##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062008_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1596104
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.298793812934495	32.0	32.0	32.0	32.0	32.0
2	30.905046914236166	32.0	32.0	32.0	32.0	32.0
3	30.926017978778326	32.0	32.0	32.0	32.0	32.0
4	30.93305887335662	32.0	32.0	32.0	32.0	32.0
5	30.872324735731507	32.0	32.0	32.0	32.0	32.0
6	34.514053595505054	36.0	36.0	36.0	32.0	36.0
7	34.45679855448016	36.0	36.0	36.0	32.0	36.0
8	34.42250567632184	36.0	36.0	36.0	32.0	36.0
9	34.542133845914805	36.0	36.0	36.0	32.0	36.0
10	34.27926688987685	36.0	36.0	36.0	32.0	36.0
11	34.5117204142086	36.0	36.0	36.0	32.0	36.0
12	34.36726303549142	36.0	36.0	36.0	32.0	36.0
13	34.43935858816217	36.0	36.0	36.0	32.0	36.0
14	34.36231285680633	36.0	36.0	36.0	32.0	36.0
15	34.326029506849174	36.0	36.0	36.0	32.0	36.0
16	34.3225905078867	36.0	36.0	36.0	32.0	36.0
17	34.26038403512553	36.0	36.0	36.0	32.0	36.0
18	34.2712887130162	36.0	36.0	36.0	32.0	36.0
19	34.265654368387025	36.0	36.0	36.0	32.0	36.0
20	34.24925631412489	36.0	36.0	36.0	32.0	36.0
21	34.22413201144788	36.0	36.0	36.0	32.0	36.0
22	34.2067960483778	36.0	36.0	36.0	32.0	36.0
23	34.182488735069896	36.0	36.0	36.0	32.0	36.0
24	34.15097637747916	36.0	36.0	36.0	32.0	36.0
25	33.80739851538496	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	20.0
5	116.0
6	317.0
7	80.0
8	274.0
9	232.0
10	111.0
11	27.0
12	74.0
13	67.0
14	188.0
15	275.0
16	474.0
17	670.0
18	899.0
19	1251.0
20	1864.0
21	2602.0
22	3839.0
23	5608.0
24	8099.0
25	11598.0
26	16118.0
27	20973.0
28	28821.0
29	38508.0
30	50803.0
31	70477.0
32	101972.0
33	145897.0
34	331262.0
35	752587.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.780587148444916	17.028081802558383	11.119882562792629	26.071448486204073
2	16.709510601759412	19.48025910608526	37.53222693021691	26.278003361938424
3	19.67859056008571	22.73761900137523	27.98179306369608	29.601997374842977
4	12.69940330173458	15.376117908529658	35.587791339196926	36.33668745053883
5	14.873093647098933	36.36651361944765	33.56722859187784	15.193164141575572
6	35.00129772590775	34.98643970464511	16.560612576782116	13.451649992665027
7	30.711119306379263	29.919669647068268	20.245281030797482	19.123930015754983
8	27.781478303628777	33.77777276011561	19.082671001926784	19.358077934328836
9	26.77731673582296	14.494092703932656	18.474484307323667	40.25410625292072
10	15.92979537243196	26.99494155783456	31.569748219695615	25.505514850037862
11	37.52619374462009	21.20538907278536	21.940256930833456	19.328160251761098
12	24.197378285377308	23.539534314724953	29.138234436118054	23.124852963779684
13	29.415646699333227	19.575468591112028	24.95296680795967	26.055917901595084
14	23.10154987169604	19.713898790724983	25.165264658672577	32.0192866789064
15	24.990417279636638	27.439578673977344	21.986744164891796	25.583259881494214
16	25.513618825926294	26.045098972730983	23.807086443774946	24.634195757567774
17	23.863818138135276	26.16914648903197	25.209063484665332	24.757971888167425
18	24.447775704451725	25.549872202969908	26.06877518699136	23.933576905587003
19	25.39559403071937	25.211154898768566	25.079896448182698	24.313354622329364
20	25.35474913585945	24.9698366034385	24.876461764602595	24.798952496099453
21	26.11809826722988	24.64738830304915	24.61352554004836	24.62098788967261
22	25.593072159569118	24.74038785972227	25.069583203198004	24.59695677751061
23	24.29278631063791	24.823819690685127	25.405329144759737	25.478064853917225
24	24.51426797645886	25.21253813520793	25.415152493799596	24.858041394533615
25	24.690943842517747	24.930864479329575	25.279081754869587	25.099109923283088
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	207.0
1	207.0
2	346.0
3	485.0
4	485.0
5	485.0
6	1067.5
7	1650.0
8	1650.0
9	1650.0
10	2028.5
11	2407.0
12	2407.0
13	2407.0
14	3342.0
15	4277.0
16	4277.0
17	4277.0
18	7108.0
19	9939.0
20	9939.0
21	9939.0
22	15931.5
23	21924.0
24	21924.0
25	21924.0
26	32663.5
27	43403.0
28	43403.0
29	43403.0
30	56248.0
31	69093.0
32	69093.0
33	69093.0
34	84822.0
35	100551.0
36	100551.0
37	100551.0
38	116969.5
39	133388.0
40	133388.0
41	133388.0
42	151654.5
43	169921.0
44	169921.0
45	169921.0
46	187537.5
47	205154.0
48	205154.0
49	205154.0
50	210282.0
51	215410.0
52	215410.0
53	215410.0
54	202587.0
55	189764.0
56	189764.0
57	189764.0
58	174263.0
59	158762.0
60	158762.0
61	158762.0
62	139497.0
63	120232.0
64	120232.0
65	120232.0
66	97666.0
67	75100.0
68	75100.0
69	75100.0
70	56581.5
71	38063.0
72	38063.0
73	38063.0
74	28181.0
75	18299.0
76	18299.0
77	18299.0
78	14447.0
79	10595.0
80	10595.0
81	10595.0
82	7403.5
83	4212.0
84	4212.0
85	4212.0
86	3050.0
87	1888.0
88	1888.0
89	1888.0
90	1244.0
91	600.0
92	600.0
93	600.0
94	374.0
95	148.0
96	148.0
97	148.0
98	390.0
99	632.0
100	632.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010650935026790234
2	6.265255898111902E-5
3	5.638730308300712E-4
4	0.009711146642073449
5	0.032454025552219656
6	0.06302847433500573
7	0.10575751956012891
8	0.10876484239122264
9	0.11835068391533383
10	0.1337632134246891
11	0.13858746046623527
12	0.13213424689118003
13	0.1263702114649171
14	0.11546866620220236
15	0.13088119571155765
16	0.1175988532075604
17	0.1234881937517856
18	0.11145890242741074
19	0.0964849408309233
20	0.0912221258765093
21	0.08971846446096245
22	0.10087061995960162
23	0.08075914852666242
24	0.09078355796364147
25	0.08834010816337783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1596104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.03970656257365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.95534047415114	36.2153975684818
2	16.244262317921155	16.582047640643363
3	5.957821688044229	9.12256412129744
4	2.572762893874039	5.2525225263363495
5	1.3274341228182223	3.3875924054894715
6	0.7693144465790995	2.3559350164647586
7	0.4768554738319799	1.703699441999889
8	0.3171159911786234	1.2948405708845305
9	0.23147520813101324	1.0632984029565822
>10	0.9844627103608055	8.808078073454597
>50	0.08258024811810995	2.9403073698922033
>100	0.07195047991594887	7.466804540711385
>500	0.00640635919905575	2.2752057831714847
>1k	0.002217585876596221	1.5317065382161292
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2072	0.12981610220887863	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1758	0.11014319868880725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.506102359244761E-4
2	0.0	0.0	0.0	0.0	2.506102359244761E-4
3	0.0	0.0	0.0	0.0	2.506102359244761E-4
4	0.0	0.0	0.0	0.0	2.506102359244761E-4
5	6.265255898111902E-5	0.0	0.0	0.0	2.506102359244761E-4
6	6.265255898111902E-5	0.0	0.0	0.0	2.506102359244761E-4
7	6.265255898111902E-5	0.0	0.0	0.0	3.1326279490559515E-4
8	6.265255898111902E-5	0.0	0.0	0.0	3.1326279490559515E-4
9	6.265255898111902E-5	0.0	0.0	0.0	3.1326279490559515E-4
10	6.265255898111902E-5	0.0	0.0	0.0	3.1326279490559515E-4
11	6.265255898111902E-5	0.0	0.0	0.0	3.1326279490559515E-4
12	6.265255898111902E-5	0.0	6.265255898111902E-5	0.0	8.144832667545473E-4
13	6.265255898111902E-5	0.0	6.265255898111902E-5	0.0	0.0010024409436979044
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	40	0.0052773305	14.250752	16
ACGAACG	40	0.0052773305	14.250752	15
GGTATCA	645	0.0	13.987281	1
GTCCTAC	385	0.0	13.319944	1
GTGTACG	50	0.001503594	13.2952795	1
CCCGTTT	65	5.445139E-5	13.155781	12
TTTGCGG	90	5.397669E-7	12.668131	10
AGAACCG	135	2.7284841E-11	12.666939	5
AATCCCG	145	7.2759576E-12	12.4462	19
CGACCAT	240	0.0	12.272253	10
GTATCAA	1610	0.0	12.20995	1
AACGAAC	55	0.0030665698	12.091928	14
CGCATCG	175	0.0	11.943863	13
GAACCGC	120	1.0004442E-8	11.875628	6
ACGGTAT	205	0.0	11.586342	9
TCCTACA	510	0.0	11.546731	2
TAGACAG	150	1.7644197E-10	11.400245	5
TGAGTCG	75	2.0734638E-4	11.400245	5
CCGACCA	235	0.0	11.320102	9
AACCGCG	110	4.962476E-7	11.228218	7
>>END_MODULE