FastQCFastQC Report
Thu 2 Feb 2017
SRR4062012_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062012_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746272
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG207.854233E-444.00053835
TAACGGC300.005741429.33565736
CGTCGTA1500.027.86513910
GTATCAA8400.027.2439061
TAGGACG1550.026.9662654
ATACCGT1750.026.3985546
GGTATCA3500.023.8908081
CCGTCGT1750.023.8844059
GTAGGAC1950.023.691013
ACCGTCG1800.023.220958
GTCGTAG2050.022.53534911
CGCGTAT500.00257733222.00321642
ACGCGTA602.8685134E-422.00321641
GCATACG500.00257833522.00174137
TACCGTC2000.021.9987937
CGTGATT1351.1277734E-1021.18544428
GTCCTAA1700.020.7102991
TACGCGT654.924873E-420.31066140
ATACGCG550.00447824120.00292439
TCGTACT550.004481715620.00024444
Produced by FastQC (version 0.11.5)