FastQCFastQC Report
Thu 2 Feb 2017
SRR4062011_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062011_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076695
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20250.18807554599956347No Hit
TATCAACGCAGAGTACTTTTTTTTT13520.12556945095872088No Hit
GCGCAAGACGGACCAGAGCGAAAGC11310.10504367532123766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5650.013.2785371
CGCAAGA2100.013.1150672
AATCGAA1102.73576E-912.9566387
AGACCCG751.4765381E-512.6675355
TAGGACG751.4765381E-512.6675354
CGACCAT2500.012.542026510
GATAATC701.0886539E-412.2162597
GTAAGAC701.0900732E-412.2145553
ATCCCGA550.003067951212.09061316
CATCCCG550.003067951212.09061315
AAGACGG2300.011.9790815
CCGTCAA1353.7107384E-1011.96155618
GCGCAAG2400.011.8708441
CGACCCG1052.7142414E-711.7627115
GGTCGGC907.4449235E-611.61244711
TTAGGAC907.4526397E-611.6113663
CCCGAAA907.464225E-611.60974618
ATCGAAC1157.0476744E-811.5670868
CCGACCA2500.011.4018429
TCCGTCA1501.7644197E-1011.39866117
Produced by FastQC (version 0.11.5)