##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746272
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262822402555635	32.0	32.0	32.0	32.0	32.0
2	31.39352943698812	32.0	32.0	32.0	32.0	32.0
3	31.547202628532226	32.0	32.0	32.0	32.0	32.0
4	31.62984809828052	32.0	32.0	32.0	32.0	32.0
5	31.595743911067277	32.0	32.0	32.0	32.0	32.0
6	35.24705067321298	36.0	36.0	36.0	36.0	36.0
7	35.2523222095965	36.0	36.0	36.0	36.0	36.0
8	35.201163918785646	36.0	36.0	36.0	36.0	36.0
9	35.30137670983234	36.0	36.0	36.0	36.0	36.0
10	35.162459532181295	36.0	36.0	36.0	36.0	36.0
11	35.302329445564084	36.0	36.0	36.0	36.0	36.0
12	35.2214527678916	36.0	36.0	36.0	36.0	36.0
13	35.25785102482741	36.0	36.0	36.0	36.0	36.0
14	35.219390517130485	36.0	36.0	36.0	36.0	36.0
15	35.18686216285751	36.0	36.0	36.0	36.0	36.0
16	35.206238476051624	36.0	36.0	36.0	36.0	36.0
17	35.18777871875134	36.0	36.0	36.0	36.0	36.0
18	35.180763850177954	36.0	36.0	36.0	36.0	36.0
19	35.17420457956349	36.0	36.0	36.0	36.0	36.0
20	35.16065590026157	36.0	36.0	36.0	36.0	36.0
21	35.15957050512414	36.0	36.0	36.0	36.0	36.0
22	35.13537155353544	36.0	36.0	36.0	36.0	36.0
23	35.113305872389695	36.0	36.0	36.0	36.0	36.0
24	35.10045934994211	36.0	36.0	36.0	36.0	36.0
25	35.07720375412718	36.0	36.0	36.0	36.0	36.0
26	35.03838278804511	36.0	36.0	36.0	36.0	36.0
27	35.028430652630675	36.0	36.0	36.0	36.0	36.0
28	35.00382166287895	36.0	36.0	36.0	36.0	36.0
29	34.98941136743707	36.0	36.0	36.0	36.0	36.0
30	34.966108871832255	36.0	36.0	36.0	36.0	36.0
31	34.971128489344366	36.0	36.0	36.0	36.0	36.0
32	34.936299365378844	36.0	36.0	36.0	36.0	36.0
33	34.91800710732816	36.0	36.0	36.0	32.0	36.0
34	34.903930470391494	36.0	36.0	36.0	32.0	36.0
35	34.894333433386215	36.0	36.0	36.0	32.0	36.0
36	34.86812181081429	36.0	36.0	36.0	32.0	36.0
37	34.8495776338922	36.0	36.0	36.0	32.0	36.0
38	34.831340315595384	36.0	36.0	36.0	32.0	36.0
39	34.796268652716435	36.0	36.0	36.0	32.0	36.0
40	34.79635843231422	36.0	36.0	36.0	32.0	36.0
41	34.76653284593285	36.0	36.0	36.0	32.0	36.0
42	34.74478742335234	36.0	36.0	36.0	32.0	36.0
43	34.74237007418207	36.0	36.0	36.0	32.0	36.0
44	34.69586692251619	36.0	36.0	36.0	32.0	36.0
45	34.672505467175505	36.0	36.0	36.0	32.0	36.0
46	34.64812829638523	36.0	36.0	36.0	32.0	36.0
47	34.62139407615454	36.0	36.0	36.0	32.0	36.0
48	34.59715626473994	36.0	36.0	36.0	32.0	36.0
49	34.58489129968698	36.0	36.0	36.0	32.0	36.0
50	34.16354760730672	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	5.0
21	11.0
22	30.0
23	123.0
24	286.0
25	746.0
26	1604.0
27	3055.0
28	5457.0
29	8916.0
30	13898.0
31	21100.0
32	32392.0
33	56756.0
34	130513.0
35	471377.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.737500737221936	18.04357383746803	12.04446386540204	26.174461559907996
2	16.042131500130655	19.944923516050576	37.46969788337454	26.54324710044423
3	18.66203741546279	23.42680722366868	28.47686290064307	29.43429246022547
4	12.658253291025257	16.04307812700999	35.3333637065306	35.96530487543416
5	14.589070190319603	36.78757448701611	33.25708757274502	15.366267749919265
6	34.98313615295248	34.89389243317776	16.385870548366476	13.737100865503283
7	30.69082050512414	30.023905492903392	20.193709532181295	19.091564469791177
8	27.902962994725783	33.80657454654603	18.965471034689767	19.32499142403842
9	27.03571887997942	14.432405342824065	18.523541014536253	40.00833476266026
10	16.39513848875072	27.09207123427178	31.294705669529794	25.218084607447704
11	37.35943462973286	21.08373354487372	21.930609750868317	19.626222074525106
12	24.319283049611936	23.559908451610138	28.868964667038295	23.25184383173963
13	29.730580806997985	19.56619034346726	25.009915955576517	25.693312893958236
14	23.25264782813773	19.547966425110417	25.16307726941383	32.03630847733802
15	25.03979782170576	27.566490502122548	21.93221774366451	25.461493932507185
16	25.52377225906476	25.700384177823278	24.05727693365261	24.718566629459353
17	24.00197247116333	26.203984606148968	25.23731293683804	24.556729985849664
18	24.42356730999865	25.38916827801161	26.27195750605343	23.915306905936315
19	25.68312893958235	24.923620342180865	25.144183354058576	24.24906736417821
20	25.622801551727605	24.77886541644054	25.00961454711128	24.588718484720577
21	26.22864049569058	24.394188714034563	24.64892157283135	24.72824921744351
22	25.775856524162773	24.60697976073067	25.019295913554306	24.597867801552248
23	24.63337764246816	24.513046181553104	25.430004073581753	25.423572102396978
24	24.936483603657685	24.903251396288713	25.335404092921536	24.82486090713206
25	24.983651985613747	24.646105688573005	25.394764347392623	24.97547797842062
26	24.499878730055784	25.503672268310755	25.691808181768238	24.304640819865224
27	25.032360941750305	24.972597049325312	25.09547482814531	24.899567180779076
28	24.584625898653943	25.177184742480783	25.259060240802405	24.979129118062858
29	24.694044640739467	24.90442454396471	25.38896832107457	25.012562494221253
30	24.665431170679348	25.204922151692642	25.31868962384373	24.81095705378428
31	25.21560503408945	24.77890099052356	24.94867822992153	25.05681574546546
32	24.840573146072384	25.180129899888374	24.887741250785577	25.09155570325366
33	24.488991919814545	24.819033325740016	25.446152196926047	25.2458225575194
34	25.029714486331333	24.958962518877243	25.509434265660236	24.501888729131185
35	25.18840315595386	24.872566570901764	25.528359632948845	24.41067064019553
36	24.41254663179109	25.37653831310836	25.216945028086275	24.993970027014278
37	25.303847710321577	24.927036349164233	24.97688479381236	24.79223114670183
38	24.756255091977188	24.932598301959608	25.232354959049786	25.07879164701342
39	25.379220333713892	24.683893721826475	24.929515937974024	25.00737000648561
40	25.474089876077354	24.928980318168176	25.222573002872945	24.374356802881522
41	24.681749719939752	25.197114173460474	25.51697245493576	24.604163651664013
42	25.584499294460073	25.421682387766893	24.974907435573417	24.018910882199624
43	24.928910460681657	24.77507296560633	25.384526735294983	24.91148983841703
44	24.648912163719494	25.198592432583094	24.970921350964552	25.18157405273286
45	24.67712238930772	25.392022598082704	25.08140616248	24.84944885012958
46	24.733571766446325	25.14401797762445	24.89343540664727	25.228974849281954
47	25.106229020522203	25.05664877757901	25.187567419080352	24.649554782818438
48	25.308574973902953	25.329479412478072	24.592329947082288	24.769615666536684
49	24.951959026476807	25.404227605670044	24.657146915595295	24.986666452257857
50	24.841978878703294	25.702937712140926	24.698999411733965	24.75608399742181
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	88.5
2	38.0
3	79.5
4	121.0
5	122.0
6	123.0
7	87.5
8	52.0
9	90.5
10	129.0
11	214.0
12	299.0
13	578.5
14	858.0
15	1032.0
16	1206.0
17	1254.0
18	1302.0
19	1332.0
20	1362.0
21	1650.0
22	1938.0
23	2397.0
24	2856.0
25	3688.5
26	4521.0
27	5939.5
28	7358.0
29	8725.5
30	10093.0
31	11534.0
32	12975.0
33	15064.5
34	17154.0
35	19862.5
36	22571.0
37	25419.0
38	28267.0
39	30430.0
40	32593.0
41	35108.0
42	37623.0
43	38968.0
44	40313.0
45	44160.5
46	48008.0
47	50686.0
48	53364.0
49	55650.0
50	57936.0
51	57158.5
52	56381.0
53	55815.5
54	55250.0
55	54960.0
56	54670.0
57	52688.0
58	50706.0
59	46815.5
60	42925.0
61	37600.0
62	32275.0
63	27716.5
64	23158.0
65	19529.5
66	15901.0
67	13404.0
68	10907.0
69	9563.5
70	8220.0
71	6440.5
72	4661.0
73	3968.5
74	3276.0
75	2410.5
76	1545.0
77	1400.0
78	1255.0
79	1024.0
80	793.0
81	648.5
82	504.0
83	419.0
84	334.0
85	266.0
86	198.0
87	135.5
88	73.0
89	48.0
90	23.0
91	15.0
92	7.0
93	4.0
94	1.0
95	4.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03055186312765319
2	0.004957977788259509
3	4.0199819904806827E-4
4	0.0
5	1.339993996826894E-4
6	1.339993996826894E-4
7	0.0
8	0.0
9	0.0
10	2.679987993653788E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.0199819904806827E-4
17	0.0
18	1.339993996826894E-4
19	0.0
20	9.37995797778826E-4
21	0.0
22	0.0
23	0.0
24	0.0010719951974615153
25	5.359975987307577E-4
26	4.0199819904806827E-4
27	2.679987993653788E-4
28	0.00227798979460572
29	4.0199819904806827E-4
30	0.0017419921958749624
31	0.0
32	6.699969984134471E-4
33	2.679987993653788E-4
34	4.0199819904806827E-4
35	0.0
36	0.0
37	0.0013399939968268943
38	0.0
39	5.359975987307577E-4
40	0.0
41	5.359975987307577E-4
42	0.00468997898889413
43	0.0040199819904806824
44	0.0026799879936537886
45	0.001875991595557652
46	0.0014739933965095837
47	9.37995797778826E-4
48	0.002545988593971099
49	0.00455597958921144
50	0.0014739933965095837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	746272.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.83970631548428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.09635678904606	52.73520607931038
2	12.899712671322238	16.986265876679752
3	3.480127747039448	6.873917664163357
4	1.3426719377491483	3.5360450423778444
5	0.6508800364383398	2.1426875222856
6	0.39616209712367995	1.5649917676769671
7	0.22410647714039755	1.0328573246825097
8	0.16084877046746182	0.8472188647027543
9	0.11142446118692843	0.66025384208176
>10	0.5512110869992692	6.454468857574515
>50	0.04785041130797355	2.2197149017234774
>100	0.037829577372486996	4.649778406143108
>500	8.179368065533466E-4	0.29659385059799653
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.339993996826894E-4
2	0.0	0.0	0.0	0.0	1.339993996826894E-4
3	0.0	0.0	0.0	0.0	1.339993996826894E-4
4	0.0	0.0	0.0	0.0	1.339993996826894E-4
5	0.0	0.0	0.0	0.0	1.339993996826894E-4
6	0.0	0.0	0.0	0.0	1.339993996826894E-4
7	0.0	0.0	0.0	0.0	1.339993996826894E-4
8	0.0	0.0	0.0	0.0	1.339993996826894E-4
9	0.0	0.0	0.0	0.0	1.339993996826894E-4
10	0.0	0.0	0.0	0.0	1.339993996826894E-4
11	0.0	0.0	0.0	0.0	1.339993996826894E-4
12	0.0	0.0	0.0	0.0	4.0199819904806827E-4
13	0.0	0.0	0.0	0.0	4.0199819904806827E-4
14	0.0	0.0	0.0	0.0	4.0199819904806827E-4
15	0.0	0.0	0.0	0.0	5.359975987307577E-4
16	0.0	0.0	0.0	0.0	5.359975987307577E-4
17	0.0	0.0	0.0	0.0	5.359975987307577E-4
18	0.0	0.0	0.0	0.0	5.359975987307577E-4
19	0.0	0.0	0.0	0.0	6.699969984134471E-4
20	0.0	0.0	0.0	1.339993996826894E-4	8.039963980961365E-4
21	0.0	0.0	0.0	1.339993996826894E-4	0.0010719951974615153
22	0.0	0.0	0.0	1.339993996826894E-4	0.0010719951974615153
23	0.0	0.0	0.0	4.0199819904806827E-4	0.0010719951974615153
24	0.0	0.0	0.0	6.699969984134471E-4	0.0010719951974615153
25	0.0	0.0	0.0	0.0012059945971442047	0.0010719951974615153
26	0.0	0.0	0.0	0.0020099909952403412	0.0010719951974615153
27	0.0	0.0	0.0	0.0021439903949230306	0.0010719951974615153
28	0.0	0.0	0.0	0.002813987393336478	0.0010719951974615153
29	0.0	0.0	0.0	0.0037519831911153037	0.0010719951974615153
30	0.0	0.0	0.0	0.005091977187942198	0.0010719951974615153
31	0.0	0.0	0.0	0.008173963380644056	0.0010719951974615153
32	0.0	0.0	0.0	0.013399939968268942	0.0010719951974615153
33	0.0	0.0	0.0	0.017285922559066934	0.0010719951974615153
34	0.0	0.0	0.0	0.022109900947643753	0.0010719951974615153
35	0.0	0.0	0.0	0.028005874533682088	0.0010719951974615153
36	0.0	0.0	0.0	0.039529822906393376	0.0010719951974615153
37	0.0	0.0	0.0	0.051991767076883494	0.0010719951974615153
38	0.0	0.0	0.0	0.06914369023626774	0.0010719951974615153
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	20	7.854233E-4	44.000538	35
TAACGGC	30	0.0057414	29.335657	36
CGTCGTA	150	0.0	27.865139	10
GTATCAA	840	0.0	27.243906	1
TAGGACG	155	0.0	26.966265	4
ATACCGT	175	0.0	26.398554	6
GGTATCA	350	0.0	23.890808	1
CCGTCGT	175	0.0	23.884405	9
GTAGGAC	195	0.0	23.69101	3
ACCGTCG	180	0.0	23.22095	8
GTCGTAG	205	0.0	22.535349	11
CGCGTAT	50	0.002577332	22.003216	42
ACGCGTA	60	2.8685134E-4	22.003216	41
GCATACG	50	0.002578335	22.001741	37
TACCGTC	200	0.0	21.998793	7
CGTGATT	135	1.1277734E-10	21.185444	28
GTCCTAA	170	0.0	20.710299	1
TACGCGT	65	4.924873E-4	20.310661	40
ATACGCG	55	0.004478241	20.002924	39
TCGTACT	55	0.0044817156	20.000244	44
>>END_MODULE