##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062011_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1076695
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278481835617328	32.0	32.0	32.0	32.0	32.0
2	31.459991919717282	32.0	32.0	32.0	32.0	32.0
3	31.585090485234907	32.0	32.0	32.0	32.0	32.0
4	31.658413942667142	32.0	32.0	32.0	32.0	32.0
5	31.636523806649052	32.0	32.0	32.0	32.0	32.0
6	35.28742773023001	36.0	36.0	36.0	36.0	36.0
7	35.291685203330566	36.0	36.0	36.0	36.0	36.0
8	35.24142863113509	36.0	36.0	36.0	36.0	36.0
9	35.31467500081267	36.0	36.0	36.0	36.0	36.0
10	35.20501627666145	36.0	36.0	36.0	36.0	36.0
11	35.324493008697914	36.0	36.0	36.0	36.0	36.0
12	35.24476476625228	36.0	36.0	36.0	36.0	36.0
13	35.27898429917479	36.0	36.0	36.0	36.0	36.0
14	35.24610683619781	36.0	36.0	36.0	36.0	36.0
15	35.2270587306526	36.0	36.0	36.0	36.0	36.0
16	35.239198658858825	36.0	36.0	36.0	36.0	36.0
17	35.216862714139104	36.0	36.0	36.0	36.0	36.0
18	35.21426123461147	36.0	36.0	36.0	36.0	36.0
19	35.20373829171678	36.0	36.0	36.0	36.0	36.0
20	35.20226898053767	36.0	36.0	36.0	36.0	36.0
21	35.19334351882381	36.0	36.0	36.0	36.0	36.0
22	35.17642043475636	36.0	36.0	36.0	36.0	36.0
23	35.153278319301194	36.0	36.0	36.0	36.0	36.0
24	35.131698391837986	36.0	36.0	36.0	36.0	36.0
25	35.11363106543636	36.0	36.0	36.0	36.0	36.0
26	35.07829515322352	36.0	36.0	36.0	36.0	36.0
27	35.06517630340997	36.0	36.0	36.0	36.0	36.0
28	35.042259878610004	36.0	36.0	36.0	36.0	36.0
29	35.028309781321546	36.0	36.0	36.0	36.0	36.0
30	35.00517695354766	36.0	36.0	36.0	36.0	36.0
31	35.00083589131555	36.0	36.0	36.0	36.0	36.0
32	34.97922624327224	36.0	36.0	36.0	36.0	36.0
33	34.953340546765794	36.0	36.0	36.0	36.0	36.0
34	34.94833355778563	36.0	36.0	36.0	36.0	36.0
35	34.9394972578121	36.0	36.0	36.0	36.0	36.0
36	34.915550829157745	36.0	36.0	36.0	36.0	36.0
37	34.90613962171274	36.0	36.0	36.0	36.0	36.0
38	34.880906849200564	36.0	36.0	36.0	32.0	36.0
39	34.87014799920126	36.0	36.0	36.0	32.0	36.0
40	34.856309354088204	36.0	36.0	36.0	32.0	36.0
41	34.826742020720815	36.0	36.0	36.0	32.0	36.0
42	34.795315293560385	36.0	36.0	36.0	32.0	36.0
43	34.78412549514951	36.0	36.0	36.0	32.0	36.0
44	34.75248608008768	36.0	36.0	36.0	32.0	36.0
45	34.73845610874017	36.0	36.0	36.0	32.0	36.0
46	34.70582848438973	36.0	36.0	36.0	32.0	36.0
47	34.691846808984906	36.0	36.0	36.0	32.0	36.0
48	34.66690009705627	36.0	36.0	36.0	32.0	36.0
49	34.66282466250888	36.0	36.0	36.0	32.0	36.0
50	34.253461751006554	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	14.0
21	28.0
22	82.0
23	214.0
24	500.0
25	1230.0
26	2515.0
27	4850.0
28	8327.0
29	13020.0
30	19524.0
31	28716.0
32	43290.0
33	73779.0
34	169852.0
35	710750.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.15479564941502	17.764114456023584	11.863398989744148	26.217690904817246
2	16.206323258967547	19.664425166719298	37.31995244552598	26.809299128787174
3	19.070338527323944	22.826841077261577	28.107826464925473	29.994993930489006
4	12.851562282319767	15.764088961209035	34.81973522653605	36.56461352993515
5	14.88750296044841	36.28529899367973	32.97359047826915	15.85360756760271
6	35.35572781124442	34.3382613815996	16.340947381521584	13.965063425634398
7	31.105837772071016	29.749093290114658	19.833007490514955	19.312061447299374
8	28.20213709546343	33.391443259233114	18.728423555417272	19.67799608988618
9	26.938733810410564	14.529091339701585	18.756936736958934	39.77523811292892
10	16.913796386163213	26.579300544722507	30.557493069067842	25.94941000004644
11	37.35477549352416	21.255973140025727	21.658036862807016	19.731214503643095
12	24.690186171571334	23.431055219909076	28.295849799618278	23.582908808901315
13	29.67293430358644	19.163737177195028	25.049990944510746	26.113337574707785
14	23.581608533521564	19.35469190439261	24.891357348181238	32.172342213904585
15	25.30363752037485	27.008391420040027	21.878154909236137	25.809816150348986
16	25.915327610242866	25.680348400794657	23.445816859247454	24.958507129715024
17	24.23156046977092	25.869814571443168	24.967516334709458	24.931108624076455
18	24.980217183744283	25.075509059229567	25.757784027372733	24.18648972965342
19	25.767928707758465	24.89925187727258	25.10237346695211	24.23044594801685
20	25.878569027040182	24.408234837381837	24.853765322366428	24.859430813211553
21	26.512243485852537	24.198403447587292	24.59025072095626	24.699102345603908
22	25.890897607957687	24.240941027867688	25.051848480767536	24.816312883407093
23	24.65612328108079	24.20901388881149	25.39598065931453	25.738882170793186
24	24.807743033239092	24.73520550133558	25.358229526954982	25.09882193847034
25	25.04769228768805	24.302448049306488	25.330688199246953	25.319171463758504
26	24.706622097683347	25.18680057175603	25.59629900351357	24.51027832704705
27	25.239298219543226	24.738411241861634	25.006733600200615	25.015556938394525
28	24.839923096213322	24.612741137024344	25.390509626905178	25.156826139857152
29	24.663342271245032	24.81083174590201	25.392895056780663	25.132930926072294
30	24.69554669708104	25.006687198843856	25.521880143106056	24.77588596096905
31	25.2207235907219	24.718720221122965	24.77946216445649	25.281094023698646
32	25.12875191676659	24.891727648713687	24.70495029641965	25.27457013810007
33	24.787798913523005	24.572788293020004	25.14491158558955	25.494501207867437
34	25.23987892511208	24.703326587343234	25.47643748566206	24.580357001882625
35	25.684644605225277	24.54689836443173	25.36663292126796	24.401824109075037
36	24.54529665428614	25.08862802791144	25.122899699170887	25.24317561863153
37	25.627925077158416	24.780320261990322	25.036477842555037	24.555276818296225
38	24.91522668908094	24.589693460079225	25.34301728901871	25.152062561821133
39	25.426469481902387	24.55091488814319	24.834376500549368	25.188239129405055
40	25.755297461212322	24.667709982864228	25.069959459271196	24.507033096652258
41	24.654794976032782	25.064945076480548	25.524413497430526	24.755846450056147
42	25.608648323314583	25.094274080293655	25.11684413104376	24.180233465348007
43	25.221892391942397	24.321412383620274	25.25300748248267	25.20368774195466
44	24.703316667487112	24.979078681803703	25.139854716422093	25.177749934287093
45	24.985348465048542	25.01311904476636	25.09661654030588	24.90491594987921
46	24.831402546910542	24.844405518089456	25.04400112568579	25.280190809314213
47	25.099007326170625	24.71969595648879	25.42825776826713	24.753038949073456
48	25.661495079189933	25.189754906877987	24.42339771811133	24.72535229582075
49	25.008846845089653	25.30262711825051	24.698719645568914	24.989806391090923
50	25.192420038990193	25.37529686137305	24.633944501667628	24.79833859796913
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	386.0
1	226.5
2	67.0
3	154.5
4	242.0
5	236.5
6	231.0
7	187.5
8	144.0
9	205.0
10	266.0
11	424.0
12	582.0
13	1050.0
14	1518.0
15	1860.5
16	2203.0
17	2148.5
18	2094.0
19	2034.5
20	1975.0
21	2321.5
22	2668.0
23	3214.5
24	3761.0
25	4678.5
26	5596.0
27	7261.0
28	8926.0
29	10552.0
30	12178.0
31	14033.5
32	15889.0
33	18433.5
34	20978.0
35	24208.0
36	27438.0
37	32356.5
38	37275.0
39	40973.5
40	44672.0
41	49088.0
42	53504.0
43	56603.0
44	59702.0
45	65072.0
46	70442.0
47	75022.5
48	79603.0
49	82832.5
50	86062.0
51	84666.5
52	83271.0
53	82474.0
54	81677.0
55	81849.5
56	82022.0
57	79145.0
58	76268.0
59	69960.5
60	63653.0
61	55564.0
62	47475.0
63	40531.5
64	33588.0
65	28065.0
66	22542.0
67	19236.5
68	15931.0
69	14057.5
70	12184.0
71	9587.0
72	6990.0
73	6123.5
74	5257.0
75	3871.5
76	2486.0
77	2207.0
78	1928.0
79	1604.0
80	1280.0
81	992.0
82	704.0
83	630.5
84	557.0
85	418.5
86	280.0
87	190.0
88	100.0
89	65.5
90	31.0
91	25.5
92	20.0
93	18.5
94	17.0
95	16.5
96	16.0
97	12.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031020855488323062
2	0.0032506884493751715
3	1.8575362567858122E-4
4	9.287681283929061E-5
5	0.0
6	9.287681283929061E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.7150725135716244E-4
17	0.0
18	2.786304385178718E-4
19	0.0
20	9.287681283929061E-5
21	0.0
22	0.0
23	9.287681283929061E-5
24	0.0010216449412321965
25	9.287681283929061E-5
26	0.0011145217540714872
27	4.643840641964531E-4
28	0.0023219203209822654
29	7.430145027143249E-4
30	0.0010216449412321965
31	8.358913155536155E-4
32	0.0011145217540714872
33	3.7150725135716244E-4
34	5.572608770357436E-4
35	4.643840641964531E-4
36	3.7150725135716244E-4
37	0.0013002753797500685
38	0.0
39	5.572608770357436E-4
40	0.0
41	0.0014860290054286498
42	0.004365210203446658
43	0.004365210203446658
44	0.0039008261392502056
45	0.0014860290054286498
46	0.001671782631107231
47	6.501376898750342E-4
48	0.003993702952089496
49	0.0037150725135716247
50	0.0013002753797500685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1076695.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.79254431215143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19165677221714	45.97086445621112
2	13.56106966837686	15.945795787964384
3	3.849103847336492	6.7889582551980965
4	1.5002190722300797	3.528067851280867
5	0.7717095584989929	2.268538420708143
6	0.46260269367095874	1.6318553619942273
7	0.2958494884874986	1.2175620913140017
8	0.20815852617361844	0.9790535499211674
9	0.15677024442634896	0.829522938803664
>10	0.8620797396746482	9.270518521323257
>50	0.08041534003853962	3.3065036021305576
>100	0.05607595433616816	6.501981270430095
>500	0.004130239179617353	1.6581366735988308
>1k	1.588553530622059E-4	0.10264121912161289
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1099	0.10207161731038038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7150725135716244E-4
2	0.0	0.0	0.0	0.0	3.7150725135716244E-4
3	0.0	0.0	0.0	0.0	3.7150725135716244E-4
4	0.0	0.0	0.0	0.0	3.7150725135716244E-4
5	0.0	0.0	0.0	0.0	3.7150725135716244E-4
6	0.0	0.0	0.0	0.0	3.7150725135716244E-4
7	0.0	0.0	0.0	9.287681283929061E-5	3.7150725135716244E-4
8	0.0	0.0	0.0	2.7863043851787184E-4	3.7150725135716244E-4
9	0.0	0.0	0.0	3.7150725135716244E-4	3.7150725135716244E-4
10	0.0	0.0	0.0	3.7150725135716244E-4	3.7150725135716244E-4
11	0.0	0.0	0.0	3.7150725135716244E-4	3.7150725135716244E-4
12	0.0	0.0	0.0	3.7150725135716244E-4	5.572608770357437E-4
13	0.0	0.0	0.0	3.7150725135716244E-4	6.501376898750342E-4
14	0.0	0.0	0.0	5.572608770357437E-4	6.501376898750342E-4
15	1.8575362567858122E-4	0.0	0.0	5.572608770357437E-4	6.501376898750342E-4
16	1.8575362567858122E-4	0.0	0.0	5.572608770357437E-4	8.358913155536154E-4
17	2.7863043851787184E-4	0.0	0.0	5.572608770357437E-4	8.358913155536154E-4
18	2.7863043851787184E-4	0.0	0.0	5.572608770357437E-4	9.287681283929061E-4
19	2.7863043851787184E-4	0.0	0.0	5.572608770357437E-4	9.287681283929061E-4
20	2.7863043851787184E-4	0.0	0.0	6.501376898750342E-4	0.0012073985669107778
21	2.7863043851787184E-4	0.0	0.0	8.358913155536154E-4	0.0012073985669107778
22	2.7863043851787184E-4	0.0	0.0	9.287681283929061E-4	0.0013002753797500685
23	2.7863043851787184E-4	0.0	0.0	0.0011145217540714874	0.0013002753797500685
24	2.7863043851787184E-4	0.0	0.0	0.0014860290054286498	0.0013002753797500685
25	2.7863043851787184E-4	0.0	0.0	0.0019504130696251028	0.0013002753797500685
26	2.7863043851787184E-4	0.0	0.0	0.0020432898824643934	0.0013002753797500685
27	2.7863043851787184E-4	0.0	0.0	0.002321920320982265	0.0014860290054286498
28	2.7863043851787184E-4	0.0	0.0	0.0036221957007323336	0.0014860290054286498
29	2.7863043851787184E-4	0.0	0.0	0.005572608770357436	0.0014860290054286498
30	3.7150725135716244E-4	0.0	0.0	0.008916174032571899	0.0014860290054286498
31	3.7150725135716244E-4	0.0	0.0	0.01643919587255444	0.0014860290054286498
32	3.7150725135716244E-4	0.0	0.0	0.02451947858957272	0.0014860290054286498
33	3.7150725135716244E-4	0.0	0.0	0.031949623616715966	0.0014860290054286498
34	3.7150725135716244E-4	0.0	0.0	0.03937976864385922	0.0014860290054286498
35	3.7150725135716244E-4	0.0	0.0	0.04783155861223466	0.0014860290054286498
36	3.7150725135716244E-4	0.0	0.0	0.06074143559689606	0.0014860290054286498
37	3.7150725135716244E-4	0.0	0.0	0.08191734892425431	0.0016717826311072308
38	3.7150725135716244E-4	0.0	0.0	0.1047650448827198	0.0017646594439465215
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1495	0.0	26.641245	1
TAGGACG	130	1.8189894E-12	23.690855	4
AACGAAT	95	2.6850103E-8	23.157553	31
ATTTCGT	120	5.966285E-10	22.00274	42
AACCGCG	220	0.0	20.002491	41
GGCGTTA	265	0.0	19.92701	42
GTCTTAC	135	2.590241E-9	19.559809	1
CGCGGTC	225	0.0	19.557991	44
CGGATCG	125	2.2864697E-8	19.360613	26
GTGTACG	80	8.97241E-5	19.254187	1
GGTATCA	775	0.0	19.02349	1
ACCGTCG	220	0.0	18.998837	8
CGAAAAC	260	0.0	18.615973	23
ATCAACG	2205	0.0	18.257387	3
ATACCGT	255	0.0	18.116539	6
TCAACGC	2215	0.0	17.976326	4
TACCGTC	245	0.0	17.958084	7
CAACGCA	2255	0.0	17.657455	5
GCGTTAT	300	0.0	17.602192	43
GCACCGT	100	2.4839115E-5	17.59892	6
>>END_MODULE