##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062011_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1076695
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.284135247214856	32.0	32.0	32.0	32.0	32.0
2	30.86860995918064	32.0	32.0	32.0	32.0	32.0
3	30.89107500267021	32.0	32.0	32.0	32.0	32.0
4	30.896953176154806	32.0	32.0	32.0	32.0	32.0
5	30.838843869433777	32.0	32.0	32.0	32.0	32.0
6	34.486275128982676	36.0	36.0	36.0	32.0	36.0
7	34.42641323680336	36.0	36.0	36.0	32.0	36.0
8	34.39393050028095	36.0	36.0	36.0	32.0	36.0
9	34.50192672948235	36.0	36.0	36.0	32.0	36.0
10	34.23444615234584	36.0	36.0	36.0	32.0	36.0
11	34.477635727852366	36.0	36.0	36.0	32.0	36.0
12	34.32227511040731	36.0	36.0	36.0	32.0	36.0
13	34.40089997631641	36.0	36.0	36.0	32.0	36.0
14	34.30860921616614	36.0	36.0	36.0	32.0	36.0
15	34.27626672363111	36.0	36.0	36.0	32.0	36.0
16	34.29041557729905	36.0	36.0	36.0	32.0	36.0
17	34.216128987317674	36.0	36.0	36.0	32.0	36.0
18	34.21944004569539	36.0	36.0	36.0	32.0	36.0
19	34.22769122174803	36.0	36.0	36.0	32.0	36.0
20	34.2051258713006	36.0	36.0	36.0	32.0	36.0
21	34.18866902883361	36.0	36.0	36.0	32.0	36.0
22	34.14822953575525	36.0	36.0	36.0	32.0	36.0
23	34.13815797417096	36.0	36.0	36.0	32.0	36.0
24	34.103114623918565	36.0	36.0	36.0	32.0	36.0
25	33.75608041274456	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	15.0
5	84.0
6	203.0
7	40.0
8	193.0
9	150.0
10	93.0
11	14.0
12	34.0
13	54.0
14	189.0
15	272.0
16	408.0
17	532.0
18	709.0
19	942.0
20	1430.0
21	2078.0
22	3049.0
23	4334.0
24	6074.0
25	8504.0
26	11600.0
27	14932.0
28	19909.0
29	26264.0
30	34436.0
31	46925.0
32	66723.0
33	96187.0
34	216623.0
35	513695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44362804512765	17.3026500934819	11.504264502233257	25.749457359157184
2	16.793954811547604	19.284827308856485	37.547727249507986	26.373490630087925
3	19.80594174537261	22.458987616642535	27.776689047048958	29.9583815909359
4	12.98534344309728	15.38645882389003	34.726816232468835	36.90138150054385
5	15.281932743441761	35.926496437981484	33.04120569774312	15.750365120833628
6	35.19370580434352	34.30368230147792	16.428958429945382	14.073653464233175
7	31.132439475883793	29.740424317311025	19.777735009478757	19.349401197326426
8	28.07353233775338	33.194056272395436	18.965203048847386	19.7672083410038
9	26.808347226471007	14.86455701734403	18.66103316593381	39.66606259025116
10	16.930067497819202	26.567896779857413	30.39522214389739	26.106813578425992
11	37.00166476009784	21.418952224175293	21.747812096016666	19.831570919710202
12	24.59669657130475	23.519236831900102	28.314983534934672	23.569083061860475
13	29.595636098526274	19.389641499468087	24.89147900997612	26.123243392029522
14	23.564044534284964	19.549115469657437	24.83426097596807	32.052579020089524
15	25.248696418790168	27.125183784723806	21.848551157948382	25.777568638537645
16	25.85193671008488	25.82292511288487	23.384928103311044	24.940210073719214
17	24.081196541456585	26.074657372466675	24.93588229495818	24.90826379111856
18	24.81989973147673	25.28442267496783	25.71630999933055	24.17936759422489
19	25.635824258983632	25.026425857457486	25.049016827948844	24.288733055610045
20	25.68907977057013	24.591758001691904	24.76885034070521	24.95031188703275
21	26.31928279800992	24.325299144027905	24.55472346374947	24.800694594212707
22	25.792314164454755	24.411057859921996	24.88985371159755	24.906774264025696
23	24.538796665263096	24.461181955834906	25.300443286885343	25.69957809201665
24	24.608734452562885	24.91345492405228	25.30890363842564	25.16890698495919
25	24.92135616917162	24.616262726170735	25.132653364766078	25.329727739891574
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	664.0
1	664.0
2	519.0
3	374.0
4	374.0
5	374.0
6	817.0
7	1260.0
8	1260.0
9	1260.0
10	1450.0
11	1640.0
12	1640.0
13	1640.0
14	2160.0
15	2680.0
16	2680.0
17	2680.0
18	4484.5
19	6289.0
20	6289.0
21	6289.0
22	10073.0
23	13857.0
24	13857.0
25	13857.0
26	20405.0
27	26953.0
28	26953.0
29	26953.0
30	34306.0
31	41659.0
32	41659.0
33	41659.0
34	52111.0
35	62563.0
36	62563.0
37	62563.0
38	73919.0
39	85275.0
40	85275.0
41	85275.0
42	99364.5
43	113454.0
44	113454.0
45	113454.0
46	126008.5
47	138563.0
48	138563.0
49	138563.0
50	143823.0
51	149083.0
52	149083.0
53	149083.0
54	140331.0
55	131579.0
56	131579.0
57	131579.0
58	121272.0
59	110965.0
60	110965.0
61	110965.0
62	97556.5
63	84148.0
64	84148.0
65	84148.0
66	68309.5
67	52471.0
68	52471.0
69	52471.0
70	39705.0
71	26939.0
72	26939.0
73	26939.0
74	19975.5
75	13012.0
76	13012.0
77	13012.0
78	10378.5
79	7745.0
80	7745.0
81	7745.0
82	5379.0
83	3013.0
84	3013.0
85	3013.0
86	2249.5
87	1486.0
88	1486.0
89	1486.0
90	993.5
91	501.0
92	501.0
93	501.0
94	300.5
95	100.0
96	100.0
97	100.0
98	261.0
99	422.0
100	422.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0014860290054286498
2	3.7150725135716244E-4
3	7.430145027143249E-4
4	0.010402203038000548
5	0.03148523955251952
6	0.06092718922257464
7	0.10513655213407697
8	0.10597244344963058
9	0.11368121891529172
10	0.13012041478784614
11	0.13606453080956074
12	0.12891301622093537
13	0.12324753063773863
14	0.11228806672270233
15	0.12891301622093537
16	0.11730341461602403
17	0.12343328426341722
18	0.10987326958888077
19	0.09696339260421939
20	0.09148366064670124
21	0.0890688635128797
22	0.10030695786643386
23	0.08061707354450424
24	0.08869735626152253
25	0.0890688635128797
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1076695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.268383604829666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49728500571018	44.954126296446546
2	13.187129111887844	15.1041113725202
3	3.7492798529185265	6.4414559057639265
4	1.4976156520898514	3.4306411062591495
5	0.7851403790850678	2.248186020654253
6	0.4821166138532668	1.6566023510646253
7	0.31102057905920427	1.2468152081391135
8	0.22234071623007723	1.0186474742429312
9	0.17364859434741906	0.8950116882174706
>10	0.9379212600294053	9.887957542495737
>50	0.08428482214919537	3.3867572243249064
>100	0.06684990337746294	7.410021811658732
>500	0.004879553875079208	1.899754569069693
>1k	4.879553875079208E-4	0.4199114291427397
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2025	0.18807554599956347	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1352	0.12556945095872088	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1131	0.10504367532123766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.287681283929061E-5
2	0.0	0.0	0.0	0.0	9.287681283929061E-5
3	0.0	0.0	0.0	0.0	9.287681283929061E-5
4	0.0	0.0	0.0	0.0	9.287681283929061E-5
5	0.0	0.0	0.0	0.0	9.287681283929061E-5
6	0.0	0.0	0.0	0.0	9.287681283929061E-5
7	0.0	0.0	0.0	0.0	9.287681283929061E-5
8	0.0	0.0	0.0	1.8575362567858122E-4	9.287681283929061E-5
9	0.0	0.0	0.0	2.7863043851787184E-4	9.287681283929061E-5
10	0.0	0.0	0.0	2.7863043851787184E-4	9.287681283929061E-5
11	0.0	0.0	0.0	2.7863043851787184E-4	9.287681283929061E-5
12	0.0	0.0	0.0	2.7863043851787184E-4	2.7863043851787184E-4
13	0.0	0.0	0.0	2.7863043851787184E-4	2.7863043851787184E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	565	0.0	13.278537	1
CGCAAGA	210	0.0	13.115067	2
AATCGAA	110	2.73576E-9	12.956638	7
AGACCCG	75	1.4765381E-5	12.667535	5
TAGGACG	75	1.4765381E-5	12.667535	4
CGACCAT	250	0.0	12.5420265	10
GATAATC	70	1.0886539E-4	12.216259	7
GTAAGAC	70	1.0900732E-4	12.214555	3
ATCCCGA	55	0.0030679512	12.090613	16
CATCCCG	55	0.0030679512	12.090613	15
AAGACGG	230	0.0	11.979081	5
CCGTCAA	135	3.7107384E-10	11.961556	18
GCGCAAG	240	0.0	11.870844	1
CGACCCG	105	2.7142414E-7	11.762711	5
GGTCGGC	90	7.4449235E-6	11.612447	11
TTAGGAC	90	7.4526397E-6	11.611366	3
CCCGAAA	90	7.464225E-6	11.609746	18
ATCGAAC	115	7.0476744E-8	11.567086	8
CCGACCA	250	0.0	11.401842	9
TCCGTCA	150	1.7644197E-10	11.398661	17
>>END_MODULE