FastQCFastQC Report
Thu 2 Feb 2017
SRR4062008_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062008_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596104
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20720.12981610220887863No Hit
GCGCAAGACGGACCAGAGCGAAAGC17580.11014319868880725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA400.005277330514.25075216
ACGAACG400.005277330514.25075215
GGTATCA6450.013.9872811
GTCCTAC3850.013.3199441
GTGTACG500.00150359413.29527951
CCCGTTT655.445139E-513.15578112
TTTGCGG905.397669E-712.66813110
AGAACCG1352.7284841E-1112.6669395
AATCCCG1457.2759576E-1212.446219
CGACCAT2400.012.27225310
GTATCAA16100.012.209951
AACGAAC550.003066569812.09192814
CGCATCG1750.011.94386313
GAACCGC1201.0004442E-811.8756286
ACGGTAT2050.011.5863429
TCCTACA5100.011.5467312
TAGACAG1501.7644197E-1011.4002455
TGAGTCG752.0734638E-411.4002455
CCGACCA2350.011.3201029
AACCGCG1104.962476E-711.2282187
Produced by FastQC (version 0.11.5)