FastQCFastQC Report
Thu 2 Feb 2017
SRR4062009_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062009_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15958
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAATCTAGCATGTATGTTGCCT300.18799348289259304No Hit
TTGTTACCCTTGCCAATAACAAAAA260.16292768517358064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG240.15039478631407444No Hit
GTATTACACCGGAGGAGGCCAAGTA240.15039478631407444No Hit
CTTTTGATGTGGTTCATGTGAAAGA220.13786188745456826No Hit
CATCAAAAGAGCCGGGATGTCTCTC210.13159543802481513No Hit
GTGTATGGTGACTGGAGGTGCTAAC210.13159543802481513No Hit
GATTTGGAGACAGGCAAAATAACTG180.11279608973555583No Hit
GCCTAGAAAGGCTTGTACAAACACT180.11279608973555583No Hit
GTTTATGGATGTCATCAGCATTGAC170.10652964030580274No Hit
CCATACACAGGTTCCCAGTGTCAAA170.10652964030580274No Hit
GTTTGTTACCCTTGCCAATAACAAA160.10026319087604962No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers