FastQCFastQC Report
Thu 2 Feb 2017
SRR4062008_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062008_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596104
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT18610.1165964122638625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17100.025.9970951
GTTCGCA602.8740687E-421.999179
ACGTAGT550.004485210419.9992456
TGCGCTA808.993478E-519.24927310
GTATTCG708.106423E-418.8635231
TCAACGC24700.018.1693554
AACGCAG24600.018.1537866
GATATAC3700.017.8438721
ATCAACG25450.017.6339133
ATACCGT4650.017.5047156
AATCACG2150.017.39523934
CAACGCA25550.017.392695
TAATACC1903.274181E-1117.3677654
ACCGTCG4700.017.3184978
CGTCGTA4500.017.11046610
GGTATCA9100.016.92881
TATCAAC26950.016.6529542
AATTCGT800.001988812116.49989332
TAGGACG2400.016.4993784
GTAAACG2301.8189894E-1216.26025627
Produced by FastQC (version 0.11.5)