FastQCFastQC Report
Thu 2 Feb 2017
SRR4062011_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062011_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076695
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10990.10207161731038038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.026.6412451
TAGGACG1301.8189894E-1223.6908554
AACGAAT952.6850103E-823.15755331
ATTTCGT1205.966285E-1022.0027442
AACCGCG2200.020.00249141
GGCGTTA2650.019.9270142
GTCTTAC1352.590241E-919.5598091
CGCGGTC2250.019.55799144
CGGATCG1252.2864697E-819.36061326
GTGTACG808.97241E-519.2541871
GGTATCA7750.019.023491
ACCGTCG2200.018.9988378
CGAAAAC2600.018.61597323
ATCAACG22050.018.2573873
ATACCGT2550.018.1165396
TCAACGC22150.017.9763264
TACCGTC2450.017.9580847
CAACGCA22550.017.6574555
GCGTTAT3000.017.60219243
GCACCGT1002.4839115E-517.598926
Produced by FastQC (version 0.11.5)