FastQCFastQC Report
Thu 2 Feb 2017
SRR4062010_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062010_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38372
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAAAGTCATAATCCCGTTCAACGGTTGTCAGGGAGTGGCTGTCGGTG750.19545501928489523No Hit
CTCTAGGACTGGGAACAAAGGCAGCTGCCAGGTGCAACAGTTTATGAGGC640.1667882831231106No Hit
GCCTAACACAGTCTGGCATTGCGGGAGGATACAAGCCTTTTAACCTGGAG600.1563640154279162No Hit
CCCTAGAAGCAGGTAGGGCAGGCGCTGAGCATTTGCCACGTGTTGGGGCA600.1563640154279162No Hit
GTACTGGCTCTGGTGGGTGTTCTTGGTTTTAGGCTTTCTCCTGTTTCTCA570.1485458146565204No Hit
GTGTAGACTCAGTCTCTCTAGGACTGGGAACAAAGGCAGCTGCCAGGTGC540.14072761388512456No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA490.12769727926613156No Hit
GCCCAGAGCTCTTTGAATTCATTGAACCCCATCGTGCCGGACATATCTCT450.11727301157093714No Hit
GTCCTAGAGAGACTGAGTCTACACACTGGAATTGAGCTGTGACTTTAAGG440.11466694464713853No Hit
GTCTCAGAGCATGGCAGCTAGTTTTTTGCAATCACAGGAAGCAGGCAACC420.10945481079954132No Hit
GGTCTGCACACAGCACTGAAGGCAGAGAGGAAGCAAGGGCGAGTTCCAGT410.10684874387574272No Hit
GCTCTGGGCTGTGCTGAATGGCTGGAGACAACACTTCATCAGTTTCGATA400.10424267695194411No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT400.10424267695194411No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCTG300.005676808829.33333220
GGGAGGG300.005676808829.3333327
Produced by FastQC (version 0.11.5)