##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062010_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38372
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35082872928177	32.0	32.0	32.0	32.0	32.0
2	31.57362139059731	32.0	32.0	32.0	32.0	32.0
3	31.647685812571666	32.0	32.0	32.0	32.0	32.0
4	31.691545918899198	32.0	32.0	32.0	32.0	32.0
5	31.678906494318774	32.0	32.0	32.0	32.0	32.0
6	35.37629000312728	36.0	36.0	36.0	36.0	36.0
7	35.33172625873033	36.0	36.0	36.0	36.0	36.0
8	35.3116074220786	36.0	36.0	36.0	36.0	36.0
9	35.386662149483996	36.0	36.0	36.0	36.0	36.0
10	35.27996976962368	36.0	36.0	36.0	36.0	36.0
11	35.36711664755551	36.0	36.0	36.0	36.0	36.0
12	35.315803189825914	36.0	36.0	36.0	36.0	36.0
13	35.36099239028458	36.0	36.0	36.0	36.0	36.0
14	35.32138017304285	36.0	36.0	36.0	36.0	36.0
15	35.30459710205358	36.0	36.0	36.0	36.0	36.0
16	35.31478682372563	36.0	36.0	36.0	36.0	36.0
17	35.32982382987595	36.0	36.0	36.0	36.0	36.0
18	35.300062545606174	36.0	36.0	36.0	36.0	36.0
19	35.2772333993537	36.0	36.0	36.0	36.0	36.0
20	35.28067340769311	36.0	36.0	36.0	36.0	36.0
21	35.28614614823309	36.0	36.0	36.0	36.0	36.0
22	35.261466694464715	36.0	36.0	36.0	36.0	36.0
23	35.25737516939435	36.0	36.0	36.0	36.0	36.0
24	35.21903992494527	36.0	36.0	36.0	36.0	36.0
25	35.239080579589285	36.0	36.0	36.0	36.0	36.0
26	35.195272594600226	36.0	36.0	36.0	36.0	36.0
27	35.1767955801105	36.0	36.0	36.0	36.0	36.0
28	35.106718440529555	36.0	36.0	36.0	36.0	36.0
29	35.1211560512874	36.0	36.0	36.0	36.0	36.0
30	35.08498384238507	36.0	36.0	36.0	36.0	36.0
31	35.141587615969975	36.0	36.0	36.0	36.0	36.0
32	35.10254873345148	36.0	36.0	36.0	36.0	36.0
33	35.08313353486918	36.0	36.0	36.0	36.0	36.0
34	35.06562076514125	36.0	36.0	36.0	36.0	36.0
35	35.072370478473886	36.0	36.0	36.0	36.0	36.0
36	35.04917648285208	36.0	36.0	36.0	36.0	36.0
37	35.00946002293339	36.0	36.0	36.0	36.0	36.0
38	35.01667882831231	36.0	36.0	36.0	36.0	36.0
39	35.02783279474617	36.0	36.0	36.0	36.0	36.0
40	34.99040967372042	36.0	36.0	36.0	36.0	36.0
41	34.919602835400816	36.0	36.0	36.0	36.0	36.0
42	34.951631397894296	36.0	36.0	36.0	36.0	36.0
43	34.93735015115188	36.0	36.0	36.0	36.0	36.0
44	34.93555196497446	36.0	36.0	36.0	36.0	36.0
45	34.916006463045974	36.0	36.0	36.0	36.0	36.0
46	34.89799854060252	36.0	36.0	36.0	36.0	36.0
47	34.835765662462215	36.0	36.0	36.0	36.0	36.0
48	34.8465287188575	36.0	36.0	36.0	36.0	36.0
49	34.7957625351819	36.0	36.0	36.0	36.0	36.0
50	34.43375377879704	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	7.0
23	9.0
24	23.0
25	32.0
26	70.0
27	133.0
28	254.0
29	408.0
30	636.0
31	882.0
32	1367.0
33	2315.0
34	5369.0
35	26865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82112040874847	20.231485102056777	12.038268032637314	25.90912645655744
2	15.538585837525085	20.787615001694075	39.154004534910996	24.519794625869842
3	17.742103617220888	25.682789534035233	28.789221307203167	27.785885541540704
4	12.78015219430835	16.96028354008131	36.96445324715938	33.29511101845095
5	13.856457833837174	36.91754404253101	35.645783383717294	13.58021473991452
6	33.201292609194205	37.08693839257792	16.962889607005106	12.748879391222767
7	27.942249556968623	31.00437819243198	22.98551026790368	18.067861982695714
8	24.934848326905033	35.97675388303971	20.379443344105077	18.70895444595017
9	24.726362973001148	16.016887313666214	20.898050661941	38.35869905139164
10	14.911914937975606	28.382674867090586	33.050140727613886	23.65526946731992
11	33.89711247784843	23.553632857291777	24.340665068278952	18.20858959658084
12	22.545084957781718	24.864484519962474	31.28062128635463	21.309809235901177
13	28.364432398623997	20.999687271969144	26.21442718649015	24.42145314291671
14	21.11696028354008	21.0856874804545	27.926613155425834	29.87073908057959
15	24.541332221411448	29.250495152715523	22.52423642239133	23.683936203481707
16	24.31199833211717	28.377462733242993	24.317210465964767	22.993328468675074
17	22.365266340039614	28.29928072552903	26.534973418117374	22.800479516313978
18	22.380902741582403	28.312311060148026	26.863337850516	22.44344834775357
19	23.05326800792244	27.624309392265197	26.720004169707078	22.602418430105285
20	22.545084957781718	28.129886375482123	27.12655060981966	22.1984780569165
21	24.86709058688627	27.830188679245282	25.87042635254873	21.432294381319714
22	23.822057750443033	25.71666840404462	27.752006671531326	22.709267173981026
23	23.196601688731366	27.74418847075993	26.894610653601585	22.164599186907118
24	22.863025122485144	27.4262483060565	26.941519858229963	22.769206713228396
25	23.431758359177504	26.03268093091137	27.523390060201713	23.012170649709415
26	23.39726884186386	27.303763160637967	26.81121651204003	22.487751485458148
27	23.928906494318774	26.634003961221726	27.29333889294277	22.14375065151673
28	23.608360262691548	26.56364015427916	26.787761909725845	23.04023767330345
29	22.281872198478055	27.194308349838426	27.44449077452309	23.07932867716043
30	22.133326383821537	28.726675701032	27.548733451475034	21.59126446367143
31	23.511935786511	28.184613780881897	26.370791201918063	21.932659230689044
32	22.135932450745337	27.40279370374231	27.900552486187845	22.560721359324507
33	23.160116751798185	27.765037006150315	26.915459188991974	22.159387053059522
34	23.056474942013498	26.621667405071538	26.960464934455707	23.361392718459253
35	23.230480558740748	27.87449181694986	26.951944125925152	21.94308349838424
36	23.47284478265402	28.645887626394245	25.956426561034085	21.924841029917648
37	23.296239347423835	26.866644080164708	26.31414349378437	23.52297307862709
38	23.04023767330345	27.57218805378922	27.58782445533201	21.799749817575314
39	22.677994370895444	26.626185760450326	27.87449181694986	22.821328051704366
40	22.753570311685603	26.99885333055353	27.15521734598145	23.092359011779422
41	23.623996664234337	27.116126342124463	26.58709475659335	22.672782237047848
42	21.72326617842529	27.36584221637259	27.21989105788527	23.691000547316847
43	22.696828333897994	26.744155742618126	27.33835448646113	23.220661437022752
44	23.089752944855626	26.743458772021267	27.491399979151467	22.675388303971648
45	22.133903208151988	27.296656328998463	27.778791274660552	22.790649188188997
46	22.894894581845666	26.926585181517293	26.884887024054628	23.293633212582417
47	22.623266965495674	27.647763994579382	26.795580110497237	22.933388929427707
48	21.993171926715487	27.593755700919964	27.72666857783222	22.68640379453233
49	22.673964034401877	26.580661975501695	28.266875162887672	22.478498827208757
50	22.68581257166684	27.684248931512563	26.738246638173667	22.89169185864693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	4.0
4	7.0
5	6.5
6	6.0
7	4.5
8	3.0
9	3.5
10	4.0
11	6.0
12	8.0
13	18.0
14	28.0
15	46.0
16	64.0
17	70.5
18	77.0
19	101.5
20	126.0
21	179.0
22	232.0
23	304.5
24	377.0
25	507.0
26	637.0
27	814.0
28	991.0
29	1053.5
30	1116.0
31	1243.0
32	1370.0
33	1547.0
34	1724.0
35	1939.0
36	2154.0
37	2133.0
38	2112.0
39	2372.5
40	2633.0
41	2585.5
42	2538.0
43	2325.0
44	2112.0
45	2121.0
46	2130.0
47	2274.5
48	2419.0
49	2301.5
50	2184.0
51	2200.0
52	2216.0
53	2154.0
54	2092.0
55	1987.0
56	1882.0
57	1741.0
58	1600.0
59	1527.0
60	1454.0
61	1360.5
62	1267.0
63	1039.0
64	811.0
65	655.5
66	500.0
67	423.5
68	347.0
69	348.0
70	349.0
71	278.5
72	208.0
73	184.5
74	161.0
75	174.5
76	188.0
77	132.5
78	77.0
79	76.5
80	76.0
81	76.0
82	76.0
83	41.5
84	7.0
85	5.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028666736161784633
2	0.00781820077139581
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.002606066923798603
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.002606066923798603
35	0.0
36	0.0
37	0.002606066923798603
38	0.0
39	0.0
40	0.0
41	0.0
42	0.00781820077139581
43	0.002606066923798603
44	0.0
45	0.002606066923798603
46	0.002606066923798603
47	0.0
48	0.002606066923798603
49	0.005212133847597206
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	38372.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3537996455749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.70110272780035	57.04941102887522
2	9.193267556587347	12.384030021890963
3	1.9539562778100212	3.948191389554884
4	0.9286128845037724	2.501824246846659
5	0.5881214935190558	1.9806108620869383
6	0.4875217643644805	1.970186594391744
7	0.3056684078158251	1.4411550088606275
8	0.297929967111627	1.6053372250599396
9	0.2166763397175469	1.313457729594496
>10	1.3039272586573807	14.841551131033045
>50	0.023215322112594312	0.9642447618054831
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATATAAAGTCATAATCCCGTTCAACGGTTGTCAGGGAGTGGCTGTCGGTG	75	0.19545501928489523	No Hit
CTCTAGGACTGGGAACAAAGGCAGCTGCCAGGTGCAACAGTTTATGAGGC	64	0.1667882831231106	No Hit
GCCTAACACAGTCTGGCATTGCGGGAGGATACAAGCCTTTTAACCTGGAG	60	0.1563640154279162	No Hit
CCCTAGAAGCAGGTAGGGCAGGCGCTGAGCATTTGCCACGTGTTGGGGCA	60	0.1563640154279162	No Hit
GTACTGGCTCTGGTGGGTGTTCTTGGTTTTAGGCTTTCTCCTGTTTCTCA	57	0.1485458146565204	No Hit
GTGTAGACTCAGTCTCTCTAGGACTGGGAACAAAGGCAGCTGCCAGGTGC	54	0.14072761388512456	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	49	0.12769727926613156	No Hit
GCCCAGAGCTCTTTGAATTCATTGAACCCCATCGTGCCGGACATATCTCT	45	0.11727301157093714	No Hit
GTCCTAGAGAGACTGAGTCTACACACTGGAATTGAGCTGTGACTTTAAGG	44	0.11466694464713853	No Hit
GTCTCAGAGCATGGCAGCTAGTTTTTTGCAATCACAGGAAGCAGGCAACC	42	0.10945481079954132	No Hit
GGTCTGCACACAGCACTGAAGGCAGAGAGGAAGCAAGGGCGAGTTCCAGT	41	0.10684874387574272	No Hit
GCTCTGGGCTGTGCTGAATGGCTGGAGACAACACTTCATCAGTTTCGATA	40	0.10424267695194411	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	40	0.10424267695194411	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0026060669237986034	0.0
24	0.0	0.0	0.0	0.005212133847597207	0.0
25	0.0	0.0	0.0	0.005212133847597207	0.0
26	0.0	0.0	0.0	0.005212133847597207	0.0
27	0.0	0.0	0.0	0.005212133847597207	0.0
28	0.0	0.0	0.0	0.005212133847597207	0.0
29	0.0	0.0	0.0	0.00781820077139581	0.0
30	0.0	0.0	0.0	0.010424267695194413	0.0
31	0.0	0.0	0.0	0.01563640154279162	0.0
32	0.0	0.0	0.0	0.026060669237986032	0.0
33	0.0	0.0	0.0	0.04430313770457625	0.0
34	0.0	0.0	0.0	0.052121338475972064	0.0
35	0.0	0.0	0.0	0.06515167309496508	0.0
36	0.0	0.0	0.0	0.09121234233295111	0.0
37	0.0	0.0	0.0	0.13030334618993017	0.0
38	0.0	0.0	0.0	0.15897008235171478	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCTG	30	0.0056768088	29.333332	20
GGGAGGG	30	0.0056768088	29.333332	7
>>END_MODULE