FastQCFastQC Report
Thu 2 Feb 2017
SRR4062012_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062012_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746272
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT11540.1546353072338236No Hit
TATCAACGCAGAGTACTTTTTTTTT8280.11095150293726685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT551.1280952E-515.5434772
GGTATCA2850.013.9907091
TAAATCC551.9567655E-413.8192064
AGGCCCG1450.013.76148910
CGACCCG500.001495917213.3027735
GTTTTCG500.001499705813.29830915
GCGTTAT655.479087E-513.1451171
GTTATTC905.3918484E-712.6667573
TCCAACG604.096608E-412.66335718
GCCGCCT1551.8189894E-1212.25486118
CGCTATT701.0873124E-412.21683211
GTAATAC701.0893588E-412.2143723
GTATAGG701.09620465E-412.206181
GTATCAA7800.012.1714031
ACGGTAT1251.3988029E-912.1633529
AGGACTA550.003061085712.093435
CGAGCCG1501.4551915E-1112.03180315
TCTTATA951.0389449E-611.9984742
GGACTTT657.9963094E-411.695536
GGTTGCA1406.730261E-1011.5388947
Produced by FastQC (version 0.11.5)