##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062008_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1596104
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.296464390791577	32.0	32.0	32.0	32.0	32.0
2	31.434239874093418	32.0	32.0	32.0	32.0	32.0
3	31.575844681800184	32.0	32.0	32.0	32.0	32.0
4	31.653135384661653	32.0	32.0	32.0	32.0	32.0
5	31.62815330329352	32.0	32.0	32.0	32.0	32.0
6	35.26973179692551	36.0	36.0	36.0	36.0	36.0
7	35.282931438051655	36.0	36.0	36.0	36.0	36.0
8	35.23269598973501	36.0	36.0	36.0	36.0	36.0
9	35.3235622490765	36.0	36.0	36.0	36.0	36.0
10	35.197626219845326	36.0	36.0	36.0	36.0	36.0
11	35.327055129239696	36.0	36.0	36.0	36.0	36.0
12	35.23592635567607	36.0	36.0	36.0	36.0	36.0
13	35.27989592156902	36.0	36.0	36.0	36.0	36.0
14	35.243043686376325	36.0	36.0	36.0	36.0	36.0
15	35.221226812287924	36.0	36.0	36.0	36.0	36.0
16	35.2305808393438	36.0	36.0	36.0	36.0	36.0
17	35.21463075087839	36.0	36.0	36.0	36.0	36.0
18	35.20999571456497	36.0	36.0	36.0	36.0	36.0
19	35.197509059560026	36.0	36.0	36.0	36.0	36.0
20	35.20167106905314	36.0	36.0	36.0	36.0	36.0
21	35.190486334223834	36.0	36.0	36.0	36.0	36.0
22	35.17519033847419	36.0	36.0	36.0	36.0	36.0
23	35.14205214697789	36.0	36.0	36.0	36.0	36.0
24	35.13125147233514	36.0	36.0	36.0	36.0	36.0
25	35.111410659956995	36.0	36.0	36.0	36.0	36.0
26	35.071452110890014	36.0	36.0	36.0	36.0	36.0
27	35.06108436542982	36.0	36.0	36.0	36.0	36.0
28	35.04142399242155	36.0	36.0	36.0	36.0	36.0
29	35.01764546671144	36.0	36.0	36.0	36.0	36.0
30	35.003299910281534	36.0	36.0	36.0	36.0	36.0
31	35.00142785181918	36.0	36.0	36.0	36.0	36.0
32	34.9777953065715	36.0	36.0	36.0	36.0	36.0
33	34.95717259025728	36.0	36.0	36.0	36.0	36.0
34	34.95058780630836	36.0	36.0	36.0	36.0	36.0
35	34.93276628590618	36.0	36.0	36.0	36.0	36.0
36	34.908762837509336	36.0	36.0	36.0	32.0	36.0
37	34.89688892453123	36.0	36.0	36.0	32.0	36.0
38	34.87610957681956	36.0	36.0	36.0	32.0	36.0
39	34.859781693423486	36.0	36.0	36.0	32.0	36.0
40	34.858464110108116	36.0	36.0	36.0	32.0	36.0
41	34.83102980758146	36.0	36.0	36.0	32.0	36.0
42	34.80512360096836	36.0	36.0	36.0	32.0	36.0
43	34.79509480585225	36.0	36.0	36.0	32.0	36.0
44	34.741918446417024	36.0	36.0	36.0	32.0	36.0
45	34.73524093668082	36.0	36.0	36.0	32.0	36.0
46	34.721838927789165	36.0	36.0	36.0	32.0	36.0
47	34.69287339672102	36.0	36.0	36.0	32.0	36.0
48	34.67975583044714	36.0	36.0	36.0	32.0	36.0
49	34.65649481487422	36.0	36.0	36.0	32.0	36.0
50	34.24488692466155	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	9.0
21	33.0
22	81.0
23	249.0
24	660.0
25	1527.0
26	3188.0
27	6346.0
28	11219.0
29	18633.0
30	28600.0
31	43167.0
32	66301.0
33	114768.0
34	264352.0
35	1036969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.33901602275249	17.54380467358483	11.532107550360177	26.585071753302508
2	16.207832274565064	19.882809734360794	37.17438881429441	26.73496917677974
3	19.00124114952625	23.196840300206567	28.293134331724623	29.508784218542562
4	12.40715668099114	15.733445773862965	35.734535929072244	36.12486161607365
5	14.531383919844822	36.77391949396781	33.36975535428769	15.324941231899675
6	35.16483929578347	35.19716809723701	16.33168347847879	13.30630912850072
7	30.62394430438117	30.019472415331332	20.194611378707155	19.161971901580348
8	27.8653521324425	33.750933523128815	19.01987589781117	19.363838446617514
9	26.943858294948196	14.238733816844015	18.374554540305642	40.442853347902144
10	15.87729613940955	27.04693870007136	31.657731361948443	25.418033798570644
11	37.82241006851684	21.088726047926702	21.910790274317964	19.178073609238496
12	24.314079784274707	23.490323938790954	29.06471006901806	23.130886207916276
13	29.557159182609656	19.446602477031572	24.995238405517433	26.00099993484134
14	23.237771473538064	19.474169602983267	25.179374276362942	32.10868464711572
15	25.153185506708837	27.43900146857598	21.87695789246816	25.53085513224702
16	25.68130343118674	26.05603049943769	23.64815377530786	24.61451229406771
17	24.089533012886378	26.1063188864886	25.08345320856285	24.720694892062173
18	24.594042235395982	25.489849646200557	26.016570369562846	23.89953774884061
19	25.515818518091553	25.153624074621707	25.02142717517154	24.3091302321152
20	25.596593703539185	24.834988719399686	24.78311236806146	24.78530520899967
21	26.26902758216257	24.603910522121367	24.518577736789084	24.608484158926988
22	25.670194423421027	24.767872268974955	25.0291960924852	24.532737215118814
23	24.412098976130597	24.715651004760346	25.43032964058688	25.441920378522177
24	24.665949497866976	25.122063842224534	25.36390468964489	24.8480819702636
25	24.944004099996302	24.67184053119512	25.269986385556287	25.114168983252295
26	24.458099977632937	25.684215934244598	25.571127569314395	24.286556518808066
27	25.025765945596106	25.002897689930258	25.14994370649941	24.821392657974222
28	24.753459708988476	25.13677190731338	25.396845286726045	24.712923096972098
29	24.522836914980264	25.06284067414322	25.382056262138963	25.03226614873755
30	24.513811268551606	25.337512718596972	25.608487752555	24.540188260296425
31	25.06528429367657	25.081636693532218	24.85358023577529	24.99949877701592
32	24.84451113498883	25.052518803705293	24.87633881441894	25.22663124688693
33	24.539158524956424	24.83688385829978	25.393991110843793	25.2299665059
34	25.126558803480492	25.05181392597939	25.4242852560247	24.397342014515417
35	25.351325545173292	24.827422877542606	25.609329752934972	24.21192182434913
36	24.376309047724366	25.421855607062955	25.18734693187094	25.014488413341745
37	25.218300597902736	25.147502097327262	25.105524225304638	24.528673079465367
38	24.707788464912063	25.00613995078015	25.31301218466967	24.97305939963812
39	25.334050083547766	24.96220458331689	24.779132544281566	24.924612788853782
40	25.53168561176816	25.073632466075185	25.08741603768679	24.30726588446986
41	24.470439931607824	25.413181846375732	25.685660386106292	24.430717835910155
42	25.450553746907968	25.501931034137076	25.10726575089785	23.94024946805711
43	25.080292775717812	24.773093806272296	25.22101659488457	24.92559682312532
44	24.45811025447437	25.362413731528445	25.15934800928551	25.02012800471168
45	24.720846276551086	25.504331252811586	25.128346179437795	24.64647629119953
46	24.606800073430318	25.140047027983094	25.10740436985573	25.145748528730866
47	24.779805650057327	25.133607753948713	25.508335996090448	24.578250599903516
48	25.428419445365126	25.451852427349923	24.562526902504384	24.557201224780567
49	24.69391527181251	25.628162113223624	24.770040944960826	24.907881670003036
50	24.96845368549922	25.653826345527065	24.64465755175538	24.733062417218335
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	77.0
1	55.0
2	33.0
3	82.0
4	131.0
5	149.5
6	168.0
7	135.5
8	103.0
9	166.5
10	230.0
11	338.0
12	446.0
13	812.0
14	1178.0
15	1663.0
16	2148.0
17	2250.0
18	2352.0
19	2553.5
20	2755.0
21	3412.5
22	4070.0
23	4989.5
24	5909.0
25	7822.0
26	9735.0
27	12714.0
28	15693.0
29	18573.5
30	21454.0
31	24661.5
32	27869.0
33	31996.0
34	36123.0
35	41673.5
36	47224.0
37	54669.5
38	62115.0
39	66970.0
40	71825.0
41	77613.5
42	83402.0
43	86815.0
44	90228.0
45	97012.0
46	103796.0
47	109036.5
48	114277.0
49	119432.0
50	124587.0
51	121497.0
52	118407.0
53	117571.5
54	116736.0
55	116790.5
56	116845.0
57	113488.0
58	110131.0
59	100442.5
60	90754.0
61	79093.0
62	67432.0
63	57625.0
64	47818.0
65	40440.5
66	33063.0
67	28014.5
68	22966.0
69	20093.5
70	17221.0
71	13430.0
72	9639.0
73	8387.0
74	7135.0
75	5228.0
76	3321.0
77	2953.0
78	2585.0
79	2178.0
80	1771.0
81	1368.0
82	965.0
83	850.5
84	736.0
85	571.0
86	406.0
87	271.0
88	136.0
89	90.5
90	45.0
91	33.0
92	21.0
93	18.5
94	16.0
95	12.5
96	9.0
97	9.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031200974372597275
2	0.004197721451734975
3	1.8795767694335707E-4
4	6.265255898111902E-5
5	0.0
6	2.506102359244761E-4
7	0.0
8	0.0
9	0.0
10	6.265255898111902E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.638730308300712E-4
17	0.0
18	1.2530511796223805E-4
19	0.0
20	6.265255898111902E-5
21	0.0
22	0.0
23	1.2530511796223805E-4
24	8.144832667545472E-4
25	3.132627949055951E-4
26	4.385679128678332E-4
27	3.132627949055951E-4
28	0.0010024409436979044
29	2.506102359244761E-4
30	0.0010024409436979044
31	5.012204718489522E-4
32	5.638730308300712E-4
33	1.2530511796223805E-4
34	5.012204718489522E-4
35	3.7591535388671414E-4
36	6.265255898111902E-5
37	0.0015663139745279756
38	0.0
39	6.891781487923092E-4
40	6.265255898111902E-5
41	9.397883847167853E-4
42	0.004260374010716094
43	0.004385679128678332
44	0.004323026569697213
45	0.0018795767694335707
46	0.002067534446376928
47	8.771358257356664E-4
48	0.0039471112158104985
49	0.0036965009798860227
50	0.002380797241282523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1596104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.64275146505355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97113382405942	37.361157590930155
2	16.449743821111763	17.31919551277174
3	5.899121013430741	9.31637884116928
4	2.575771142955779	5.42382720437912
5	1.2962675231977916	3.411954452796094
6	0.7682143517892633	2.426455031587764
7	0.48075515089381615	1.7715791746833238
8	0.30155481685310614	1.2699740221350246
9	0.20955369134267435	0.9928334602743253
>10	0.9057438514103339	8.322420492488272
>50	0.07392611377920018	2.6725119021622787
>100	0.06079511684521736	6.476579096657292
>500	0.0055048514068129955	1.9471403326168937
>1k	0.001914730924108868	1.287992885348531
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1861	0.1165964122638625	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8795767694335707E-4
2	0.0	0.0	0.0	0.0	1.8795767694335707E-4
3	0.0	0.0	0.0	0.0	1.8795767694335707E-4
4	0.0	0.0	0.0	0.0	1.8795767694335707E-4
5	0.0	0.0	0.0	0.0	1.8795767694335707E-4
6	0.0	0.0	0.0	0.0	2.506102359244761E-4
7	0.0	0.0	0.0	0.0	2.506102359244761E-4
8	0.0	0.0	0.0	0.0	2.506102359244761E-4
9	0.0	0.0	0.0	0.0	2.506102359244761E-4
10	0.0	0.0	0.0	0.0	3.1326279490559515E-4
11	0.0	0.0	0.0	0.0	3.7591535388671414E-4
12	0.0	0.0	0.0	0.0	9.397883847167853E-4
13	0.0	0.0	0.0	0.0	0.0011903986206412615
14	0.0	0.0	0.0	0.0	0.0012530511796223806
15	0.0	0.0	0.0	0.0	0.0014410088565657375
16	0.0	0.0	0.0	0.0	0.0014410088565657375
17	0.0	0.0	0.0	0.0	0.0014410088565657375
18	0.0	0.0	0.0	6.265255898111902E-5	0.0014410088565657375
19	0.0	0.0	0.0	6.265255898111902E-5	0.0014410088565657375
20	0.0	0.0	0.0	6.265255898111902E-5	0.0015663139745279756
21	0.0	0.0	0.0	6.265255898111902E-5	0.0017542716514713328
22	0.0	0.0	0.0	6.265255898111902E-5	0.0017542716514713328
23	0.0	0.0	0.0	2.506102359244761E-4	0.0018169242104524516
24	0.0	0.0	0.0	3.1326279490559515E-4	0.0018169242104524516
25	0.0	0.0	0.0	3.1326279490559515E-4	0.0018169242104524516
26	0.0	0.0	0.0	3.7591535388671414E-4	0.0018795767694335707
27	0.0	0.0	0.0	3.7591535388671414E-4	0.0019422293284146897
28	0.0	0.0	0.0	6.265255898111903E-4	0.0019422293284146897
29	0.0	0.0	0.0	9.397883847167853E-4	0.0019422293284146897
30	0.0	0.0	0.0	0.002318144682301404	0.0019422293284146897
31	0.0	0.0	0.0	0.004135068892753856	0.0020048818873958088
32	0.0	0.0	0.0	0.006139950780149664	0.002067534446376928
33	0.0	0.0	0.0	0.008144832667545473	0.002067534446376928
34	0.0	0.0	0.0	0.010087061995960163	0.002318144682301404
35	0.0	0.0	0.0	0.012530511796223805	0.002318144682301404
36	0.0	0.0	0.0	0.01735475883776997	0.002318144682301404
37	0.0	0.0	0.0	0.024685108238560897	0.002318144682301404
38	0.0	0.0	0.0	0.03364442417286092	0.002506102359244761
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1710	0.0	25.997095	1
GTTCGCA	60	2.8740687E-4	21.99917	9
ACGTAGT	55	0.0044852104	19.999245	6
TGCGCTA	80	8.993478E-5	19.249273	10
GTATTCG	70	8.106423E-4	18.863523	1
TCAACGC	2470	0.0	18.169355	4
AACGCAG	2460	0.0	18.153786	6
GATATAC	370	0.0	17.843872	1
ATCAACG	2545	0.0	17.633913	3
ATACCGT	465	0.0	17.504715	6
AATCACG	215	0.0	17.395239	34
CAACGCA	2555	0.0	17.39269	5
TAATACC	190	3.274181E-11	17.367765	4
ACCGTCG	470	0.0	17.318497	8
CGTCGTA	450	0.0	17.110466	10
GGTATCA	910	0.0	16.9288	1
TATCAAC	2695	0.0	16.652954	2
AATTCGT	80	0.0019888121	16.499893	32
TAGGACG	240	0.0	16.499378	4
GTAAACG	230	1.8189894E-12	16.260256	27
>>END_MODULE