FastQCFastQC Report
Thu 2 Feb 2017
SRR4062013_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062013_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372925
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTT300.005740670529.33261725
GGTATCA2050.025.7589231
GGCGTGC450.00139660224.4438488
GTGTAGG803.6010224E-622.0024151
TAGTAGT500.002575482222.0024151
TCTAGGC602.8680955E-421.9994643
TTAGGTA500.002577488821.9994624
ACTATAC500.002577488821.9994623
GTATCAA3600.020.7800561
GCATAAA755.4550277E-520.5355851
TCTTAAG755.4606648E-520.5328332
GAATAGA654.919729E-420.309921
ACAGTAA550.004475056720.00219513
TATAGTA1051.7863367E-618.8566842
GCCTATC708.1102736E-418.85668241
AGTGCAC951.590783E-518.52834910
GTATAGT951.590783E-518.5283491
GTGTATA600.007398052618.3353441
CTATACA600.007403778418.3328864
TAGCCTA600.007403778418.3328865
Produced by FastQC (version 0.11.5)