##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062009_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15958
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27823035468104	32.0	32.0	32.0	32.0	32.0
2	31.575260057651334	32.0	32.0	32.0	32.0	32.0
3	31.62482767264068	32.0	32.0	32.0	32.0	32.0
4	31.703972928938462	32.0	32.0	32.0	32.0	32.0
5	31.6624263692192	32.0	32.0	32.0	32.0	32.0
6	35.35687429502444	36.0	36.0	36.0	36.0	36.0
7	35.352926431883695	36.0	36.0	36.0	36.0	36.0
8	35.28788068680286	36.0	36.0	36.0	36.0	36.0
9	35.37291640556461	36.0	36.0	36.0	36.0	36.0
10	35.26469482391277	36.0	36.0	36.0	36.0	36.0
11	35.37128712871287	36.0	36.0	36.0	36.0	36.0
12	35.25498182729665	36.0	36.0	36.0	36.0	36.0
13	35.29057526005765	36.0	36.0	36.0	36.0	36.0
14	35.30548940970046	36.0	36.0	36.0	36.0	36.0
15	35.302168191502695	36.0	36.0	36.0	36.0	36.0
16	35.26883068053641	36.0	36.0	36.0	36.0	36.0
17	35.28361950119063	36.0	36.0	36.0	36.0	36.0
18	35.21857375610979	36.0	36.0	36.0	36.0	36.0
19	35.256987091114176	36.0	36.0	36.0	36.0	36.0
20	35.23461586664995	36.0	36.0	36.0	36.0	36.0
21	35.22985336508334	36.0	36.0	36.0	36.0	36.0
22	35.18811881188119	36.0	36.0	36.0	36.0	36.0
23	35.196891841082845	36.0	36.0	36.0	36.0	36.0
24	35.20585286376739	36.0	36.0	36.0	36.0	36.0
25	35.19858378242888	36.0	36.0	36.0	36.0	36.0
26	35.06980824664745	36.0	36.0	36.0	36.0	36.0
27	35.104649705476874	36.0	36.0	36.0	36.0	36.0
28	35.05965659857125	36.0	36.0	36.0	36.0	36.0
29	35.06579771901241	36.0	36.0	36.0	36.0	36.0
30	35.04900363454067	36.0	36.0	36.0	36.0	36.0
31	35.005389146509586	36.0	36.0	36.0	36.0	36.0
32	35.03327484647199	36.0	36.0	36.0	36.0	36.0
33	35.01403684672265	36.0	36.0	36.0	36.0	36.0
34	34.99348289259306	36.0	36.0	36.0	36.0	36.0
35	34.91765885449304	36.0	36.0	36.0	36.0	36.0
36	34.944855245018175	36.0	36.0	36.0	36.0	36.0
37	34.84640932447675	36.0	36.0	36.0	36.0	36.0
38	34.90506329113924	36.0	36.0	36.0	36.0	36.0
39	34.93363830053892	36.0	36.0	36.0	36.0	36.0
40	34.899862138112546	36.0	36.0	36.0	36.0	36.0
41	34.90036345406693	36.0	36.0	36.0	36.0	36.0
42	34.831871161799725	36.0	36.0	36.0	32.0	36.0
43	34.82591803484146	36.0	36.0	36.0	32.0	36.0
44	34.81169319463592	36.0	36.0	36.0	32.0	36.0
45	34.799097631282116	36.0	36.0	36.0	32.0	36.0
46	34.76576012031583	36.0	36.0	36.0	32.0	36.0
47	34.70134102017797	36.0	36.0	36.0	32.0	36.0
48	34.79577641308435	36.0	36.0	36.0	32.0	36.0
49	34.756673768642685	36.0	36.0	36.0	32.0	36.0
50	34.32817395663617	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	2.0
23	4.0
24	12.0
25	14.0
26	42.0
27	73.0
28	111.0
29	197.0
30	278.0
31	387.0
32	604.0
33	1027.0
34	2328.0
35	10879.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.15471414242728	20.285857572718154	12.456118355065197	26.10330992978937
2	16.20503822534152	20.654217320466223	37.30417345532022	25.836570998872038
3	18.636420604085725	22.947737811755857	29.402180724401557	29.01366085975686
4	13.729790700589046	16.78781802230856	35.95062037849355	33.53177089860885
5	15.446797844341395	35.286376738939715	33.350043865146006	15.916781551572878
6	33.418974808873294	35.562100513848854	17.05100889835819	13.967915778919664
7	28.343150770773278	29.815766386765258	21.75711242010277	20.08397042235869
8	25.892969043739818	33.9516230104023	20.24063165810252	19.91477628775536
9	25.535781426243894	15.31520240631658	19.952374984333876	39.196641183105655
10	16.64995613485399	26.8705351547813	31.658102519112667	24.821406191252038
11	35.41797217696453	22.314826419350794	22.960270710615365	19.306930693069308
12	22.534152149392153	23.768642687053514	29.176588544930443	24.52061661862389
13	29.264318836946984	18.96854242386264	26.024564481764635	25.742574257425744
14	23.01040230605339	19.664118310565236	25.855370347161298	31.47010903622008
15	25.730041358566236	27.8731670635418	21.16806617370598	25.228725404185987
16	24.67101140493796	27.80423611981451	24.113297405689938	23.411455069557586
17	23.781175585913022	26.97079834565735	25.968166436896855	23.279859631532773
18	24.733675899235493	26.44441659355809	25.078330617871913	23.743576889334502
19	23.799974934202282	26.06216317834315	25.748840706855496	24.389021180599073
20	25.053264820152904	25.961899987467103	25.849103897731545	23.13573129464845
21	24.68354430379747	26.50081463842587	24.82767264068179	23.987968417094873
22	24.633412708359444	26.11856122321093	26.174959268078705	23.073066800350922
23	24.457952124326358	26.350419852111795	24.73994234866525	24.451685674896602
24	23.85637297907006	26.1624263692192	25.366587291640556	24.614613360070184
25	24.3451560345908	26.20629151522747	25.097129966161173	24.351422484020553
26	24.27622509086352	25.73630780799599	25.924301290888586	24.06316581025191
27	24.95300162927685	24.93420228098759	25.654843965409196	24.457952124326358
28	24.016044121333668	25.538982201052896	25.839809476059163	24.605164201554274
29	24.056899360822158	25.203659606466978	26.776538413335004	23.96290261937586
30	24.733675899235493	25.128462213309938	26.11856122321093	24.01930066424364
31	23.831307181351047	25.711242010276976	26.030830931194387	24.42661987717759
32	23.731043990474998	25.328988595062036	26.550946233863893	24.389021180599073
33	24.570748214061915	25.42925178593809	25.79270585286377	24.20729414713623
34	24.044366461962653	25.836570998872038	26.149893470359693	23.969169068805616
35	23.768642687053514	25.73630780799599	26.09976187492167	24.395287630028825
36	25.13472866273969	25.579646572252162	25.053264820152904	24.232359944855244
37	24.833939090111542	24.98433387642562	25.836570998872038	24.3451560345908
38	24.313823787442036	25.911768392029078	25.673643313698456	24.10076450683043
39	24.295024439152776	25.303922797343027	25.491916280235614	24.90913648326858
40	24.3451560345908	25.805238751723277	26.19375861636797	23.655846597317957
41	23.89397167564858	26.250156661235746	26.356686301541547	23.49918536157413
42	24.49081907626747	25.844456978128722	25.762988030331517	23.901735915272294
43	24.056899360822158	25.078330617871913	26.11856122321093	24.746208798094997
44	24.16969545055771	25.69870911141747	25.178593808747962	24.95300162927685
45	23.787442035342774	25.69244266198772	26.149893470359693	24.370221832309813
46	24.414086978318082	25.63604461711994	25.79897230229352	24.150896102268455
47	24.232359944855244	26.037097380624136	26.00576513347537	23.724777541045245
48	23.850106529640307	25.67990976312821	26.07469607720266	24.395287630028825
49	24.545682416342903	25.203659606466978	25.460584033086853	24.790073944103273
50	23.46158666499561	25.535781426243894	26.362952750971303	24.639679157789196
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.0
11	3.0
12	3.0
13	7.5
14	12.0
15	16.5
16	21.0
17	21.5
18	22.0
19	29.0
20	36.0
21	59.0
22	82.0
23	98.0
24	114.0
25	142.0
26	170.0
27	197.0
28	224.0
29	286.5
30	349.0
31	391.5
32	434.0
33	509.0
34	584.0
35	649.5
36	715.0
37	762.5
38	810.0
39	855.0
40	900.0
41	890.0
42	880.0
43	926.0
44	972.0
45	1042.5
46	1113.0
47	1039.0
48	965.0
49	942.0
50	919.0
51	886.5
52	854.0
53	852.0
54	850.0
55	788.5
56	727.0
57	719.5
58	712.0
59	694.0
60	676.0
61	636.0
62	596.0
63	564.0
64	532.0
65	495.5
66	459.0
67	403.0
68	347.0
69	307.0
70	267.0
71	240.5
72	214.0
73	188.0
74	162.0
75	129.0
76	96.0
77	78.0
78	60.0
79	51.0
80	42.0
81	30.5
82	19.0
83	13.5
84	8.0
85	6.0
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03759869657851861
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.012532898859506203
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0062664494297531015
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	15958.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.57989723022935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.51574127074986	81.02519112670761
2	5.659702346880366	9.913522997869407
3	0.751287922152261	1.973931570372227
4	0.2933600457927876	1.0276977064795088
5	0.1502575844304522	0.6579771901240757
6	0.12879221522610187	0.6767765384133351
7	0.10732684602175159	0.6579771901240757
8	0.07155123068116771	0.5013159543802482
9	0.10017172295363481	0.789572628148891
>10	0.2218088151116199	2.776037097380624
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTATGGTGACTGGAGGTGCTAACTTGGGAAGAATTGGTGTAATCACCA	33	0.20679283118185238	No Hit
CATCAAAAGAGCCGGGATGTCTCTCTCTGTTGGTGATTACACCAATTCTT	22	0.13786188745456826	No Hit
TTGTTACCCTTGCCAATAACAAAAATGTTGGACAGCCGAGTGGCAAAGCT	22	0.13786188745456826	No Hit
GTATTACACCGGAGGAGGCCAAGTACAAGTTGTGCAAAGTGAGAAAGATC	19	0.11906253916530894	No Hit
CTTCAGCAATGGTGAGGCGGATTCCTTTTCCTCTGGGAAGAGAGATCCAT	19	0.11906253916530894	No Hit
GTCTCCTAGAGACCATTTCACCCACTGCTCTGTTTGGCCGCAAGCCTCTT	17	0.10652964030580274	No Hit
GCCTAGAAAGGCTTGTACAAACACTTTTCCAGCATGTCTCCTAGAGACCA	17	0.10652964030580274	No Hit
GTTTGTTACCCTTGCCAATAACAAAAATGTTGGACAGCCGAGTGGCAAAG	17	0.10652964030580274	No Hit
ATACACAGGTTCCCAGTGTCAAACTTGATGAAGTCAGTTATTTTGCCTGT	16	0.10026319087604962	No Hit
GTTTAATCTAGCATGTATGTTGCCTAGAAAGGCTTGTACAAACACTTTTC	16	0.10026319087604962	No Hit
CTCCTAGAGACCATTTCACCCACTGCTCTGTTTGGCCGCAAGCCTCTTGT	16	0.10026319087604962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0062664494297531015	0.0
24	0.0	0.0	0.0	0.0062664494297531015	0.0
25	0.0	0.0	0.0	0.0062664494297531015	0.0
26	0.0	0.0	0.0	0.0062664494297531015	0.0
27	0.0	0.0	0.0	0.0062664494297531015	0.0
28	0.0	0.0	0.0	0.012532898859506203	0.0
29	0.0	0.0	0.0	0.03133224714876551	0.0
30	0.0	0.0	0.0	0.03133224714876551	0.0
31	0.0	0.0	0.0	0.08773029201654342	0.0
32	0.0	0.0	0.0	0.11279608973555584	0.0
33	0.0	0.0	0.0	0.15666123574382754	0.0
34	0.0	0.0	0.0	0.17546058403308684	0.0
35	0.0	0.0	0.0	0.21305928061160545	0.0
36	0.0	0.0	0.0	0.3195889209174082	0.0
37	0.0	0.0	0.0	0.45745080837197644	0.0
38	0.0	0.0	0.0	0.6454442912645695	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE