##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062009_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15958
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.491226970798344	32.0	32.0	32.0	32.0	32.0
2	31.268329364582026	32.0	32.0	32.0	32.0	32.0
3	31.24138363203409	32.0	32.0	32.0	32.0	32.0
4	31.29032460208046	32.0	32.0	32.0	32.0	32.0
5	31.268830680536407	32.0	32.0	32.0	32.0	32.0
6	34.895350294523126	36.0	36.0	36.0	36.0	36.0
7	34.84114550695576	36.0	36.0	36.0	36.0	36.0
8	34.8475999498684	36.0	36.0	36.0	36.0	36.0
9	34.88538663992981	36.0	36.0	36.0	36.0	36.0
10	34.87122446421857	36.0	36.0	36.0	36.0	36.0
11	34.87742824915403	36.0	36.0	36.0	36.0	36.0
12	34.75059531269583	36.0	36.0	36.0	32.0	36.0
13	34.84998120065171	36.0	36.0	36.0	36.0	36.0
14	34.82077954630906	36.0	36.0	36.0	36.0	36.0
15	34.76243890211806	36.0	36.0	36.0	32.0	36.0
16	34.74990600325855	36.0	36.0	36.0	32.0	36.0
17	34.78794335129715	36.0	36.0	36.0	32.0	36.0
18	34.743764882817395	36.0	36.0	36.0	32.0	36.0
19	34.74025567113674	36.0	36.0	36.0	32.0	36.0
20	34.710740694322595	36.0	36.0	36.0	32.0	36.0
21	34.68805614738689	36.0	36.0	36.0	32.0	36.0
22	34.69620253164557	36.0	36.0	36.0	32.0	36.0
23	34.6623637047249	36.0	36.0	36.0	32.0	36.0
24	34.63065547061035	36.0	36.0	36.0	32.0	36.0
25	34.268454693570625	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	7.0
9	1.0
10	1.0
11	0.0
12	0.0
13	0.0
14	2.0
15	3.0
16	1.0
17	6.0
18	5.0
19	10.0
20	19.0
21	20.0
22	24.0
23	42.0
24	47.0
25	77.0
26	113.0
27	118.0
28	222.0
29	256.0
30	349.0
31	464.0
32	762.0
33	1141.0
34	3172.0
35	9095.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64945481889961	19.325730041358565	11.774658478506078	26.250156661235746
2	17.23273593182103	20.760746960772025	36.564732422609346	25.441784684797593
3	20.08397042235869	22.634415340268205	30.060157914525632	27.221456322847477
4	13.81212007269537	16.231121138058533	36.698627561571726	33.258131227674376
5	16.281255875164504	34.43003070752648	33.54640596603372	15.742307451275304
6	32.99899699097292	35.41248746238716	16.856820461384153	14.731695085255767
7	28.691506711830385	29.262325931501692	22.280767783214152	19.76539957345377
8	25.863907180934465	33.044841643148324	21.110065851364062	19.981185324553152
9	25.464124435524337	15.240842950326142	20.45910687405921	38.83592574009032
10	16.544325239977415	26.595143986448335	31.708388230127362	25.152142543446892
11	35.05678609525005	22.614042793499404	22.764635753278533	19.564535357972016
12	22.9089540064002	23.7748635251302	28.957771224195266	24.35841124427433
13	28.987325887815285	20.554649265905383	24.701970134270297	25.756054712009036
14	22.64458662652114	19.834399698908545	25.57395558901016	31.947058085560155
15	25.877110399799157	27.13864306784661	21.822632272641687	25.161614259712543
16	25.175658720200754	27.904642409033876	23.212045169385195	23.707653701380178
17	23.21495796210315	27.073660434182457	26.38348600828209	23.3278955954323
18	24.044915626372248	25.901762750141145	26.654538611128537	23.39878301235807
19	24.592323130958356	25.815353738083292	25.98469643753136	23.607626693426994
20	25.1019000438954	26.061328149495207	24.90123534207061	23.935536464538785
21	24.934152765583846	25.92499686441741	25.548726953467956	23.592123416530793
22	24.23006962303205	25.490811014238226	26.092956156306848	24.18616320642288
23	24.993729621269125	26.185101580135438	24.818159016804614	24.003009781790823
24	23.90418260487866	25.38408478083652	25.816768044146233	24.894964570138583
25	23.98871119473189	25.4499843211038	25.393540294763252	25.167764189401066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	4.0
3	7.0
4	7.0
5	7.0
6	8.5
7	10.0
8	10.0
9	10.0
10	22.0
11	34.0
12	34.0
13	34.0
14	56.5
15	79.0
16	79.0
17	79.0
18	122.5
19	166.0
20	166.0
21	166.0
22	253.0
23	340.0
24	340.0
25	340.0
26	490.5
27	641.0
28	641.0
29	641.0
30	774.5
31	908.0
32	908.0
33	908.0
34	1096.5
35	1285.0
36	1285.0
37	1285.0
38	1383.0
39	1481.0
40	1481.0
41	1481.0
42	1572.0
43	1663.0
44	1663.0
45	1663.0
46	1669.0
47	1675.0
48	1675.0
49	1675.0
50	1736.0
51	1797.0
52	1797.0
53	1797.0
54	1723.0
55	1649.0
56	1649.0
57	1649.0
58	1497.0
59	1345.0
60	1345.0
61	1345.0
62	1174.0
63	1003.0
64	1003.0
65	1003.0
66	901.0
67	799.0
68	799.0
69	799.0
70	665.5
71	532.0
72	532.0
73	532.0
74	412.0
75	292.0
76	292.0
77	292.0
78	213.0
79	134.0
80	134.0
81	134.0
82	101.5
83	69.0
84	69.0
85	69.0
86	41.5
87	14.0
88	14.0
89	14.0
90	9.0
91	4.0
92	4.0
93	4.0
94	2.5
95	1.0
96	1.0
97	1.0
98	15.0
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0062664494297531015
5	0.0062664494297531015
6	0.03759869657851861
7	0.10026319087604962
8	0.08146384258679032
9	0.08773029201654342
10	0.11906253916530894
11	0.13159543802481513
12	0.13159543802481513
13	0.12532898859506206
14	0.10026319087604962
15	0.15666123574382754
16	0.11279608973555583
17	0.12532898859506206
18	0.10652964030580274
19	0.08773029201654342
20	0.06893094372728413
21	0.07519739315703722
22	0.09399674144629652
23	0.06266449429753103
24	0.06893094372728413
25	0.08146384258679032
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	15958.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.00965033212182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.26503420957867	80.27948364456698
2	5.797623334533669	10.088983581902495
3	0.8570399711919338	2.2371224464218575
4	0.29528267915016204	1.0276977064795088
5	0.17284839755131437	0.7519739315703722
6	0.07202016564638099	0.3759869657851861
7	0.12963629816348576	0.789572628148891
8	0.10803024846957147	0.7519739315703722
9	0.050414115952466684	0.3947863140744455
>10	0.25207057976233344	3.3024188494798845
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTAATCTAGCATGTATGTTGCCT	30	0.18799348289259304	No Hit
TTGTTACCCTTGCCAATAACAAAAA	26	0.16292768517358064	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	24	0.15039478631407444	No Hit
GTATTACACCGGAGGAGGCCAAGTA	24	0.15039478631407444	No Hit
CTTTTGATGTGGTTCATGTGAAAGA	22	0.13786188745456826	No Hit
CATCAAAAGAGCCGGGATGTCTCTC	21	0.13159543802481513	No Hit
GTGTATGGTGACTGGAGGTGCTAAC	21	0.13159543802481513	No Hit
GATTTGGAGACAGGCAAAATAACTG	18	0.11279608973555583	No Hit
GCCTAGAAAGGCTTGTACAAACACT	18	0.11279608973555583	No Hit
GTTTATGGATGTCATCAGCATTGAC	17	0.10652964030580274	No Hit
CCATACACAGGTTCCCAGTGTCAAA	17	0.10652964030580274	No Hit
GTTTGTTACCCTTGCCAATAACAAA	16	0.10026319087604962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE