FastQCFastQC Report
Thu 2 Feb 2017
SRR4062010_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062010_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38372
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGGACTGGGAACAAAGGCAGC800.20848535390388823No Hit
GTACTGGCTCTGGTGGGTGTTCTTG690.17981861774210361No Hit
ATATAAAGTCATAATCCCGTTCAAC670.17460648389450642No Hit
AGCCAGTACTGGTCATCAAAGTATC610.1589700823517148No Hit
GCCCCATACCTTCCCGCAGCTGAGG600.1563640154279162No Hit
GCCTAACACAGTCTGGCATTGCGGG550.14333368080892317No Hit
CCCTAGAAGCAGGTAGGGCAGGCGC530.13812154696132595No Hit
GGTCTGCACACAGCACTGAAGGCAG520.13551548003752736No Hit
GCTCTGGGCTGTGCTGAATGGCTGG490.12769727926613156No Hit
GCCCAGAGCTCTTTGAATTCATTGA460.11987907849473575No Hit
GTGTAAATGTATGTGGTAAAAGGCC450.11727301157093714No Hit
GTCCTAGAGAGACTGAGTCTACACA440.11466694464713853No Hit
GTGTAGACTCAGTCTCTCTAGGACT420.10945481079954132No Hit
GTATTGGAATTAGTGAAATTGGAGT420.10945481079954132No Hit
GTGCAGTCCTGGCCCCACCAGGGCT410.10684874387574272No Hit
GTGCCACTCCTATCGCTATCGAAAC410.10684874387574272No Hit
GTACATGGGGAGTCGAGCCCTTGAG410.10684874387574272No Hit
GTCTGCACCATGGCGTATCCCGGGC390.10163661002814553No Hit
CATTTACACCTACTACCCAACTATC390.10163661002814553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCACC250.005927786719.04
GTGTAAA250.005927786719.01
Produced by FastQC (version 0.11.5)