
6	221.5
7	327.0
8	327.0
9	327.0
10	441.0
11	555.0
12	555.0
13	555.0
14	885.5
15	1216.0
16	1216.0
17	1216.0
18	2170.0
19	3124.0
20	3124.0
21	3124.0
22	4607.0
23	6090.0
24	6090.0
25	6090.0
26	9050.0
27	12010.0
28	12010.0
29	12010.0
30	15865.5
31	19721.0
32	19721.0
33	19721.0
34	23907.0
35	28093.0
36	28093.0
37	28093.0
38	32694.0
39	37295.0
40	37295.0
41	37295.0
42	41017.0
43	44739.0
44	44739.0
45	44739.0
46	47511.0
47	50283.0
48	50283.0
49	50283.0
50	50270.0
51	50257.0
52	50257.0
53	50257.0
54	46740.5
55	43224.0
56	43224.0
57	43224.0
58	38228.5
59	33233.0
60	33233.0
61	33233.0
62	27689.5
63	22146.0
64	22146.0
65	22146.0
66	16744.5
67	11343.0
68	11343.0
69	11343.0
70	8501.5
71	5660.0
72	5660.0
73	5660.0
74	3923.5
75	2187.0
76	2187.0
77	2187.0
78	1495.5
79	804.0
80	804.0
81	804.0
82	516.5
83	229.0
84	229.0
85	229.0
86	151.5
87	74.0
88	74.0
89	74.0
90	57.5
91	41.0
92	41.0
93	41.0
94	32.5
95	24.0
96	24.0
97	24.0
98	71.0
99	118.0
100	118.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013407521619628613
2	2.6815043239257225E-4
3	5.363008647851445E-4
4	0.009117114701347455
5	0.030569149292753235
6	0.06113829858550647
7	0.10189716430917745
8	0.10216531474157003
9	0.1128913320372729
10	0.12817590668364953
11	0.13380706576389356
12	0.12656700408929408
13	0.12308104846819065
14	0.10833277468659919
15	0.12308104846819065
16	0.1110142790105249
17	0.1139639337668432
18	0.10511496949788832
19	0.08929409398672655
20	0.08473553663605282
21	0.08634443923040826
22	0.09519340349936314
23	0.07347321847556479
24	0.08661258966280083
25	0.08446738620366026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	372925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.275964569998706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.81355947689801	27.368549693446443
2	15.89352141954952	14.074019825289803
3	7.727600193762589	10.264408571704427
4	4.4486824512278655	7.878788263749599
5	2.866515860368698	6.3458877336511925
6	1.766255212096146	4.692159191540671
7	1.2663408470999238	3.924792373581686
8	0.9351931275729005	3.3125262226019214
9	0.6661748179530406	2.654597937340274
>10	2.5730763832974652	17.52876390568343
>50	0.03215846674945848	0.9456494175800483
>100	0.010314979900769686	0.8107869154138019
>500	6.067635235746874E-4	0.19906994841670883
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	741	0.19869947040289604	No Hit
TATCAACGCAGAGTACTTTTTTTTT	477	0.12790775625125694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAT	25	0.006027836	18.997717	17
TACACCG	55	5.8707883E-7	17.275291	5
CTAACAC	40	2.7537486E-4	16.625235	3
TATAAGC	45	6.7445275E-4	14.777987	2
CTTACAC	45	6.7445275E-4	14.777987	3
TAGACTG	65	3.3503347E-6	14.617556	5
GACTGCC	40	0.005263939	14.252116	7
TAAGCCT	60	2.5575839E-5	14.250201	4
TACACAA	55	1.9513963E-4	13.820233	5
TTACACC	55	1.9536224E-4	13.818377	4
GGTATCA	165	0.0	13.814666	1
AGACTGC	70	7.2160346E-6	13.573443	6
GAATATG	50	0.0014986866	13.296616	1
ACTGTCC	70	1.0851024E-4	12.216099	8
ACACAAT	55	0.0030575115	12.092705	6
AGACAGT	55	0.0030575115	12.092705	6
TAACACT	55	0.0030605907	12.09108	4
CGTCCCC	55	0.0030605907	12.09108	14
GTCTACA	55	0.0030667572	12.0878315	1
CCTACAC	80	2.8522514E-5	11.875168	3
>>END_MODULE