##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062012_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746272
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315715985592384	32.0	32.0	32.0	32.0	32.0
2	30.93579016766005	32.0	32.0	32.0	32.0	32.0
3	30.969358357274558	32.0	32.0	32.0	32.0	32.0
4	30.978022758458042	32.0	32.0	32.0	32.0	32.0
5	30.91819202649972	32.0	32.0	32.0	32.0	32.0
6	34.546185573088636	36.0	36.0	36.0	32.0	36.0
7	34.493621628575106	36.0	36.0	36.0	32.0	36.0
8	34.462524387890745	36.0	36.0	36.0	32.0	36.0
9	34.58255703014451	36.0	36.0	36.0	32.0	36.0
10	34.34469871574975	36.0	36.0	36.0	32.0	36.0
11	34.54346940525706	36.0	36.0	36.0	32.0	36.0
12	34.415063140517134	36.0	36.0	36.0	32.0	36.0
13	34.47699096308049	36.0	36.0	36.0	32.0	36.0
14	34.41142907679774	36.0	36.0	36.0	32.0	36.0
15	34.35830903477552	36.0	36.0	36.0	32.0	36.0
16	34.369010226834185	36.0	36.0	36.0	32.0	36.0
17	34.32724395394709	36.0	36.0	36.0	32.0	36.0
18	34.32068200334462	36.0	36.0	36.0	32.0	36.0
19	34.31006389091377	36.0	36.0	36.0	32.0	36.0
20	34.29387274345011	36.0	36.0	36.0	32.0	36.0
21	34.2779978345697	36.0	36.0	36.0	32.0	36.0
22	34.25100097551563	36.0	36.0	36.0	32.0	36.0
23	34.21771016465846	36.0	36.0	36.0	32.0	36.0
24	34.190989344367736	36.0	36.0	36.0	32.0	36.0
25	33.83805368551949	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	10.0
5	53.0
6	153.0
7	32.0
8	129.0
9	107.0
10	67.0
11	19.0
12	26.0
13	25.0
14	115.0
15	210.0
16	291.0
17	381.0
18	465.0
19	625.0
20	897.0
21	1171.0
22	1688.0
23	2483.0
24	3403.0
25	4942.0
26	6871.0
27	9060.0
28	12526.0
29	17028.0
30	23034.0
31	32014.0
32	47422.0
33	69285.0
34	159479.0
35	352261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.981802686448766	17.54834750168841	11.655392729650634	25.814457082212193
2	16.575479952992946	19.485012817059754	37.977222751520564	25.96228447842674
3	19.283503289694078	22.964610663700803	28.17371728730888	29.57816875929623
4	12.862602887689928	15.590550547974946	35.293660429749586	36.25318613458554
5	14.866326646873135	36.4863578786518	33.36974806812013	15.27756740635493
6	34.83281199511421	34.72528179749731	16.565281690235263	13.87662451715321
7	30.71421385503786	30.046612698774656	20.11173633979651	19.12743710639097
8	27.936169813193683	33.78288829449025	18.983122238349086	19.297819653966986
9	26.879327299094598	14.624778478507888	18.612769028712943	39.88312519368458
10	16.527062763392518	27.124347430577078	31.073910237574847	25.274679568455554
11	36.9917586472481	21.301387692448063	21.979400643528617	19.72745301677522
12	24.331921390993536	23.617855144644704	28.97549875286626	23.074724711495502
13	29.65164250592	19.675054168818885	24.88196899464014	25.79133433062098
14	23.148405272160176	19.766844417614113	25.13800851863031	31.9467417915954
15	24.948445032408856	27.562486499105752	21.986499776606323	25.50256869187907
16	25.328346315142703	25.8498099012345	24.06701350412261	24.75483027950019
17	23.84590202644685	26.317244118152157	25.19896852996737	24.637885325433626
18	24.377620914190967	25.538134217636156	26.15682423676564	23.927420631407237
19	25.47900557302949	25.040540268658916	25.124906948514862	24.35554720979673
20	25.579979264659926	24.84391492877424	24.904672425887252	24.67143338067858
21	25.9456218800044	24.530910924712376	24.707548612247415	24.815918583035806
22	25.47098567291428	24.6821307800958	25.10868384235779	24.738199704632123
23	24.393697999645955	24.79521717439933	25.476753729541834	25.334331096412882
24	24.628871565687184	25.017302337559215	25.539054997290645	24.814771099462956
25	24.746984332207628	24.836710005016055	25.27796181897678	25.13834384379954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	300.0
1	300.0
2	268.0
3	236.0
4	236.0
5	236.0
6	506.5
7	777.0
8	777.0
9	777.0
10	971.0
11	1165.0
12	1165.0
13	1165.0
14	1647.0
15	2129.0
16	2129.0
17	2129.0
18	3463.5
19	4798.0
20	4798.0
21	4798.0
22	7713.5
23	10629.0
24	10629.0
25	10629.0
26	15636.0
27	20643.0
28	20643.0
29	20643.0
30	26698.5
31	32754.0
32	32754.0
33	32754.0
34	39739.0
35	46724.0
36	46724.0
37	46724.0
38	54320.5
39	61917.0
40	61917.0
41	61917.0
42	70200.5
43	78484.0
44	78484.0
45	78484.0
46	86689.5
47	94895.0
48	94895.0
49	94895.0
50	97739.5
51	100584.0
52	100584.0
53	100584.0
54	95336.0
55	90088.0
56	90088.0
57	90088.0
58	83008.0
59	75928.0
60	75928.0
61	75928.0
62	65754.0
63	55580.0
64	55580.0
65	55580.0
66	44914.0
67	34248.0
68	34248.0
69	34248.0
70	25941.0
71	17634.0
72	17634.0
73	17634.0
74	13159.0
75	8684.0
76	8684.0
77	8684.0
78	6766.5
79	4849.0
80	4849.0
81	4849.0
82	3273.0
83	1697.0
84	1697.0
85	1697.0
86	1277.0
87	857.0
88	857.0
89	857.0
90	561.0
91	265.0
92	265.0
93	265.0
94	168.0
95	71.0
96	71.0
97	71.0
98	203.5
99	336.0
100	336.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010719951974615153
2	1.339993996826894E-4
3	2.679987993653788E-4
4	0.00937995797778826
5	0.03175785772479739
6	0.0582897388619699
7	0.10277753955662279
8	0.1063955233480554
9	0.1151054843274302
10	0.12716543029887226
11	0.13480339608078556
12	0.1290414218944299
13	0.1228774495090262
14	0.11349749153123795
15	0.12743342909823765
16	0.1165794777239398
17	0.12435144290553579
18	0.11068350413790146
19	0.09607756957248832
20	0.09178958878264226
21	0.09085159298486344
22	0.10197354315852666
23	0.08093563740834442
24	0.09487157497534411
25	0.08924360018867115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	746272.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.43087926263007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46818764497417	50.40737015728387
2	13.225613873199176	16.77824633530124
3	3.5205497728653548	6.699347027421061
4	1.3992216089183571	3.550154277478532
5	0.6771596232380667	2.1476415151570936
6	0.41891902611512444	1.5943441299796217
7	0.2498518772570333	1.1093826981882642
8	0.1778786438629259	0.9026399025815701
9	0.12974865332378568	0.7407064047122907
>10	0.6253952474698224	7.149023653571588
>50	0.06053429385751649	2.6844413006589187
>100	0.04524055899499156	5.52629620373375
>500	0.0014867789332205926	0.5549334224553522
>1k	2.1239699046008467E-4	0.15547297147693043
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1154	0.1546353072338236	No Hit
TATCAACGCAGAGTACTTTTTTTTT	828	0.11095150293726685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	55	1.1280952E-5	15.543477	2
GGTATCA	285	0.0	13.990709	1
TAAATCC	55	1.9567655E-4	13.819206	4
AGGCCCG	145	0.0	13.761489	10
CGACCCG	50	0.0014959172	13.302773	5
GTTTTCG	50	0.0014997058	13.298309	15
GCGTTAT	65	5.479087E-5	13.145117	1
GTTATTC	90	5.3918484E-7	12.666757	3
TCCAACG	60	4.096608E-4	12.663357	18
GCCGCCT	155	1.8189894E-12	12.254861	18
CGCTATT	70	1.0873124E-4	12.216832	11
GTAATAC	70	1.0893588E-4	12.214372	3
GTATAGG	70	1.09620465E-4	12.20618	1
GTATCAA	780	0.0	12.171403	1
ACGGTAT	125	1.3988029E-9	12.163352	9
AGGACTA	55	0.0030610857	12.09343	5
CGAGCCG	150	1.4551915E-11	12.031803	15
TCTTATA	95	1.0389449E-6	11.998474	2
GGACTTT	65	7.9963094E-4	11.69553	6
GGTTGCA	140	6.730261E-10	11.538894	7
>>END_MODULE